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[189280_at]

Description F55A12.4 /REP_DB=WormBase Gene ID /WP=CE26924 /TR=O01758 /GB=AAB54122.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 63      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 35      6grp_total > 63      4grp_total > 02      6grp_highest_sub > 03_04      6grp_cgc > 92      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2049
1 2 3 4 range
A 417.4
(-2.5)
P 		276.4
(-3)
P 		394.1
(-2.6)
P 		1904.8
(3)
P 		1628
B 422.4
(-2.6)
P 		311
(-3)
P 		318.6
(-3)
P 		2133.9
(3)
P 		1823
C 440.5
(-2.7)
P 		334.3
(-3)
P 		402
(-2.8)
P 		2325.6
(3)
P 		1991
avr 426.8
(-2.6) 		307.2
(-3) 		371.6
(-2.8) 		2121.4
(3) 		1814

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2510
1 2 3 4 5 6 range
A 2033.5
(-3)
P 		2168
(-2.7)
P 		4449.6
(3)
P 		4173.7
(2.3)
P 		2442.8
(-2)
P 		2697.4
(-1.4)
P 		2416
B 2154.3
(-3)
P 		2235.4
(-2.8)
P 		4543.7
(3)
P 		3023.5
(-0.8)
P 		3182.9
(-0.4)
P 		2874.4
(-1.2)
P 		2389
C 2150.4
(-3)
P 		2355.8
(-2.3)
P 		4013.4
(3)
P 		3706.7
(2)
P 		2622
(-1.5)
P 		3129.3
(0.2)
P 		1863
avr 2112.7
(-3) 		2253.1
(-2.6) 		4335.6
(3) 		3634.6
(1.1) 		2749.2
(-1.3) 		2900.4
(-0.9) 		2223
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 395
1 2 3 4 range
A 152
(-0.5)
P 		60
(-3)
P 		103
(-1.8)
P 		278.8
(3)
P 		219
B 134.7
(-2)
P 		76.8
(-3)
P 		73.8
(-3)
P 		454.7
(3)
P 		381
C 145.8
(-2)
P 		96.1
(-3)
P 		140.1
(-2.1)
P 		392.1
(3)
P 		296
avr 144.2
(-1.7) 		77.6
(-3) 		105.6
(-2.4) 		375.2
(3) 		298

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 918
1 2 3 4 5 6 range
A 374.9
(-3)
P 		771
(0.5)
P 		969
(2.2)
P 		1058.5
(3)
P 		737.1
(0.2)
P 		656.7
(-0.5)
P 		684
B 749.6
(-2.9)
P 		744.1
(-3)
P 		1103.3
(0.9)
P 		800.8
(-2.4)
P 		1293.2
(3)
P 		1071.9
(0.6)
P 		549
C 797.1
(-1.6)
P 		718.8
(-3)
P 		1021.5
(2.5)
P 		745.4
(-2.5)
P 		848.5
(-0.6)
P 		1047.6
(3)
P 		329
avr 640.5
(-3) 		744.6
(-1.4) 		1031.3
(3) 		868.2
(0.5) 		959.6
(1.9) 		925.4
(1.4) 		391
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189280_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1713
Sequence Type Exemplar sequence
Representative Public ID F55A12.4 NCBI
Target Description F55A12.4 /REP_DB=WormBase Gene ID /WP=CE26924 /TR=O01758 /GB=AAB54122.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059174(11), NM_182015(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059174 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-2) (dhs-2) mRNA, complete cds. 11/11 None
NM_182015 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-2) (dhs-2) mRNA, complete cds. 11/11 None
SNAP00000029042 ENSEMBL cdna:SNAP chromosome:CEL140:I:5354166:5356935:1 11/11 None
GENEFINDER00000029058 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5354371:5356935:1 11/11 None
F55A12.4a ENSEMBL cdna:known chromosome:CEL140:I:5354163:5356950:1 gene:F55A12.4 11/11 None
F55A12.4c ENSEMBL cdna:known chromosome:CEL140:I:5354371:5356935:1 gene:F55A12.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182014 7/11 Cross Hyb Matching Probes None
F55A12.4b 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172183 3 2 NM_059174 189280_at (11)
NM_182015 189280_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5354370-5356935(+) 100.0 100.0
Public Domain and Genome References
Ensembl F55A12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB54122 Dehydrogenases, short chain protein 2, isoform a [Caenorhabditis elegans] ref|NP_491575.1| DeHydrogenases, Short chain family member (dhs-2) [Caenorhabditis elegans] 0.0
blast AAO38639 Dehydrogenases, short chain protein 2, isoform c [Caenorhabditis elegans] ref|NP_871815.1| DeHydrogenases, Short chain family member (dhs-2) [Caenorhabditis elegans] pir||T15192 hypothetical protein F55A12.4 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 2.9E-38
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 2.9E-38
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 7.2E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491575.1 2 31-53,337-359
NP_871815.1 1 7-29
F55A12.4a 2 31-53,337-359
F55A12.4c 1 7-29
GENEFINDER00000029058 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:189280_AT
gtggggaccaatacgtacaacaatggcagttaagaaacttctcaaaaaagcgagaggtcg
tgtggttactgtagccagtatttgtgctcgtgtgggtcttccaggcctcggtccgtattc
ggtgtccaagtacggagtatctgcatattgtgacgtcattcgacaggagcttcgaccatt
tggaatatctgttcatgttttggaaccaggattcttcaatacaccattaattaatagaga
aaagattgatgcagaaattcttgaagcctggaaacatgctccaaatgaagttaagcaaga
atacggggaaaagttcttcaatgatgcacgtgaatccactcatctcttcttgaattccgt
cgcttcttcccaaatatccttggtagttgatgcctacttccacgcgatcacatcaaaaca
tccaagatctcggtaccaaattggatgggatagcattttattattcatcccattctccta
tcttccaaccggactgcaagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGGGACCAATACGTACAACAATG 194 487 492 Antisense
AAGCGAGAGGTCGTGTGGTTACTGT 30 151 539 Antisense
GCCAGTATTTGTGCTCGTGTGGGTC 104 281 565 Antisense
CTCGGTCCGTATTCGGTGTCCAAGT 601 21 598 Antisense
GGTGTCCAAGTACGGAGTATCTGCA 702 499 612 Antisense
GTATCTGCATATTGTGACGTCATTC 69 455 628 Antisense
GTGACGTCATTCGACAGGAGCTTCG 316 481 641 Antisense
GACAGGAGCTTCGACCATTTGGAAT 354 369 653 Antisense
CAGGATTCTTCAATACACCATTAAT 435 207 698 Antisense
TCGCTTCTTCCCAAATATCCTTGGT 184 601 851 Antisense
CTATCTTCCAACCGGACTGCAAGAT 140 225 969 Antisense
Sequence Source Affymetrix Proprietary Database