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[189248_at] forkhead domain

Description T27A8.2 /REP_DB=WormBase Gene ID /WP=CE03742 /TR=Q22826 /GB=CAA92222.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=forkhead domain
Cluster 6grp_total > 67      4grp_total > 25      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 5.2
(-0.6)
A 		6.9
(3)
A 		4.1
(-3)
A 		6.8
(2.8)
A 		3
B 3
(-3)
A 		4.4
(-1.8)
A 		9.9
(3)
A 		3.6
(-2.5)
A 		7
C 3.3
(-2.9)
A 		8.2
(-0.8)
A 		3
(-3)
A 		16.9
(3)
A 		14
avr 3.8
(-3) 		6.5
(0.1) 		5.7
(-0.8) 		9.1
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 2.7
(-3)
A 		3
(-2.9)
A 		3.4
(-2.8)
A 		2.9
(-2.9)
A 		3.7
(-2.7)
A 		24
(3)
A 		21
B 3.7
(-2.6)
A 		1.8
(-3)
A 		3.9
(-2.6)
A 		1.7
(-3)
A 		5.4
(-2.3)
A 		32.1
(3)
A 		30
C 1.9
(-3)
A 		2.1
(-3)
A 		3
(-2.9)
A 		4.4
(-2.8)
A 		5.9
(-2.6)
A 		62
(3)
A 		60
avr 2.8
(-2.9) 		2.3
(-3) 		3.4
(-2.8) 		3
(-2.9) 		5
(-2.6) 		39.4
(3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 1.9
(3)
A 		1.5
(0.3)
A 		1.1
(-2.3)
A 		1
(-3)
A 		1
B 1
(-2.2)
A 		1.1
(-1.8)
A 		2.3
(3)
A 		0.8
(-3)
A 		2
C 1.1
(-2.7)
A 		2.4
(1.4)
A 		1
(-3)
A 		2.9
(3)
A 		2
avr 1.3
(-3) 		1.7
(3) 		1.5
(0) 		1.6
(1.5) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 0.5
(-3)
A 		1.1
(-2.3)
A 		0.8
(-2.7)
A 		0.7
(-2.8)
A 		1.1
(-2.3)
A 		5.8
(3)
A 		5
B 1.3
(-2.6)
A 		0.6
(-2.9)
A 		0.9
(-2.8)
A 		0.5
(-3)
A 		2.2
(-2.1)
A 		12
(3)
A 		12
C 0.7
(-3)
A 		0.6
(-3)
A 		0.8
(-2.9)
A 		0.9
(-2.9)
A 		1.9
(-2.6)
A 		20.8
(3)
A 		20
avr 0.8
(-3) 		0.8
(-3) 		0.8
(-3) 		0.7
(-3) 		1.7
(-2.5) 		12.9
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189248_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17556
Sequence Type Exemplar sequence
Representative Public ID T27A8.2 NCBI
Target Description T27A8.2 /REP_DB=WormBase Gene ID /WP=CE03742 /TR=Q22826 /GB=CAA92222.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=forkhead domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078225(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078225 NCBI Caenorhabditis elegans T27A8.2 (T27A8.2) mRNA, complete cds. 11/11 None
SNAP00000006723 ENSEMBL cdna:SNAP chromosome:CEL140:X:16057745:16058827:-1 11/11 None
GENEFINDER00000006729 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16057745:16058848:-1 11/11 None
T27A8.2 ENSEMBL cdna:known chromosome:CEL140:X:16057745:16058848:-1 gene:T27A8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16057744-16058848(-) 100.0 100.0
Public Domain and Genome References
Gene Title forkhead domain
Ensembl T27A8.2
Entrez Gene 188967 Entrez gene
SwissProt Q22826 EMBL-EBI
WormBase CE03742 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92222 Hypothetical protein T27A8.2 [Caenorhabditis elegans] ref|NP_510626.1| T27A8.2 [Caenorhabditis elegans] 5.0E-80
blast CAA92222 Hypothetical protein T27A8.2 [Caenorhabditis elegans] ref|NP_510626.1| T27A8.2 [Caenorhabditis elegans] 3.0E-75
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 0.0829999968409538
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 0.0900000035762787
Sequence
Target Sequence 
>C. ELEGANS:189248_AT
tatggggcttcatcatccggaaatggcgatgcccaagttcaaaatggtcgccctggtcag
tgctgcgatggaagaagcggatgacgtgtttccggagattgaagtagttgtcaagtggat
caccgacttgttcccgtattacaaagtgtttcacgatatgtacaaatgggagtttgttaa
tcatgtggcctgggttctggacaccgaactgtgctttcgtcgagaaagagacgtgagatc
tggactatgggcttatgaagacggcaacaaggatattctggatgttgaggaggagtttcg
aaaggatatagacgagttgaagcaatatgtggagcatcaggtggaaatgacggatcttgc
tgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGGGGCTTCATCATCCGGAAATG 309 653 45 Antisense
GGAAATGGCGATGCCCAAGTTCAAA 196 527 63 Antisense
AAGTTCAAAATGGTCGCCCTGGTCA 331 151 79 Antisense
CCCTGGTCAGTGCTGCGATGGAAGA 222 259 95 Antisense
GAAGCGGATGACGTGTTTCCGGAGA 38 343 118 Antisense
CGACTTGTTCCCGTATTACAAAGTG 350 245 168 Antisense
TGTTAATCATGTGGCCTGGGTTCTG 407 561 219 Antisense
CCTGGGTTCTGGACACCGAACTGTG 127 265 233 Antisense
ACACCGAACTGTGCTTTCGTCGAGA 572 109 245 Antisense
GACGTGAGATCTGGACTATGGGCTT 620 377 274 Antisense
GGTGGAAATGACGGATCTTGCTGGA 268 499 384 Antisense
Sequence Source Affymetrix Proprietary Database