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[189255_at] Cytochrome P450

Description Y17D7A.4 /REP_DB=WormBase Gene ID /WP=CE20198 /TR=O45317 /GB=CAA16294.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Cytochrome P450
Cluster 6grp_total > 34      4grp_total > 32      6grp_cgc > 21      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 30.2
(-3)
A 		61.3
(2.1)
A 		66.6
(3)
A 		55.6
(1.2)
A 		36
B 41.6
(-2.3)
A 		39.7
(-3)
P 		56.7
(3)
A 		45.1
(-1.1)
A 		17
C 59.5
(-0.4)
A 		49.3
(-3)
A 		56.7
(-1.1)
A 		73
(3)
P 		24
avr 43.8
(-3) 		50.1
(-0.7) 		60
(3) 		57.9
(2.2) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 85.5
(3)
A 		24.5
(-3)
A 		36.1
(-1.9)
A 		52.2
(-0.3)
A 		45.6
(-0.9)
A 		39
(-1.6)
A 		61
B 51.6
(1.7)
A 		56.2
(2.3)
A 		62.1
(3)
A 		13.8
(-3)
A 		48.7
(1.3)
A 		34.9
(-0.4)
P 		48
C 36.5
(-1.9)
A 		75.3
(3)
A 		54.9
(0.4)
A 		28.8
(-2.8)
A 		27.4
(-3)
A 		35.8
(-1.9)
A 		48
avr 57.9
(3) 		52
(1.7) 		51
(1.4) 		31.6
(-3) 		40.6
(-0.9) 		36.6
(-1.9) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 11
(-1.1)
A 		13.3
(0.4)
A 		17.4
(3)
A 		8.1
(-3)
A 		9
B 13.3
(3)
A 		9.8
(-2.7)
P 		13.1
(2.7)
A 		9.6
(-3)
A 		4
C 19.7
(2.9)
A 		14.2
(-1.5)
A 		19.8
(3)
A 		12.3
(-3)
P 		8
avr 14.7
(1.1) 		12.4
(-0.9) 		16.8
(3) 		10
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 15.8
(3)
A 		8.7
(-2.4)
A 		7.9
(-3)
A 		13.2
(1)
A 		13.8
(1.5)
A 		9.5
(-1.8)
A 		8
B 18
(2.3)
A 		18.7
(2.6)
A 		15.1
(1.2)
A 		3.7
(-3)
A 		19.8
(3)
A 		13
(0.5)
P 		16
C 13.5
(-0.3)
A 		23
(3)
A 		14
(-0.1)
A 		5.8
(-3)
A 		8.9
(-1.9)
A 		12
(-0.8)
A 		17
avr 15.8
(2.3) 		16.8
(3) 		12.3
(0.1) 		7.6
(-3) 		14.2
(1.3) 		11.5
(-0.5) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189255_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13067
Sequence Type Exemplar sequence
Representative Public ID Y17D7A.4 NCBI
Target Description Y17D7A.4 /REP_DB=WormBase Gene ID /WP=CE20198 /TR=O45317 /GB=CAA16294.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075278(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075278 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33D3) (cyp-33D3) mRNA, complete cds. 11/11 None
SNAP00000040259 ENSEMBL cdna:SNAP chromosome:CEL140:V:18834900:18836258:-1 9/11 None
GENEFINDER00000040260 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18834900:18836307:-1 11/11 None
Y17D7A.4 ENSEMBL cdna:known chromosome:CEL140:V:18834900:18839770:-1 gene:Y17D7A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18834899-18839770(-) 100.0 100.0
Public Domain and Genome References
Gene Title Cytochrome P450
Gene Symbol cyp-33D3
Ensembl Y17D7A.4
Entrez Gene 189444 Entrez gene
SwissProt O45317 EMBL-EBI
WormBase CE16589 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:267502_AT cytochrome P450 family protein at
ATGENOME1:17403_AT cytochrome P450 family protein at
CHICKEN:GGAAFFX.5144.1.S1_S_AT similar to Cytochrome P450 2J6 (CYPIIJ6) (Arachidonic acid epoxygenase) gga
