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[189266_at]

Description R53.1 /REP_DB=WormBase Gene ID /WP=CE02303 /TR=Q22017 /GB=CAA91346.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=FAD-1 like protein
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_highest_8_8 > 18      4grp_high_8_8 > 63      6grp_highest_nonorm > 64      6grp_total > 74      4grp_total > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 473
1 2 3 4 range
A 760.4
(1.2)
P 		659.7
(-3)
P 		802.4
(3)
P 		686.9
(-1.9)
P 		143
B 774.5
(-3)
P 		920.6
(-0.6)
P 		1132.3
(3)
P 		792.7
(-2.7)
P 		358
C 985.4
(1.2)
P 		926.1
(0.1)
P 		1087.4
(3)
P 		751.5
(-3)
P 		336
avr 840.1
(-0.8) 		835.5
(-0.9) 		1007.4
(3) 		743.7
(-3) 		264

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 440
1 2 3 4 5 6 range
A 808.4
(-1.3)
P 		752.8
(-3)
P 		848.1
(-0)
P 		946.2
(3)
P 		807
(-1.3)
P 		940.1
(2.8)
P 		193
B 647.6
(-2.2)
P 		603.2
(-3)
P 		749.7
(-0.5)
P 		954.4
(3)
P 		778.4
(-0)
P 		944.1
(2.8)
P 		351
C 600.6
(-3)
P 		600.7
(-3)
P 		941.6
(1.7)
P 		1040.2
(3)
P 		829.2
(0.1)
P 		1037.7
(3)
P 		440
avr 685.5
(-2.4) 		652.2
(-3) 		846.5
(0.6) 		980.3
(3) 		804.9
(-0.2) 		974
(2.9) 		328
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 278
1 2 3 4 range
A 276.9
(3)
P 		143.2
(-1.6)
P 		209.8
(0.7)
P 		100.6
(-3)
P 		176
B 247
(2)
P 		227.4
(0.8)
P 		262.4
(3)
P 		168.9
(-3)
P 		94
C 326.2
(1.7)
P 		266.2
(0.3)
P 		379
(3)
P 		126.7
(-3)
P 		252
avr 283.4
(3) 		212.3
(0.2) 		283.7
(3) 		132.1
(-3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 149
(-3)
P 		267.7
(3)
P 		184.7
(-1.2)
P 		240
(1.6)
P 		243.5
(1.8)
P 		228.9
(1)
P 		119
B 225.3
(-1.5)
P 		200.8
(-2.3)
P 		182
(-3)
P 		252.8
(-0.5)
P 		316.2
(1.7)
P 		352.1
(3)
P 		170
C 222.6
(-1.6)
P 		183.3
(-3)
P 		239.7
(-0.9)
P 		209.2
(-2.1)
P 		268.3
(0.1)
P 		347.4
(3)
P 		164
avr 199
(-3) 		217.3
(-2) 		202.1
(-2.8) 		234
(-1.1) 		276
(1.2) 		309.5
(3) 		111
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189266_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3916
Sequence Type Exemplar sequence
Representative Public ID R53.1 NCBI
Target Description R53.1 /REP_DB=WormBase Gene ID /WP=CE02303 /TR=Q22017 /GB=CAA91346.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=FAD-1 like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027115(11), NM_001027116(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027115 NCBI Caenorhabditis elegans R53.1a (R53.1) mRNA, complete cds. 11/11 None
NM_001027116 NCBI Caenorhabditis elegans R53.1b (R53.1) mRNA, complete cds. 11/11 None
SNAP00000040696 ENSEMBL cdna:SNAP chromosome:CEL140:II:9944048:9946897:1 11/11 None
GENEFINDER00000040707 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9944048:9952517:1 11/11 None
R53.1.2 ENSEMBL cdna:known chromosome:CEL140:II:9943937:9947032:1 gene:R53.1 11/11 None
R53.1.1 ENSEMBL cdna:known chromosome:CEL140:II:9944048:9947032:1 gene:R53.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9944044-9946894(+) 100.0 100.0
Public Domain and Genome References
Ensembl R53.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91346 Hypothetical protein R53.1a [Caenorhabditis elegans] ref|NP_001022286.1| R53.1a [Caenorhabditis elegans] 0.0
blast CAI70410 Hypothetical protein R53.1b [Caenorhabditis elegans] ref|NP_001022287.1| R53.1b [Caenorhabditis elegans] 0.0
blast CAE57921 Hypothetical protein CBG00973 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002500 EMBL-EBI Phosphoadenosine phosphosulfate reductase 1.4E-62
Pfam IPR001453 EMBL-EBI Molybdenum cofactor biosynthesis protein 3.0E-25
Pfam IPR002500 EMBL-EBI Phosphoadenosine phosphosulfate reductase 1.4E-62
Pfam IPR001453 EMBL-EBI Molybdenum cofactor biosynthesis protein 3.0E-25
Sequence
Target Sequence 
>C. ELEGANS:189266_AT
gaaatacggtccgtcaactccgatccaaggattccatatcatggtggaagatcaattccc
agaagccacccaattcatcatagacgcggcgaagttctataacattcaagttctcgaatt
ccctggaccattgaaaaccggacttgcagcactgaaaaagacgcgcccttccatcattcc
agtgctgatgggcagtcgagcaacagatccaaatgggaaatatatgaaaactccagtcga
atggactgattcagattggccgcaagttctccgagtatgcccaatacttaattggacgta
taccgatgtatggcatatgctgagaggtctttgtgtgccgtattgcaagctgtatgacca
gggttacacttcacttggtggtcgggacaacactgtaaaacacccagcccttcgaattgt
gtcatctgatggccgagaacattatcttccagctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATACGGTCCGTCAACTCCGATC 463 363 1068 Antisense
GAAGATCAATTCCCAGAAGCCACCC 637 337 1114 Antisense
GCCACCCAATTCATCATAGACGCGG 413 279 1132 Antisense
TTCTCGAATTCCCTGGACCATTGAA 103 693 1178 Antisense
TGAAAACCGGACTTGCAGCACTGAA 469 573 1199 Antisense
GATTCAGATTGGCCGCAAGTTCTCC 533 431 1315 Antisense
GCAAGTTCTCCGAGTATGCCCAATA 229 319 1329 Antisense
GAGAGGTCTTTGTGTGCCGTATTGC 447 395 1389 Antisense
GGGTTACACTTCACTTGGTGGTCGG 116 495 1428 Antisense
AGCCCTTCGAATTGTGTCATCTGAT 395 83 1473 Antisense
GGCCGAGAACATTATCTTCCAGCTT 353 543 1498 Antisense
Sequence Source Affymetrix Proprietary Database