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[189218_at] cytochrome P450

Description R04D3.1 /REP_DB=WormBase Gene ID /WP=CE06222 /TR=Q27508 /GB=CAA94168.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 80      4grp_total > 19      6grp_cgc > 77      6grp_cgc_sub > 04_05_06      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 18.2
(-3)
A 		48.9
(1.7)
A 		38.4
(0.1)
A 		57.7
(3)
A 		40
B 40.6
(0.3)
A 		44.5
(0.7)
A 		8.1
(-3)
A 		66.5
(3)
A 		58
C 31.9
(-2.1)
A 		31.9
(-2.1)
A 		19
(-3)
A 		104.1
(3)
A 		85
avr 30.2
(-2.1) 		41.8
(-0.8) 		21.8
(-3) 		76.1
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 10.3
(-3)
A 		26.9
(-0.8)
A 		33.1
(0)
A 		55.2
(3)
A 		39.6
(0.9)
A 		10.9
(-2.9)
A 		45
B 28.4
(-2.5)
A 		45.4
(1.1)
A 		25.9
(-3)
A 		50.3
(2.1)
A 		37.2
(-0.6)
A 		54.5
(3)
A 		29
C 30.7
(-2.2)
A 		25.9
(-3)
A 		35.5
(-1.3)
A 		60.8
(3)
A 		33.1
(-1.8)
A 		60.6
(3)
A 		35
avr 23.1
(-3) 		32.7
(-1.2) 		31.5
(-1.4) 		55.4
(3) 		36.6
(-0.5) 		42
(0.5) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 6.6
(-3)
A 		10.6
(3)
A 		10
(2.1)
A 		8.4
(-0.3)
A 		4
B 13
(2.4)
A 		11
(1.4)
A 		1.9
(-3)
A 		14.2
(3)
A 		12
C 10.6
(-0.8)
A 		9.2
(-1.6)
A 		6.6
(-3)
A 		17.6
(3)
A 		11
avr 10.1
(0.2) 		10.3
(0.4) 		6.2
(-3) 		13.4
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 1.9
(-3)
A 		9.6
(0.8)
A 		7.2
(-0.4)
A 		14
(3)
A 		11.9
(2)
A 		2.7
(-2.6)
A 		12
B 9.9
(-1.5)
A 		15.1
(0.8)
A 		6.3
(-3)
A 		13.3
(0)
A 		15.1
(0.8)
A 		20.3
(3)
A 		14
C 11.4
(-1.3)
A 		7.9
(-3)
A 		9
(-2.5)
A 		12.2
(-0.9)
A 		10.7
(-1.6)
A 		20.3
(3)
A 		12
avr 7.7
(-2.8) 		10.9
(-0) 		7.5
(-3) 		13.2
(2) 		12.6
(1.4) 		14.4
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189218_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18035
Sequence Type Exemplar sequence
Representative Public ID R04D3.1 NCBI
Target Description R04D3.1 /REP_DB=WormBase Gene ID /WP=CE06222 /TR=Q27508 /GB=CAA94168.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077806(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077806 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-14A4) (cyp-14A4) mRNA, complete cds. 11/11 None
SNAP00000014106 ENSEMBL cdna:SNAP chromosome:CEL140:X:13282138:13284097:-1 9/11 None
R04D3.1 ENSEMBL cdna:known chromosome:CEL140:X:13282016:13284097:-1 gene:R04D3.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000014114 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13282015-13284097(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-14A4
Ensembl R04D3.1
Entrez Gene 187573 Entrez gene
SwissProt Q27508 EMBL-EBI
WormBase CE06222 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1582414_S_AT cytochrome P450 2D cfa
CANINE:1582413_AT cytochrome P450 2D cfa
CANINE_2:CFA.15290.1.S1_AT cytochrome P450 2D cfa
CANINE_2:CFAAFFX.2371.1.S1_S_AT cytochrome P450 2D cfa
HG-U133_PLUS_2:207498_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HG-U133A:207498_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HG-FOCUS:207498_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HG-U133A_2:207498_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HG-U95AV2:1436_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HC-G110:1436_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HG-U95AV2:38507_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HU35KSUBA:X07618_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HUGENEFL:X07618_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HUGENEFL:X16866_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HUGENEFL:X07619_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
U133_X3P:G4503222_3P_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
U133_X3P:HS.274598.1.S1_3P_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
