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[189221_at] cytochrome P450

Description F28G4.1 /REP_DB=WormBase Gene ID /WP=CE15919 /TR=O17851 /GB=CAB07604.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 15      4grp_high_8_8 > 06      6grp_highest_nonorm > 97      6grp_total > 85      4grp_total > 10      6grp_highest_sub > In      6grp_cgc > 77      6grp_high1_sub > In      6grp_cgc_sub > 04_05_06      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1993
1 2 3 4 range
A 253.4
(-3)
P 		356.7
(-2.7)
P 		315.7
(-2.8)
P 		2164.8
(3)
P 		1911
B 218.6
(-3)
P 		347.8
(-2.5)
P 		244.7
(-2.9)
P 		1845
(3)
P 		1626
C 263.4
(-3)
P 		328.1
(-2.8)
P 		274
(-3)
P 		2211.2
(3)
P 		1948
avr 245.1
(-3) 		344.2
(-2.7) 		278.1
(-2.9) 		2073.7
(3) 		1829

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 906
1 2 3 4 5 6 range
A 503.9
(-3)
P 		634.8
(-2)
P 		967.7
(0.5)
P 		1103.4
(1.6)
P 		1225.3
(2.5)
P 		1289.7
(3)
P 		786
B 496.8
(-3)
P 		587.4
(-2.3)
P 		725
(-1.2)
P 		1175.6
(2.3)
P 		1060.1
(1.4)
P 		1267.5
(3)
P 		771
C 452.1
(-3)
P 		647.5
(-1.7)
P 		987.4
(0.5)
P 		1358.5
(3)
P 		1130.3
(1.5)
P 		1321.8
(2.8)
P 		906
avr 484.3
(-3) 		623.2
(-2) 		893.4
(0) 		1212.5
(2.4) 		1138.6
(1.9) 		1293
(3) 		809
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 337
1 2 3 4 range
A 92.3
(-2.6)
P 		77.4
(-3)
P 		82.5
(-2.9)
P 		316.9
(3)
P 		240
B 69.7
(-2.8)
P 		85.9
(-2.5)
P 		56.7
(-3)
P 		393.2
(3)
P 		337
C 87.2
(-3)
P 		94.3
(-2.9)
P 		95.5
(-2.8)
P 		372.8
(3)
P 		286
avr 83.1
(-2.9) 		85.9
(-2.8) 		78.2
(-3) 		361
(3) 		283

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 380
1 2 3 4 5 6 range
A 92.9
(-3)
P 		225.8
(-0.1)
P 		210.7
(-0.4)
P 		279.9
(1.1)
P 		369.7
(3)
P 		314
(1.8)
P 		277
B 172.9
(-3)
P 		195.5
(-2.5)
P 		176
(-2.9)
P 		311.4
(-0.2)
P 		430.7
(2.2)
P 		472.7
(3)
P 		300
C 167.6
(-3)
P 		197.6
(-2.3)
P 		251.3
(-1.2)
P 		273.2
(-0.7)
P 		365.7
(1.3)
P 		442.5
(3)
P 		275
avr 144.5
(-3) 		206.3
(-1.6) 		212.7
(-1.5) 		288.2
(0.3) 		388.7
(2.5) 		409.7
(3) 		265
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189221_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12571
Sequence Type Exemplar sequence
Representative Public ID F28G4.1 NCBI
Target Description F28G4.1 /REP_DB=WormBase Gene ID /WP=CE15919 /TR=O17851 /GB=CAB07604.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074708(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074708 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-37B1) (cyp-37B1) mRNA, complete cds. 11/11 None
SNAP00000032993 ENSEMBL cdna:SNAP chromosome:CEL140:V:16281047:16283143:1 11/11 None
GENEFINDER00000033001 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16281047:16283143:1 11/11 None
F28G4.1 ENSEMBL cdna:known chromosome:CEL140:V:16280774:16283195:1 gene:F28G4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16280848-16283143(+) 99.93 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-37B1
Ensembl F28G4.1
Entrez Gene 180093 Entrez gene
SwissProt O17851 EMBL-EBI
WormBase CE15919 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258111_AT cytochrome P450, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07604 Hypothetical protein F28G4.1 [Caenorhabditis elegans] ref|NP_507109.1| CYtochrome P450 family member (cyp-37B1) [Caenorhabditis elegans] 0.0
blast CAE69638 Hypothetical protein CBG15879 [Caenorhabditis briggsae] 0.0
blast CAB04044 Hypothetical protein F01D5.9 [Caenorhabditis elegans] ref|NP_496939.2| CYtochrome P450 family member (cyp-37A1) [Caenorhabditis elegans] 1.0E-131
Protein Families
Method ID Description E-Value
p450 CPA4_MOUSE Animalia; CYP2A-4; mou; CPA4_MOUSE; Mus musculus; P15392 1.0E-100
p450 CPA4_MOUSE Animalia; CYP2A-4; mou; CPA4_MOUSE; Mus musculus; P15392 1.0E-103
p450 CPA4_MOUSE Animalia; CYP2A-4; mou; CPA4_MOUSE; Mus musculus; P15392 1.0E-110
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 6.8E-102
Pfam IPR001128 EMBL-EBI Cytochrome P450 6.8E-102
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.2E-31
Pfam IPR001128 EMBL-EBI Cytochrome P450 6.5E-64
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507109.1 2 4-26,39-57
F28G4.1 2 4-26,39-57
Sequence
Target Sequence 
>C. ELEGANS:189221_AT
ggatggactctatggtgtttggctcataacccggaatttcaagaaaaggttattcaagaa
gttgatggtatatttggaacttctgatagagactgcacaaatgatgatttgaagcaaatg
aaatatctggaaaagtgtctgaaagagtctttaagaatgtatccgtcagtgccatttttt
ggaagaacggtggagcaggatgtggttattaacggtgacttcttcccaaaaggagtccga
ataattgtgatgccactactgctccaacgcaatcctctcatcttcgataacccaaatcag
tacaacccggaaaacttcagcgaagacaaaatcggatctcgacatgcctactctgatatc
ccatttagtgctggaccccgaaattgcattggtcaaaagtttgcgatgatggaggagaaa
gcagtgatctcgtggtttttccgaaagtaccgggtcactgcgagccagcctttcgggatg
aataa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGGACTCTATGGTGTTTGGCTC 438 511 982 Antisense
GTTTGGCTCATAACCCGGAATTTCA 618 451 998 Antisense
TGTATCCGTCAGTGCCATTTTTTGG 49 561 1139 Antisense
ATAATTGTGATGCCACTACTGCTCC 683 25 1222 Antisense
TCCTCTCATCTTCGATAACCCAAAT 221 593 1254 Antisense
AAAATCGGATCTCGACATGCCTACT 384 133 1309 Antisense
TGCCTACTCTGATATCCCATTTAGT 52 581 1326 Antisense
ATTTAGTGCTGGACCCCGAAATTGC 400 15 1344 Antisense
AAAGCAGTGATCTCGTGGTTTTTCC 453 123 1399 Antisense
CCGAAAGTACCGGGTCACTGCGAGC 302 263 1422 Antisense
CGAGCCAGCCTTTCGGGATGAATAA 20 249 1442 Antisense
Sequence Source Affymetrix Proprietary Database