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[189253_s_at] cytochrome P450 /// cytochrome P450

Description F42A6.4 /REP_DB=WormBase Gene ID /WP=CE17056 /TR=O44485 /GB=AAB92049.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 41      4grp_highest_8_8 > 45      4grp_high_8_8 > 09      6grp_highest_nonorm > 71      6grp_total > 30      4grp_total > 23      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 148
1 2 3 4 range
A 322.2
(3)
P 		228.2
(-1.1)
P 		185.4
(-3)
P 		282.7
(1.3)
P 		137
B 289.3
(3)
P 		186.3
(-3)
P 		259.4
(1.3)
P 		287.2
(2.9)
P 		103
C 318.4
(2.2)
P 		241.8
(-1.7)
P 		215.2
(-3)
P 		333.5
(3)
P 		118
avr 310
(3) 		218.8
(-3) 		220
(-2.9) 		301.1
(2.4) 		91

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 359
1 2 3 4 5 6 range
A 201.6
(-1.7)
P 		299.5
(-0.1)
P 		484.3
(3)
P 		333.5
(0.5)
P 		204.7
(-1.7)
P 		125
(-3)
P 		359
B 307.2
(0.9)
P 		358.7
(2.5)
P 		373.8
(3)
P 		285.1
(0.2)
P 		266
(-0.4)
P 		180.9
(-3)
P 		193
C 272.1
(-2)
P 		395.8
(3)
P 		395
(3)
P 		359.7
(1.5)
P 		248.2
(-3)
P 		250.5
(-2.9)
P 		148
avr 260.3
(-1.1) 		351.3
(1.3) 		417.7
(3) 		326.1
(0.6) 		239.6
(-1.6) 		185.5
(-3) 		232
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 117.3
(3)
P 		49.5
(-2.4)
P 		48.5
(-2.4)
P 		41.4
(-3)
P 		76
B 92.3
(3)
P 		46
(-3)
P 		60.1
(-1.2)
P 		61.2
(-1)
P 		46
C 105.4
(3)
P 		69.5
(-1.4)
P 		75
(-0.7)
P 		56.2
(-3)
P 		49
avr 105
(3) 		55
(-2.8) 		61.2
(-2) 		52.9
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 37.2
(-2.5)
P 		106.5
(3)
P 		105.5
(2.9)
P 		84.6
(1.3)
P 		61.8
(-0.5)
P 		30.4
(-3)
P 		76
B 106.9
(1.6)
P 		119.4
(3)
P 		90.8
(-0.3)
P 		75.5
(-2.1)
P 		108.1
(1.7)
P 		67.4
(-3)
P 		52
C 100.8
(0.5)
P 		120.8
(3)
P 		100.5
(0.5)
P 		72.3
(-3)
P 		80.3
(-2)
P 		83.8
(-1.6)
P 		49
avr 81.6
(-0.7) 		115.6
(3) 		98.9
(1.2) 		77.5
(-1.1) 		83.4
(-0.5) 		60.5
(-3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189253_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10575
Sequence Type Exemplar sequence
Representative Public ID F42A6.4 NCBI
Target Description F42A6.4 /REP_DB=WormBase Gene ID /WP=CE17056 /TR=O44485 /GB=AAB92049.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065378(11), NM_067921(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065378 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-25A4) (cyp-25A4) mRNA, complete cds. 11/11 None
NM_067921 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-25A5) (cyp-25A5) mRNA, complete cds. 11/11 None
SNAP00000028396 ENSEMBL cdna:SNAP chromosome:CEL140:III:3849884:3852048:-1 11/11 None
GENEFINDER00000028413 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3849884:3852048:-1 11/11 None
SNAP00000037842 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3327477:3328888:-1 11/11 None
GENEFINDER00000037856 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3327477:3336850:-1 11/11 None
C36A4.6 ENSEMBL cdna:known chromosome:CEL140:III:3849884:3852048:-1 gene:C36A4.6 11/11 None
F42A6.4 ENSEMBL cdna:known chromosome:CEL140:IV:3327477:3329641:-1 gene:F42A6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3849883-3852048(-) 98.61 98.61