U133_X3P:207498_3P_S_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HG-U133A_2:215809_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HG-U133_PLUS_2:215809_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
HG-U133A:215809_AT cytochrome P450, family 2, subfamily D, polypeptide 6 hs
MG-U74BV2:111163_AT cytochrome P450, family 2, subfamily d, polypeptide 22 mm
MOE430A:1419039_AT cytochrome P450, family 2, subfamily d, polypeptide 22 mm
MOUSE430A_2:1419039_AT cytochrome P450, family 2, subfamily d, polypeptide 22 mm
MOUSE430_2:1419039_AT cytochrome P450, family 2, subfamily d, polypeptide 22 mm
MOE430A:1419040_AT cytochrome P450, family 2, subfamily d, polypeptide 22 mm
MOUSE430A_2:1419040_AT cytochrome P450, family 2, subfamily d, polypeptide 22 mm
MOUSE430_2:1419040_AT cytochrome P450, family 2, subfamily d, polypeptide 22 mm
MU19KSUBB:TC33920_AT cytochrome P450, family 2, subfamily d, polypeptide 22 mm
MU19KSUBB:TC27709_AT Cytochrome P450, family 2, subfamily d, polypeptide 22, mRNA (cDNA clone MGC:28893 IMAGE:4912394) mm
RT-U34:RC_AA997886_S_AT cytochrome P450, family 2, subfamily d, polypeptide 22 rn
RG-U34A:RC_AA997886_S_AT cytochrome P450, family 2, subfamily d, polypeptide 22 rn
RT-U34:U48220_AT cytochrome P450, family 2, subfamily d, polypeptide 22 rn
RG-U34A:U48220_AT cytochrome P450, family 2, subfamily d, polypeptide 22 rn
RAE230A:1387913_AT cytochrome P450, family 2, subfamily d, polypeptide 22 rn
RAT230_2:1387913_AT cytochrome P450, family 2, subfamily d, polypeptide 22 rn
RAE230A:1370329_AT cytochrome P450, family 2, subfamily d, polypeptide 22 rn
RAT230_2:1370329_AT cytochrome P450, family 2, subfamily d, polypeptide 22 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94168 Hypothetical protein R04D3.1 [Caenorhabditis elegans] emb|CAA90619.1| Hypothetical protein R04D3.1 [Caenorhabditis elegans] ref|NP_510207.1| CYtochrome P450 family member (cyp-14A4) [Caenorhabditis elegans] 0.0
blast CAE61415 Hypothetical protein CBG05279 [Caenorhabditis briggsae] 0.0
blast CAE61409 Hypothetical protein CBG05272 [Caenorhabditis briggsae] 0.0
blast CAE61409 Hypothetical protein CBG05272 [Caenorhabditis briggsae] 1.0E-176
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-124
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-148
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.2E-119
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.3E-97
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510207.1 1 5-22
R04D3.1 1 5-22
SNAP00000014106 1 5-22
Sequence
Target Sequence 
>C. ELEGANS:189218_AT
ttcgcttcgatggcacatcgcttacatgatgaaacatcctgatattcaagacaaagttcg
taaagaaattttggatgttgttggcaactcacgttttccatcgatgtccgataaaccaaa
tatgccatatacacaagcagttattcatgaagttcagcggcattcgaatatggttccctt
cctaggaactcatcaaagcaataaggataccaacgttttaggacaaaacattccagccgg
cacaaacgtgcttgttcaagcatggtcggtcatgagaaacgaccctattttcattaatca
attgtcatttaatcccgataggtacctgatggctgatggtaaatcgttcgataagaatat
tctagagaaaaccatcccgttcagtgttggaaagagaagctgtattggagaaggactcgc
aagaatggaactctttttgattttcagtgcactaatccagaaatacgaattcattgcaaa
cggtccagttgatatgtcttatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGCTTCGATGGCACATCGCTTAC 92 697 927 Antisense
GTTGTTGGCAACTCACGTTTTCCAT 672 437 1003 Antisense
TCACGTTTTCCATCGATGTCCGATA 389 627 1015 Antisense
AGCGGCATTCGAATATGGTTCCCTT 345 81 1082 Antisense
ATGGTTCCCTTCCTAGGAACTCATC 382 51 1096 Antisense
AAAACATTCCAGCCGGCACAAACGT 142 131 1151 Antisense
AACGACCCTATTTTCATTAATCAAT 253 145 1204 Antisense
AATCCCGATAGGTACCTGATGGCTG 629 167 1237 Antisense
GAAAACCATCCCGTTCAGTGTTGGA 622 353 1293 Antisense
ACGAATTCATTGCAAACGGTCCAGT 690 89 1391 Antisense
AACGGTCCAGTTGATATGTCTTATG 15 145 1405 Antisense
Sequence Source Affymetrix Proprietary Database