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[189249_at] protein kinase

Description T11F8.4 /REP_DB=WormBase Gene ID /WP=CE17237 /TR=O44192 /GB=AAB88557.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 58      4grp_total > 10      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 9.4
(-3)
A 		48.8
(1.8)
A 		17.1
(-2.1)
A 		59
(3)
A 		50
B 41.7
(-0.9)
A 		12.7
(-2.9)
A 		11.2
(-3)
A 		97.7
(3)
A 		87
C 13.2
(-2.8)
A 		11.1
(-3)
A 		32.9
(-0.5)
A 		62.9
(3)
A 		52
avr 21.4
(-2.9) 		24.2
(-2.6) 		20.4
(-3) 		73.2
(3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 31.6
(-1.3)
A 		11.3
(-3)
A 		10.9
(-3)
A 		38.3
(-0.7)
A 		34.4
(-1.1)
A 		83.8
(3)
A 		73
B 77
(2.4)
A 		80.8
(3)
A 		78.9
(2.7)
A 		46.5
(-2.4)
A 		46.1
(-2.5)
A 		43
(-3)
A 		38
C 48.8
(-1.7)
A 		55.7
(-0.2)
A 		42.5
(-3)
A 		68.3
(2.4)
A 		56.7
(-0)
A 		71.1
(3)
A 		29
avr 52.5
(-0.7) 		49.3
(-1.6) 		44.1
(-3) 		51
(-1.1) 		45.7
(-2.6) 		66
(3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 3.4
(-3)
A 		10.6
(3)
A 		4.5
(-2.1)
A 		8.6
(1.3)
A 		7
B 13.3
(0.5)
A 		3.1
(-2.8)
A 		2.6
(-3)
A 		20.8
(3)
A 		18
C 4.4
(-2.1)
A 		3.2
(-3)
A 		11.5
(3)
A 		10.6
(2.3)
A 		8
avr 7
(-1.9) 		5.6
(-3) 		6.2
(-2.5) 		13.3
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 5.8
(-1.9)
A 		4
(-2.5)
A 		2.4
(-3)
A 		9.7
(-0.6)
A 		10.4
(-0.3)
A 		20.4
(3)
A 		18
B 26.8
(3)
A 		26.9
(3)
A 		19.2
(-0.2)
A 		12.3
(-3)
A 		18.7
(-0.4)
A 		16
(-1.5)
A 		15
C 18.1
(0.4)
A 		17
(-0.1)
A 		10.8
(-3)
A 		13.7
(-1.7)
A 		18.4
(0.5)
A 		23.8
(3)
A 		13
avr 16.9
(0.9) 		16
(0.4) 		10.8
(-3) 		11.9
(-2.3) 		15.8
(0.2) 		20.1
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189249_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10712
Sequence Type Exemplar sequence
Representative Public ID T11F8.4 NCBI
Target Description T11F8.4 /REP_DB=WormBase Gene ID /WP=CE17237 /TR=O44192 /GB=AAB88557.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068415(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068415 NCBI Caenorhabditis elegans T11F8.4 (T11F8.4) mRNA, complete cds. 11/11 None
SNAP00000002004 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5474165:5476502:-1 11/11 None
GENEFINDER00000002012 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5474165:5476502:-1 11/11 None
T11F8.4 ENSEMBL cdna:known chromosome:CEL140:IV:5474149:5476555:-1 gene:T11F8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5474170-5476508(-) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl T11F8.4
Entrez Gene 188418 Entrez gene
SwissProt O44192 EMBL-EBI
WormBase CE35006 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4289 subtilase activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_500816 T11F8.4 [Caenorhabditis elegans] gb|AAB88557.3| Hypothetical protein T11F8.4 [Caenorhabditis elegans] 0.0
blast CAE72513 Hypothetical protein CBG19692 [Caenorhabditis briggsae] 1.0E-162
blast CAE68924 Hypothetical protein CBG14903 [Caenorhabditis briggsae] 5.0E-75
Protein Domains
Database ID Description E-Value
scop a.2.7.Phenylalanyl-tRNA synthetase (PheRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Phenylalanyl-tRNA synthetase (PheRS) 2.40000009536743
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-23
Sequence
Target Sequence 
>C. ELEGANS:189249_AT
ggagagcgctacgattgcagtacttgaagaggcattccgtccaaaaacatccgacgttcc
cggaggtgaccagtacgtggtgaagcctcttttgaagctgtcatggggatcggtcgttgc
tgcatctgattttccaactgcggatgaggtagaaacagttgaagagaaagatctgaaaaa
gaagcaagacgccaagaatcaagaatctgataaaagaaagaactcggatcgaaaaaagaa
aagtgagagaaagaagaaaggatcgaaaggagacatgattctggagaagtcgaaagaaac
gaaggatcatagagaaagtggacgtcgcctcactgatcgggaagctaatgctgttggcgg
aaaatccatcgatgccaagtcaaagggaaataaggatatggagcaaaggactatcatgat
tgacaaacctggaagcataactccgaagagcaaacggtcgagaacaacaactaaaattcc
acataaaactccacataaatctcatctcagaaaggagaagcaggagtcgtcagccaactc
gaagagattgaatgttgccccattggttcatcccacagtgcttgtagtcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGAGCGCTACGATTGCAGTACTT 236 519 1044 Antisense
AGGCATTCCGTCCAAAAACATCCGA 526 53 1073 Antisense
GTTCCCGGAGGTGACCAGTACGTGG 300 437 1099 Antisense
GGTGAAGCCTCTTTTGAAGCTGTCA 185 501 1122 Antisense
TGTCATGGGGATCGGTCGTTGCTGC 289 559 1142 Antisense
GCTGCATCTGATTTTCCAACTGCGG 222 297 1162 Antisense
AGAGAAAGTGGACGTCGCCTCACTG 674 67 1354 Antisense
TCGCCTCACTGATCGGGAAGCTAAT 312 597 1368 Antisense
AAAACTCCACATAAATCTCATCTCA 447 131 1528 Antisense
TTGAATGTTGCCCCATTGGTTCATC 343 705 1591 Antisense
GTTCATCCCACAGTGCTTGTAGTCC 342 445 1609 Antisense
Sequence Source Affymetrix Proprietary Database