CHICKEN:GGAAFFX.5144.2.S1_AT similar to Cytochrome P450 2J6 (CYPIIJ6) (Arachidonic acid epoxygenase) gga
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04001 Hypothetical protein Y17D7A.4 [Caenorhabditis elegans] emb|CAA16294.2| Hypothetical protein Y17D7A.4 [Caenorhabditis elegans] ref|NP_507679.2| CYtochrome P450 family member (cyp-33D3) [Caenorhabditis elegans] 0.0
blast CAE63115 Hypothetical protein CBG07412 [Caenorhabditis briggsae] 0.0
blast CAB07250 Hypothetical protein K05D4.4 [Caenorhabditis elegans] ref|NP_507076.1| CYtochrome P450 family member (cyp-33D1) [Caenorhabditis elegans] 0.0
blast CAB04001 Hypothetical protein Y17D7A.4 [Caenorhabditis elegans] emb|CAA16294.2| Hypothetical protein Y17D7A.4 [Caenorhabditis elegans] ref|NP_507679.2| CYtochrome P450 family member (cyp-33D3) [Caenorhabditis elegans] 1.0E-112
blast CAB04001 Hypothetical protein Y17D7A.4 [Caenorhabditis elegans] emb|CAA16294.2| Hypothetical protein Y17D7A.4 [Caenorhabditis elegans] ref|NP_507679.2| CYtochrome P450 family member (cyp-33D3) [Caenorhabditis elegans] 1.0E-107
blast CAB07250 Hypothetical protein K05D4.4 [Caenorhabditis elegans] ref|NP_507076.1| CYtochrome P450 family member (cyp-33D1) [Caenorhabditis elegans] 4.0E-87
blast CAE63115 Hypothetical protein CBG07412 [Caenorhabditis briggsae] 1.0E-84
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-148
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 8.3E-106
Pfam IPR001128 EMBL-EBI Cytochrome P450 8.8E-62
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.2E-62
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507679.2 2 2-20,296-318
Y17D7A.4 2 2-20,296-318
GENEFINDER00000040260 1 10-32
Sequence
Target Sequence 
>C. ELEGANS:189255_AT
tatgcgccatgctatttgatctctggattgcgggactaatgacaaccacaatgactatga
catggggtctgtcttattacctctataacccggaagttcaacgaaaaatccgtgaagaat
tggacaaagttatcggaaatgaccgactgattagcacggctgacaagaatgatctaccgt
atctacaggcttttgtaactgaaactcagcgaactgcaaacattattccgttgaacttaa
tccacatgacaactagggatactgtaattgatggattcccaattcaaaagggaaccggcg
tgattgctcagattagtacggttatgtacgatgaaaaggtttttccggagccatacaaat
tcaagcctgaaagatttattgaaaatgggaaattcaaaaaagtcgatgaagtaattccat
tttccatcggaaaacgccagtgtttgggagaagggctcgccagaatagagctttttttat
ttttcgcgaatattttcaatcgatatgacgtcatgccagacttttctggctcgctgccag
atttggacaagtcaaaggacaattttgtgatcccgcgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGCGCCATGCTATTTGATCTCTG 278 657 959 Antisense
TGATCTCTGGATTGCGGGACTAATG 346 563 975 Antisense
ATGGGGTCTGTCTTATTACCTCTAT 370 51 1020 Antisense
GACCGACTGATTAGCACGGCTGACA 391 381 1099 Antisense
GATCTACCGTATCTACAGGCTTTTG 428 419 1129 Antisense
TTCCGTTGAACTTAATCCACATGAC 557 697 1184 Antisense
GAACCGGCGTGATTGCTCAGATTAG 9 347 1250 Antisense
GTTTTTCCGGAGCCATACAAATTCA 66 449 1297 Antisense
TATGACGTCATGCCAGACTTTTCTG 579 655 1462 Antisense
GGCTCGCTGCCAGATTTGGACAAGT 424 539 1486 Antisense
AGGACAATTTTGTGATCCCGCGCAA 162 55 1514 Antisense
Sequence Source Affymetrix Proprietary Database