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[189227_at] thiol protease

Description W07B8.1 /REP_DB=WormBase Gene ID /WP=CE14674 /TR=O16289 /GB=AAB65343.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=thiol protease
Cluster 6grp_high1_10_10 > 07      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_total > 08      4grp_total > 57      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1009
1 2 3 4 range
A 398.8
(-1.9)
P 		735.9
(1.7)
P 		856.7
(3)
P 		293.2
(-3)
P 		564
B 315
(-3)
P 		904.4
(0.6)
P 		1302.3
(3)
P 		319
(-3)
P 		987
C 442
(-1.9)
P 		772.7
(1.2)
P 		975.3
(3)
P 		316.1
(-3)
P 		659
avr 385.3
(-2.4) 		804.3
(1) 		1044.8
(3) 		309.4
(-3) 		735

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 389
1 2 3 4 5 6 range
A 403.8
(3)
P 		202.5
(-0.9)
P 		192
(-1.1)
P 		189.7
(-1.2)
P 		136.9
(-2.2)
P 		95.7
(-3)
A 		308
B 484.3
(3)
P 		185.2
(-1.7)
P 		167.5
(-2)
P 		131.6
(-2.5)
P 		119.7
(-2.7)
P 		101.6
(-3)
P 		383
C 483.4
(3)
P 		242
(-1.1)
P 		241.7
(-1.1)
P 		167.4
(-2.3)
P 		136.1
(-2.8)
P 		126.3
(-3)
P 		357
avr 457.2
(3) 		209.9
(-1.2) 		200.4
(-1.4) 		162.9
(-2.1) 		130.9
(-2.6) 		107.9
(-3) 		349
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 297
1 2 3 4 range
A 145.2
(0.4)
P 		159.7
(0.9)
P 		224
(3)
P 		42.9
(-3)
P 		181
B 100.4
(-2.2)
P 		223.4
(1)
P 		301.8
(3)
P 		68
(-3)
P 		234
C 146.3
(-1.1)
P 		222.2
(0.5)
P 		339.9
(3)
P 		53.3
(-3)
P 		287
avr 130.6
(-1.1) 		201.8
(0.8) 		288.6
(3) 		54.7
(-3) 		234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 156
1 2 3 4 5 6 range
A 74.4
(3)
P 		72
(2.7)
P 		41.8
(-0.8)
P 		48.1
(-0.1)
P 		41.3
(-0.9)
P 		23.3
(-3)
A 		51
B 168.5
(3)
P 		61.6
(-1.8)
P 		40.7
(-2.7)
P 		34.9
(-3)
P 		48.6
(-2.4)
P 		37.9
(-2.9)
P 		134
C 179.2
(3)
P 		73.8
(-1.3)
P 		61.5
(-1.9)
P 		33.7
(-3)
P 		44
(-2.6)
P 		42.3
(-2.6)
P 		146
avr 140.7
(3) 		69.1
(-1) 		48
(-2.2) 		38.9
(-2.8) 		44.6
(-2.4) 		34.5
(-3) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189227_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15400
Sequence Type Exemplar sequence
Representative Public ID W07B8.1 NCBI
Target Description W07B8.1 /REP_DB=WormBase Gene ID /WP=CE14674 /TR=O16289 /GB=AAB65343.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=thiol protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070983(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070983 NCBI Caenorhabditis elegans W07B8.1 (W07B8.1) mRNA, complete cds. 11/11 None
SNAP00000016193 ENSEMBL cdna:SNAP chromosome:CEL140:V:1132258:1136762:1 10/11 None
GENEFINDER00000016196 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1134456:1136762:1 10/11 None
W07B8.1 ENSEMBL cdna:known chromosome:CEL140:V:1134456:1138979:1 gene:W07B8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1134455-1138950(+) 100.0 100.0
Public Domain and Genome References
Gene Title thiol protease
Ensembl W07B8.1
Entrez Gene 178613 Entrez gene
SwissProt O16289 EMBL-EBI
WormBase CE14674 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8234 cysteine-type peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65343 Hypothetical protein W07B8.1 [Caenorhabditis elegans] ref|NP_503384.1| W07B8.1 [Caenorhabditis elegans] 0.0
blast AAB65343 Hypothetical protein W07B8.1 [Caenorhabditis elegans] ref|NP_503384.1| W07B8.1 [Caenorhabditis elegans] 1.0E-175
blast CAE58028 Hypothetical protein CBG01102 [Caenorhabditis briggsae] 1.0E-140
blast CAE58028 Hypothetical protein CBG01102 [Caenorhabditis briggsae] 1.0E-125
blast AAB65346 Cysteine protease related protein 5 [Caenorhabditis elegans] ref|NP_503383.1| Cysteine PRotease related family member (cpr-5) [Caenorhabditis elegans] sp|P43509|CPR5_CAEEL Cathepsin B-like cysteine proteinase 5 precursor (Cysteine protease-related 5) gb|AAA98784.1| cathepsin B-like cysteine proteinase gb|AAA98786.1| cathepsin B-like cysteine proteinase [Caenorhabditis elegans] 2.0E-95
Protein Domains
Database ID Description E-Value
Pfam IPR000668 EMBL-EBI Peptidase C1A, papain 1.7E-102
Pfam IPR000668 EMBL-EBI Peptidase C1A, papain 2.7E-90
Pfam IPR000668 EMBL-EBI Peptidase C1A, papain 2.7E-90
Sequence
Target Sequence 
>C. ELEGANS:189227_AT
ggcgggaacccattcaaagcttggcagtatattcagaaacacggtattcctaccggagga
tcctatgaatcgcagtttggatgtaaaccttactctatacctccatgcggaaaaactgtt
ggcaatgtgacctacccagcgtgtacaaatactacatcacctactccgagttgtgagaaa
aagtgtacttcacgaatcggatacccaattgatattgataaagatcggcattatggagtt
tctgtggatcaacttccaaatagtcaaatagaaattcaaagtgacgtcatgctaaatggg
ccgatccaggcaacttttgaggtttacgacgattttcttcaatatacgacgggaatttat
gtgcatttgactggcaacaaacagggtcacttgtcagttagaattattggctggggagtt
tggcaaggtgttccttattggctatgcgcaaactcttggggaaggcagtggggagagaat
ggaactttccgtgttctacgtgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCGGGAACCCATTCAAAGCTTGGC 305 539 460 Antisense
GGTATTCCTACCGGAGGATCCTATG 505 505 502 Antisense
CTTACTCTATACCTCCATGCGGAAA 519 221 548 Antisense
CTGTTGGCAATGTGACCTACCCAGC 386 237 575 Antisense
CTACATCACCTACTCCGAGTTGTGA 381 227 611 Antisense
GTGTACTTCACGAATCGGATACCCA 68 485 642 Antisense
GACGTCATGCTAAATGGGCCGATCC 77 379 742 Antisense
GGGCCGATCCAGGCAACTTTTGAGG 131 493 757 Antisense
AGGTGTTCCTTATTGGCTATGCGCA 425 57 885 Antisense
GGCTATGCGCAAACTCTTGGGGAAG 572 535 899 Antisense
TGGAACTTTCCGTGTTCTACGTGGA 103 547 939 Antisense
Sequence Source Affymetrix Proprietary Database