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[189233_s_at]

Description C44C8.6 /REP_DB=WormBase Gene ID /WP=CE17545 /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase (ST.LOUIS) TR:Q9TZ16 protein_id:AAC68944.1
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 18      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 20      6grp_total > 28      4grp_total > 02      6grp_highest_sub > De      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4821
1 2 3 4 range
A 884.7
(-2.5)
P 		869.1
(-2.5)
P 		537.8
(-3)
P 		4451.9
(3)
P 		3914
B 979.4
(-2.5)
P 		672.7
(-2.9)
P 		620.6
(-3)
P 		5359.2
(3)
P 		4739
C 959.9
(-2.5)
P 		644.7
(-3)
P 		623.8
(-3)
P 		4364
(3)
P 		3740
avr 941.3
(-2.5) 		728.8
(-2.8) 		594.1
(-3) 		4725
(3) 		4131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 930
1 2 3 4 5 6 range
A 1517.2
(3)
P 		1014.3
(-0.4)
P 		653
(-2.9)
P 		641.8
(-3)
P 		881.9
(-1.4)
P 		700.6
(-2.6)
P 		875
B 1438.5
(3)
P 		1055.3
(-0.7)
P 		927.3
(-1.9)
P 		835.4
(-2.8)
P 		946.1
(-1.7)
P 		816.1
(-3)
P 		622
C 1290.3
(3)
P 		1082.6
(1.2)
P 		708.4
(-2)
P 		586.8
(-3)
P 		834.8
(-0.9)
P 		911.8
(-0.2)
P 		704
avr 1415.3
(3) 		1050.7
(-0) 		762.9
(-2.4) 		688
(-3) 		887.6
(-1.4) 		809.5
(-2) 		727
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1001
1 2 3 4 range
A 322.1
(-0.9)
P 		188.7
(-2.4)
P 		140.6
(-3)
P 		651.7
(3)
P 		511
B 312.3
(-2)
P 		166.1
(-2.9)
P 		143.8
(-3)
P 		1142
(3)
P 		998
C 317.8
(-1.6)
P 		185.4
(-3)
P 		217.4
(-2.7)
P 		735.8
(3)
P 		550
avr 317.4
(-1.7) 		180.1
(-2.9) 		167.3
(-3) 		843.2
(3) 		676

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 383
1 2 3 4 5 6 range
A 279.7
(0.8)
P 		360.7
(3)
P 		142.2
(-3)
P 		162.8
(-2.4)
P 		266.1
(0.4)
P 		170.6
(-2.2)
P 		219
B 500.5
(3)
P 		351.3
(-0.2)
P 		225.2
(-2.9)
P 		221.3
(-3)
P 		384.4
(0.5)
P 		304.3
(-1.2)
P 		279
C 478.3
(3)
P 		330.3
(0.5)
P 		180.3
(-2)
P 		118
(-3)
P 		270.1
(-0.5)
P 		305.2
(0.1)
P 		360
avr 419.5
(3) 		347.4
(1.3) 		182.6
(-2.6) 		167.4
(-3) 		306.9
(0.3) 		260
(-0.8) 		252
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189233_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10579
Sequence Type Exemplar sequence
Representative Public ID C44C8.6 NCBI
Target Description C44C8.6 /REP_DB=WormBase Gene ID /WP=CE17545 /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase (ST.LOUIS) TR:Q9TZ16 protein_id:AAC68944.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067604(11), NM_067605(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067604 NCBI Caenorhabditis elegans MAP kinase Activated protein Kinase family member (mak-2) (mak-2) mRNA, complete cds. 11/11 None
NM_067605 NCBI Caenorhabditis elegans MAP kinase Activated protein Kinase family member (mak-2) (mak-2) mRNA, complete cds. 11/11 None
SNAP00000021734 ENSEMBL cdna:SNAP chromosome:CEL140:IV:879793:889894:1 11/11 None
GENEFINDER00000021754 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:887145:889894:1 11/11 None
C44C8.6a ENSEMBL cdna:known chromosome:CEL140:IV:884909:890208:1 gene:C44C8.6 11/11 None
C44C8.6b.1 ENSEMBL cdna:known chromosome:CEL140:IV:885344:889894:1 gene:C44C8.6 11/11 None
C44C8.6b.2 ENSEMBL cdna:known chromosome:CEL140:IV:885346:889894:1 gene:C44C8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:884914-889900(+) 100.0 100.0
Public Domain and Genome References
Ensembl C44C8.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68944 Map kinase activated protein kinase protein 2, isoform a [Caenorhabditis elegans] ref|NP_500005.1| MAP kinase Activated protein Kinase family member (mak-2) [Caenorhabditis elegans] 0.0
blast CAE68292 Hypothetical protein CBG13977 [Caenorhabditis briggsae] 0.0
blast AAK68684 Map kinase activated protein kinase protein 2, isoform b [Caenorhabditis elegans] ref|NP_500006.2| MAP kinase Activated protein Kinase family member (mak-2) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 8.0E-60
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-60
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-60
hanks 2.3.1 CaMK Group; CaMK Other; MAPKAP2 1.0E-91
hanks 2.3.1 CaMK Group; CaMK Other; MAPKAP2 7.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.5E-75
Pfam IPR000719 EMBL-EBI Protein kinase 2.5E-75
Pfam IPR000719 EMBL-EBI Protein kinase 2.0E-74
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.1E-28
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-77
Sequence
Target Sequence 
>C. ELEGANS:189233_S_AT
acagatgaatcggaaccccaaggcctaaaaacggcgtgcttcaccccttactattgtgct
ccggaagtgctcggcaccgaaaaatatgataaatcttgtgatttatggtcgatcggagtt
attatgtatattttactgtgcggctacccaccattttactctcaacacggacaaccaatg
tcacctggaatgaaggcgaaaatcaagtcaggacagtacacatttccgagtcccgaatgg
gattgtgtttctgaagcagccaaagacctaatccgaaagcttctccgaactgagcccact
gaacgtatcacaattgagcagacaatggagcacaagtggatatctcattaccgtagagtt
ccagatactccattattcacaagttcaaatctgaacgatcaacgggaacaatggacggat
atgcaggatgaaatggaggctacattggcctcaatgagagttggtcctgaaaatattcag
attaaaagccttggagactcgaataataagcttttggcgaagaggagaaaaggtggagct
agtccgaaagacgatcccatggacgatatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGATGAATCGGAACCCCAAGGCC 542 105 730 Antisense
ATTGTGCTCCGGAAGTGCTCGGCAC 516 3 782 Antisense
ACACGGACAACCAATGTCACCTGGA 290 109 894 Antisense
GGACAGTACACATTTCCGAGTCCCG 499 525 940 Antisense
GTCCCGAATGGGATTGTGTTTCTGA 254 475 959 Antisense
TAATCCGAAAGCTTCTCCGAACTGA 109 637 998 Antisense
CGAACTGAGCCCACTGAACGTATCA 406 253 1015 Antisense
ACAAGTGGATATCTCATTACCGTAG 91 111 1061 Antisense
AGTTCCAGATACTCCATTATTCACA 100 65 1086 Antisense
TGGCCTCAATGAGAGTTGGTCCTGA 332 545 1175 Antisense
AAGACGATCCCATGGACGATATCAA 273 153 1277 Antisense
Sequence Source Affymetrix Proprietary Database