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[189243_at]

Description F08C6.1 /REP_DB=WormBase Gene ID /WP=CE27923 /TR=Q19204 /GB=AAA68721.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Thrombospondin
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 12      4grp_high_8_8 > 42      6grp_highest_nonorm > 70      6grp_total > 23      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 344
1 2 3 4 range
A 425.7
(3)
P 		294.9
(0.5)
P 		243.7
(-0.5)
P 		117.5
(-3)
A 		308
B 446.6
(3)
P 		284.7
(0)
P 		248.3
(-0.6)
P 		120
(-3)
P 		327
C 357.6
(3)
P 		312.2
(1.9)
P 		249
(0.4)
P 		102.6
(-3)
P 		255
avr 410
(3) 		297.3
(0.7) 		247
(-0.3) 		113.4
(-3) 		297

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 305
1 2 3 4 5 6 range
A 286.4
(0.6)
P 		389.8
(3)
P 		279.3
(0.5)
P 		305.8
(1.1)
P 		182.7
(-1.8)
P 		128.8
(-3)
P 		261
B 311.2
(2.9)
P 		312.9
(3)
P 		298.1
(2.5)
P 		211.2
(-0.2)
P 		188.3
(-0.9)
P 		120.7
(-3)
P 		192
C 425.5
(3)
P 		303.4
(0.6)
P 		367.3
(1.8)
P 		267.7
(-0.1)
P 		214.1
(-1.2)
P 		124.1
(-3)
P 		301
avr 341
(3) 		335.4
(2.8) 		314.9
(2.3) 		261.6
(0.8) 		195
(-1) 		124.5
(-3) 		217
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 155
(3)
P 		64
(-1)
P 		63.7
(-1)
P 		17.2
(-3)
A 		138
B 142.4
(3)
P 		70.3
(-0.7)
P 		57.5
(-1.4)
P 		25.6
(-3)
P 		117
C 118.4
(3)
P 		89.8
(1.3)
P 		86.8
(1.1)
P 		17.3
(-3)
P 		101
avr 138.6
(3) 		74.7
(-0.2) 		69.3
(-0.5) 		20
(-3) 		119

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 52.8
(-1.8)
P 		138.6
(3)
P 		60.8
(-1.4)
P 		77.6
(-0.4)
P 		55.1
(-1.7)
P 		31.4
(-3)
P 		107
B 108.3
(3)
P 		104.2
(2.6)
P 		72.4
(-0.4)
P 		55.9
(-2)
P 		76.5
(-0)
P 		45
(-3)
P 		63
C 157.7
(3)
P 		92.6
(-0.4)
P 		93.5
(-0.3)
P 		53.8
(-2.4)
P 		69.3
(-1.6)
P 		41.5
(-3)
P 		116
avr 106.3
(2.5) 		111.8
(3) 		75.6
(0) 		62.4
(-1.1) 		67
(-0.7) 		39.3
(-3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189243_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16973
Sequence Type Exemplar sequence
Representative Public ID F08C6.1 NCBI
Target Description F08C6.1 /REP_DB=WormBase Gene ID /WP=CE27923 /TR=Q19204 /GB=AAA68721.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Thrombospondin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029361(11), NM_001029363(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029361 NCBI Caenorhabditis elegans ADAMTS family member (adt-2) (adt-2) mRNA, complete cds. 11/11 None
NM_001029363 NCBI Caenorhabditis elegans ADAMTS family member (adt-2) (adt-2) mRNA, complete cds. 9/11 None
SNAP00000044582 ENSEMBL cdna:SNAP chromosome:CEL140:X:7591155:7593756:1 9/11 None
F08C6.1a.2 ENSEMBL cdna:known chromosome:CEL140:X:7585529:7595281:1 gene:F08C6.1 11/11 None
F08C6.1a.1 ENSEMBL cdna:known chromosome:CEL140:X:7586052:7595331:1 gene:F08C6.1 11/11 None
F08C6.1c ENSEMBL cdna:known chromosome:CEL140:X:7586151:7593756:1 gene:F08C6.1 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029362 7/11 Cross Hyb Matching Probes None
SNAP00000016787 2/11 Cross Hyb Matching Probes None
F08C6.1b 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181022 3 2 NM_001029361 174723_at (11), 189243_at (11)
NM_001029363 189243_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7588364-7594734(+) 99.2 100.0
Public Domain and Genome References
Ensembl F08C6.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68721 Adamts family protein 2, isoform a [Caenorhabditis elegans] ref|NP_001024532.1| ADAMTS family member (adt-2) [Caenorhabditis elegans] 0.0
blast BAC41253.1 ADAMTS-like protease [Caenorhabditis elegans] 0.0
blast AAP40541 Adamts family protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024534.1| ADAMTS family member (adt-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001590 EMBL-EBI Peptidase M12B, ADAM/reprolysin 8.6E-9
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 3.5E-12
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 1.4E-13
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 9.5E-9
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 1.7E-10
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 3.4E-11
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 7.6E-13
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 3.5E-12
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 2.5E-10
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 9.5E-9
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 1.7E-10
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 3.4E-11
Sequence
Target Sequence 
>C. ELEGANS:189243_AT
acatggggaggttggagcacttgctcaacatcttgtggacctggtactctcgtcagacaa
cgaacttgcaatagagagccatgtgatgggtcagctcacgaacgtagatcttgcaacgta
gcaacatgtcaaaacgatggaatttggtcgctatggaatgaatggtctgactgcagcaga
gtttgcggaaaaggactcagaagtagaagcaggtcatgttttggaagtggatgtatgggt
gcaagttctgaacaacaattctgcaacgaacaagcatgtgcttcgtcaagtgcaaacgat
tggggtacctggtcgggatggtcgcagtgctcagtgagttgcggtgcaggagtgaagagg
aggacgaggacgtgtcgcactggaaattgtcctggaaattacaaagaatcagcgatttgc
aacgatcgagattgtgaaaacaaaaatgctgcatggggaggatggggatactggtcatcg
tgtagtgaaacatgtggcgacggtgttcgtaaacgtgtaagaaagtgctatggaagtggt
aactgcgacggtcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATGGGGAGGTTGGAGCACTTGCT 130 107 2275 Antisense
GAGCACTTGCTCAACATCTTGTGGA 5 391 2289 Antisense
CATCTTGTGGACCTGGTACTCTCGT 490 213 2303 Antisense
TGATGGGTCAGCTCACGAACGTAGA 320 567 2358 Antisense
GCATGTGCTTCGTCAAGTGCAAACG 211 311 2548 Antisense
GATTGGGGTACCTGGTCGGGATGGT 68 239 2572 Antisense
TCGGGATGGTCGCAGTGCTCAGTGA 638 605 2587 Antisense
GTGCTCAGTGAGTTGCGGTGCAGGA 88 481 2601 Antisense
GACGAGGACGTGTCGCACTGGAAAT 156 379 2637 Antisense
GTGGCGACGGTGTTCGTAAACGTGT 498 433 2768 Antisense
GGAAGTGGTAACTGCGACGGTCAAC 399 525 2806 Antisense
Sequence Source Affymetrix Proprietary Database