chrIV:3327482-3329647(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
cytochrome P450
Gene Symbol cyp-25A4
cyp-25A5
Ensembl C36A4.6
F42A6.4
Entrez Gene 183248 Entrez gene
185648 Entrez gene
SwissProt Q27479 EMBL-EBI
O44485 EMBL-EBI
WormBase CE03074 Wormbase
CE17056 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1628052_AT dm
DROSGENOME1:152313_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91272 Hypothetical protein C36A4.6 [Caenorhabditis elegans] ref|NP_497779.1| CYtochrome P450 family member (cyp-25A4) [Caenorhabditis elegans] 0.0
blast AAB92049 Cytochrome p450 family protein 25A5 [Caenorhabditis elegans] ref|NP_500322.1| CYtochrome P450 family member (cyp-25A5) [Caenorhabditis elegans] 0.0
blast CAA91267 Hypothetical protein C36A4.2 [Caenorhabditis elegans] ref|NP_497776.2| CYtochrome P450 family member (cyp-25A2) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 CP14_CHICK Animalia; CYP1A4; chi; CP14_CHICK; Gallus gallus; P79760 1.0E-112
p450 CP12_RABIT Animalia; CYP1A2; rab; CP12_RABIT; Orytolagus cuniculus; P00187 1.0E-105
p450 CP12_RAT Animalia; CYP1A2; rat; CP12_RAT; Rattus sp.; P04799 8.0E-88
p450 CP12_RAT Animalia; CYP1A2; rat; CP12_RAT; Rattus sp.; P04799 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.1E-73
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.0E-20
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.3E-62
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.7E-62
Pfam IPR001357 EMBL-EBI BRCT 4.2E-5
Pfam IPR008493 EMBL-EBI Protein of unknown function DUF775 3.7E-26
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.2E-79
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497779.1 1 3-22
NP_500322.1 1 3-22
C36A4.6 1 3-22
F42A6.4 1 3-22
SNAP00000028396 1 3-22
GENEFINDER00000028413 1 3-22
Sequence
Target Sequence 
>C. ELEGANS:189253_S_AT
ttgaaaactgtttcgcatttttgattgctggatatgaaacaacaagtactgcaatgatgt
tttctgcatacctattggcagaatatccaatagttcaacagaagttgtatgaagaaatca
agaaaactaaggaaagtgcaggattaaattatgattccatacataatatgaaatattcgg
attgtgtttataaagaatctttgaggttttatccaccaactacacacttcaccaatcgag
tctgtctgaacgatatgacaatacaaggccagatttaccccaaagattcaaccctcaaag
tccaaccgcacaccatccaccgtaaccctgcaaactgggaatcgccggatgaattccaac
cggaaagatttgagaattgggaagaaaagagtagctcattgaagtggattccatttggtg
ttggaccaagatattgtgttggaatgagatttgctgaaatggaatttaaaaccacaattg
ccaagttactggatacttttgagctgagcttggtgccaggagaccctccaatgattccag
agac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAAAACTGTTTCGCATTTTTGAT 294 701 842 Antisense
TGATGTTTTCTGCATACCTATTGGC 110 567 896 Antisense
AGGTTTTATCCACCAACTACACACT 135 53 1045 Antisense
ACTACACACTTCACCAATCGAGTCT 621 99 1060 Antisense
TCACCAATCGAGTCTGTCTGAACGA 705 623 1070 Antisense
TCCACCGTAACCCTGCAAACTGGGA 600 587 1157 Antisense
AACCCTGCAAACTGGGAATCGCCGG 336 145 1165 Antisense
GAATCGCCGGATGAATTCCAACCGG 550 251 1180 Antisense
ACTGGATACTTTTGAGCTGAGCTTG 528 97 1329 Antisense
TGAGCTGAGCTTGGTGCCAGGAGAC 282 569 1341 Antisense
GAGACCCTCCAATGATTCCAGAGAC 639 393 1361 Antisense
Sequence Source Affymetrix Proprietary Database