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[189278_s_at] zinc protease

Description T21D11.1 /REP_DB=WormBase Gene ID /WP=CE00833 /TR=Q22650 /GB=AAA50663.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=zinc protease
Cluster 6grp_total > 82      4grp_total > 41      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 244
1 2 3 4 range
A 17.6
(-3)
A 		179.9
(3)
P 		167.9
(2.6)
P 		113.4
(0.5)
P 		162
B 6.4
(-3)
A 		176.6
(1.8)
P 		219.4
(3)
P 		82.1
(-0.9)
P 		213
C 17.5
(-3)
A 		173.7
(1)
P 		250.6
(3)
P 		73.5
(-1.6)
P 		233
avr 13.8
(-3) 		176.7
(1.9) 		212.6
(3) 		89.7
(-0.7) 		199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 312
1 2 3 4 5 6 range
A 2.7
(-3)
A 		171
(0.2)
P 		198.9
(0.8)
P 		233.7
(1.4)
P 		314.2
(3)
P 		73.1
(-1.6)
P 		312
B 3.7
(-3)
A 		173.8
(1.1)
P 		157.8
(0.7)
P 		197.5
(1.6)
P 		255.6
(3)
P 		114.7
(-0.4)
P 		252
C 23.3
(-3)
A 		213.4
(1.4)
P 		137.3
(-0.4)
P 		281.5
(3)
P 		277.5
(2.9)
P 		142.9
(-0.2)
P 		258
avr 9.9
(-3) 		186.1
(0.9) 		164.7
(0.4) 		237.6
(2) 		282.4
(3) 		110.2
(-0.8) 		273
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 6.4
(-3)
A 		39
(2.2)
P 		43.9
(3)
P 		16.6
(-1.4)
P 		38
B 2
(-3)
A 		43.6
(2.1)
P 		50.8
(3)
P 		17.5
(-1.1)
P 		49
C 5.8
(-3)
A 		49.9
(0.2)
P 		87.3
(3)
P 		12.4
(-2.5)
P 		82
avr 4.7
(-3) 		44.2
(1.2) 		60.7
(3) 		15.5
(-1.8) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 0.5
(-3)
A 		60.8
(0.8)
P 		43.3
(-0.3)
P 		59.3
(0.7)
P 		94.8
(3)
P 		17.8
(-1.9)
P 		94
B 1.3
(-3)
A 		57.9
(0.3)
P 		38.3
(-0.8)
P 		52.3
(-0)
P 		103.9
(3)
P 		42.8
(-0.6)
P 		103
C 8.6
(-3)
A 		65.1
(1.2)
P 		35
(-1)
P 		56.6
(0.5)
P 		89.8
(3)
P 		47.8
(-0.1)
P 		81
avr 3.5
(-3) 		61.3
(0.7) 		38.9
(-0.7) 		56.1
(0.4) 		96.2
(3) 		36.1
(-0.9) 		93
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189278_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7726
Sequence Type Exemplar sequence
Representative Public ID T21D11.1 NCBI
Target Description T21D11.1 /REP_DB=WormBase Gene ID /WP=CE00833 /TR=Q22650 /GB=AAA50663.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=zinc protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066198(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066198 NCBI Caenorhabditis elegans T21D11.1 (T21D11.1) mRNA, complete cds. 11/11 None
SNAP00000016681 ENSEMBL cdna:SNAP chromosome:CEL140:III:7245733:7247322:-1 11/11 None
GENEFINDER00000016682 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7245733:7247819:-1 11/11 None
T21D11.1 ENSEMBL cdna:known chromosome:CEL140:III:7245627:7247708:-1 gene:T21D11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7246114-7247690(-) 100.0 100.0
Public Domain and Genome References
Gene Title zinc protease
Ensembl T21D11.1
Entrez Gene 176025 Entrez gene
SwissProt Q22650 EMBL-EBI
WormBase CE28686 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5578 extracellular matrix (sensu Metazoa) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4222 metalloendopeptidase activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA50663 Hypothetical protein T21D11.1 [Caenorhabditis elegans] ref|NP_498599.1| T21D11.1 [Caenorhabditis elegans] gb|AAF78096.1| MMP-like protein [Caenorhabditis elegans] 1.0E-129
blast AAA50663 Hypothetical protein T21D11.1 [Caenorhabditis elegans] ref|NP_498599.1| T21D11.1 [Caenorhabditis elegans] gb|AAF78096.1| MMP-like protein [Caenorhabditis elegans] 1.0E-111
blast AAA50663 Hypothetical protein T21D11.1 [Caenorhabditis elegans] ref|NP_498599.1| T21D11.1 [Caenorhabditis elegans] gb|AAF78096.1| MMP-like protein [Caenorhabditis elegans] 1.0E-101
blast CAE56445 Hypothetical protein CBG24150 [Caenorhabditis briggsae] 2.0E-74
blast CAE56445 Hypothetical protein CBG24150 [Caenorhabditis briggsae] 3.0E-74
blast CAE56445 Hypothetical protein CBG24150 [Caenorhabditis briggsae] 4.0E-74
blast P91953 50 kDa hatching enzyme precursor (HE) (HEZ) (Envelysin) (Sea-urchin-hatching proteinase) [Contains: 38 kDa hatching enzyme; 32 kDa hatching enzyme non-specific; 15 kDa peptide] dbj|BAA19171.1| hatching enzyme [Hemicentrotus pulcherrimus] 2.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR001818 EMBL-EBI Peptidase M10A and M12B, matrixin and adamalysin 5.5E-19
Pfam IPR001818 EMBL-EBI Peptidase M10A and M12B, matrixin and adamalysin 5.5E-19
Pfam IPR001818 EMBL-EBI Peptidase M10A and M12B, matrixin and adamalysin 5.5E-19
Sequence
Target Sequence 
>C. ELEGANS:189278_S_AT
cgaatggtcaaccgtatcatccgttgattttcgagaaataccacccgatttggtaacaaa
gcaaccacctgatatctacattgcattcgaaaaaggagaacattccgatggattcccatt
tgatgggcaagacggagtagtagctcatgcattctatccacgtgatggacgactacactt
tgacgccgaagaacaatggagccttaattcagttgaaggcgtcaatttattccagacagc
agtacatgaaattggtcatctgttaggtttggagcattcaatggatgttcgagcagcaat
gtttgctgctaaaagaccttatgatcctgcattcactttaggtgatgacgatgtacgagc
cattagatctttattccctataaatgaaacagatgcaaattctggaagcgaagagaattc
agaggatccggtgacaactgttaagcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAATGGTCAACCGTATCATCCGTT 517 253 252 Antisense
GATTTTCGAGAAATACCACCCGATT 600 429 277 Antisense
AAAGCAACCACCTGATATCTACATT 36 123 309 Antisense
GAGAACATTCCGATGGATTCCCATT 246 393 347 Antisense
GTAGTAGCTCATGCATTCTATCCAC 352 455 388 Antisense
TGATGGACGACTACACTTTGACGCC 154 567 414 Antisense
AAGGCGTCAATTTATTCCAGACAGC 498 161 467 Antisense
GGTCATCTGTTAGGTTTGGAGCATT 677 503 505 Antisense
AAGACCTTATGATCCTGCATTCACT 678 153 564 Antisense
ACGAGCCATTAGATCTTTATTCCCT 357 91 606 Antisense
GGATCCGGTGACAACTGTTAAGCCA 414 513 675 Antisense
Sequence Source Affymetrix Proprietary Database