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[189230_s_at]

Description ZC123.4 /REP_DB=WormBase Gene ID /WP=CE17302 /TR=O45022 /GB=AAB97606.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 12      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 70      6grp_total > 23      4grp_total > 37      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 341
1 2 3 4 range
A 578
(3)
P 		444.1
(0.3)
P 		283.6
(-3)
P 		343.9
(-1.8)
P 		294
B 464.2
(3)
P 		409.1
(1.1)
P 		292.3
(-3)
P 		347.6
(-1.1)
P 		172
C 473.8
(2.8)
P 		481
(3)
P 		236.8
(-3)
P 		302.1
(-1.4)
P 		244
avr 505.3
(3) 		444.7
(1.4) 		270.9
(-3) 		331.2
(-1.5) 		234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 300.1
(1.5)
P 		343.4
(3)
P 		293.4
(1.3)
P 		242.9
(-0.5)
P 		211.2
(-1.6)
P 		171.6
(-3)
P 		172
B 338.1
(3)
P 		302.9
(1.6)
P 		326
(2.5)
P 		255.1
(-0.4)
P 		208.7
(-2.2)
P 		190
(-3)
P 		148
C 415.6
(3)
P 		341.4
(1.2)
P 		313.3
(0.5)
P 		218.3
(-1.8)
P 		211
(-2)
P 		168.1
(-3)
P 		248
avr 351.3
(3) 		329.2
(2.2) 		310.9
(1.6) 		238.8
(-0.9) 		210.3
(-1.8) 		176.6
(-3) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 210.5
(3)
P 		96.4
(-1.3)
P 		74.1
(-2.1)
P 		50.3
(-3)
P 		160
B 148
(3)
P 		101
(-0.5)
P 		67.7
(-3)
P 		74.1
(-2.5)
P 		80
C 156.8
(3)
P 		138.3
(2)
P 		82.5
(-1.2)
P 		50.9
(-3)
P 		106
avr 171.8
(3) 		111.9
(-0.2) 		74.8
(-2.1) 		58.4
(-3) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 55.3
(-2)
P 		122.1
(3)
P 		63.9
(-1.3)
P 		61.6
(-1.5)
P 		63.7
(-1.4)
P 		41.8
(-3)
P 		80
B 117.6
(3)
P 		100.8
(1)
P 		79.2
(-1.6)
P 		67.6
(-3)
P 		84.8
(-0.9)
P 		70.8
(-2.6)
P 		50
C 154.1
(3)
P 		104.2
(0.3)
P 		79.7
(-1.1)
P 		43.9
(-3)
P 		68.3
(-1.7)
P 		56.3
(-2.3)
P 		110
avr 109
(3) 		109
(3) 		74.3
(-1) 		57.7
(-2.8) 		72.3
(-1.2) 		56.3
(-3) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189230_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18
Sequence Type Exemplar sequence
Representative Public ID ZC123.4 NCBI
Target Description ZC123.4 /REP_DB=WormBase Gene ID /WP=CE17302 /TR=O45022 /GB=AAB97606.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026671(11), NM_058382(11), NM_182008(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026671 NCBI Caenorhabditis elegans ZC123.4c (ZC123.4) mRNA, complete cds. 11/11 None
NM_058382 NCBI Caenorhabditis elegans ZC123.4a (ZC123.4) mRNA, complete cds. 11/11 None
NM_182008 NCBI Caenorhabditis elegans ZC123.4b (ZC123.4) mRNA, complete cds. 9/11 None
ZC123.4b ENSEMBL cdna:known chromosome:CEL140:I:841753:847613:-1 gene:ZC123.4 10/11 None
ZC123.4c ENSEMBL cdna:known chromosome:CEL140:I:841756:846765:-1 gene:ZC123.4 11/11 None
ZC123.4a ENSEMBL cdna:known chromosome:CEL140:I:841838:847613:-1 gene:ZC123.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:841837-844301(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZC123.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU87803 Hypothetical protein ZC123.4c [Caenorhabditis elegans] ref|NP_001021842.1| ZC123.4c [Caenorhabditis elegans] 0.0
blast AAB97606 Hypothetical protein ZC123.4a [Caenorhabditis elegans] ref|NP_490783.2| ZC123.4a [Caenorhabditis elegans] 0.0
blast CAE71594 Hypothetical protein CBG18552 [Caenorhabditis briggsae] 0.0
blast AAU87802 Hypothetical protein ZC123.4b [Caenorhabditis elegans] ref|NP_871808.2| ZC123.4b [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 9.0E-82
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-85
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 5.0E-71
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 5.0E-76
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 2.0E-80
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 1.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-80
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-87
Pfam IPR000719 EMBL-EBI Protein kinase 8.6E-72
Sequence
Target Sequence 
>C. ELEGANS:189230_S_AT
cagactttggtctagccagggcgaaatctgtgccgagtaggacatattctcacgaggtgg
tcactttatggtaccgcccgcctgatgttctcatgggaagtaccgactattcaacttccc
tggatatgtggggagtcggttgcatattcgctgaaatttgcacgggcgccgctttgttcc
cgggatccaaggactctcactaccccggcaccaaggaccagttggatatgatcttcagca
ttcggggtactccagacgagaaaaagtggccggaggtaaagacattaccaggctacaccc
ctgaactgttccccaggtatcgggagttgtcgttcatcgcggtgaacccgatgttcacga
agattctgaagactggtcaggaacttctggggatgcttcttcagctccgcccggaaagcc
gtgtctccgcagccagtgcaatgcttcacccctactttgcctcattcccccgcgaggttc
atctcctggctccaagccagtcaatattccggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGACTTTGGTCTAGCCAGGGCGAA 431 193 605 Antisense
GAGGTGGTCACTTTATGGTACCGCC 321 401 658 Antisense
GGGAGTCGGTTGCATATTCGCTGAA 368 495 735 Antisense
TCGCTGAAATTTGCACGGGCGCCGC 443 599 752 Antisense
TTTGTTCCCGGGATCCAAGGACTCT 649 627 777 Antisense
TCAGCATTCGGGGTACTCCAGACGA 46 601 839 Antisense
TAAAGACATTACCAGGCTACACCCC 472 639 881 Antisense
CCTGAACTGTTCCCCAGGTATCGGG 662 263 904 Antisense
GGTATCGGGAGTTGTCGTTCATCGC 111 505 920 Antisense
GAACTTCTGGGGATGCTTCTTCAGC 172 351 985 Antisense
GCTCCAAGCCAGTCAATATTCCGGC 674 297 1093 Antisense
Sequence Source Affymetrix Proprietary Database