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[189256_at] cytochrome P450

Description K07C6.3 /REP_DB=WormBase Gene ID /WP=CE17174 /TR=O44651 /GB=AAB94246.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 74      4grp_total > 02      6grp_cgc > 76      6grp_cgc_sub > 03_04      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1657
1 2 3 4 range
A 39.2
(-2.9)
A 		21.5
(-3)
A 		54.7
(-2.9)
A 		1567
(3)
P 		1546
B 55
(-2.9)
A 		62.2
(-2.9)
A 		23.3
(-3)
A 		1671.7
(3)
P 		1648
C 39.4
(-2.9)
A 		68.6
(-2.8)
A 		14.4
(-3)
A 		1470
(3)
P 		1456
avr 44.5
(-2.9) 		50.8
(-2.9) 		30.8
(-3) 		1569.6
(3) 		1539

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 75.5
(-1.6)
A 		46.1
(-3)
A 		150.9
(2.1)
P 		168.5
(3)
P 		128
(1)
P 		161.1
(2.6)
P 		122
B 63.5
(-2.2)
A 		45
(-3)
A 		164.1
(1.9)
P 		190.2
(3)
P 		141.7
(1)
P 		136.6
(0.8)
P 		145
C 45.4
(-3)
A 		68.5
(-2.1)
A 		202.1
(3)
P 		127.5
(0.1)
P 		133.1
(0.4)
P 		138.5
(0.6)
P 		157
avr 61.5
(-2.6) 		53.2
(-3) 		172.4
(3) 		162.1
(2.5) 		134.3
(1.1) 		145.4
(1.6) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 352
1 2 3 4 range
A 14.3
(-2.7)
A 		4.7
(-3)
A 		14.3
(-2.7)
A 		229.4
(3)
P 		225
B 17.5
(-2.8)
A 		15.4
(-2.8)
A 		5.4
(-3)
A 		356.2
(3)
P 		351
C 13
(-2.8)
A 		19.7
(-2.6)
A 		5
(-3)
A 		247.9
(3)
P 		243
avr 14.9
(-2.9) 		13.3
(-2.9) 		8.2
(-3) 		277.8
(3) 		270

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 13.9
(-3)
A 		16.4
(-2.5)
A 		32.9
(1)
P 		42.7
(3)
P 		38.6
(2.1)
P 		39.2
(2.3)
P 		29
B 22.1
(-2)
A 		15
(-3)
A 		39.8
(0.5)
P 		50.4
(2)
P 		57.6
(3)
P 		50.9
(2.1)
P 		43
C 16.8
(-3)
A 		20.9
(-2.3)
A 		51.4
(3)
P 		25.6
(-1.5)
P 		43.1
(1.6)
P 		46.4
(2.1)
P 		35
avr 17.6
(-3) 		17.4
(-3) 		41.4
(2) 		39.6
(1.6) 		46.4
(3) 		45.5
(2.8) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189256_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15154
Sequence Type Exemplar sequence
Representative Public ID K07C6.3 NCBI
Target Description K07C6.3 /REP_DB=WormBase Gene ID /WP=CE17174 /TR=O44651 /GB=AAB94246.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071693(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071693 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-35B2) (cyp-35B2) mRNA, complete cds. 11/11 None
SNAP00000025768 ENSEMBL cdna:SNAP chromosome:CEL140:V:3942028:3944270:1 11/11 None
GENEFINDER00000025780 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3942693:3944270:1 11/11 None
K07C6.3 ENSEMBL cdna:known chromosome:CEL140:V:3942028:3944270:1 gene:K07C6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3942027-3944270(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-35B2
Ensembl K07C6.3
Entrez Gene 187093 Entrez gene
SwissProt O44651 EMBL-EBI
WormBase CE17174 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB94246 Cytochrome p450 family protein 35B2 [Caenorhabditis elegans] ref|NP_504094.1| CYtochrome P450 family member (cyp-35B2) [Caenorhabditis elegans] 0.0
blast CAE58284 Hypothetical protein CBG01391 [Caenorhabditis briggsae] 0.0
blast AAB94245 Cytochrome p450 family protein 35B3 [Caenorhabditis elegans] ref|NP_504095.1| CYtochrome P450 family member (cyp-35B3) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-100
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-140
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.4E-91
Pfam IPR001128 EMBL-EBI Cytochrome P450 6.2E-63
Sequence
Target Sequence 
>C. ELEGANS:189256_AT
atcggagatctgtggctagctggacaagatactacaacaacgacattggtttcaggattc
aatcaacttgttaaccatccagaagttatcagaaaatgcagagaagaaattctgagaatc
acggaaaatggctcacgtccacttcagctaaaagacagagctgaatctcattacctcaac
gctacaattactgaaattcaacgccacgcttcaattttaaacgtcaacttttggagactg
ataaatgagcccacaaatgtaaaaggatacgagattgattcgggaacagtcatgacagcc
caacttggagccctccacgtaaacaacgatattttcaaaaacgccgacaagttctatccg
gagagattcattgaaaatggaaaacttttgaatcaagttatcccgtttggaatcggaaaa
cgatcttgcgttggggagaatctcgcaagatcagaattgtatttactgactggaaaccta
ttgctccggtataatatcagaccacatggatcgttaccttcaacagctgaccagttgcca
tatagtgccggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGGAGATCTGTGGCTAGCTGGAC 110 35 916 Antisense
ACGGAAAATGGCTCACGTCCACTTC 582 93 1036 Antisense
TCATGACAGCCCAACTTGGAGCCCT 541 621 1205 Antisense
GAGCCCTCCACGTAAACAACGATAT 16 387 1223 Antisense
CAAAAACGCCGACAAGTTCTATCCG 154 189 1251 Antisense
GAATCAAGTTATCCCGTTTGGAATC 407 327 1305 Antisense
ACGATCTTGCGTTGGGGAGAATCTC 568 89 1335 Antisense
GACTGGAAACCTATTGCTCCGGTAT 464 375 1383 Antisense
GACCACATGGATCGTTACCTTCAAC 349 383 1415 Antisense
TACCTTCAACAGCTGACCAGTTGCC 203 649 1430 Antisense
GACCAGTTGCCATATAGTGCCGGAA 489 383 1444 Antisense
Sequence Source Affymetrix Proprietary Database