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[189212_at] dehydrogenase

Description T25G12.2 /REP_DB=WormBase Gene ID /WP=CE07539 /TR=Q22787 /GB=AAC69018.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster 6grp_total > 62      4grp_total > 10      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 49
(0.1)
A 		9.9
(-3)
A 		32.2
(-1.2)
A 		84.5
(3)
A 		75
B 10.1
(-3)
A 		64.6
(3)
A 		32.4
(-0.5)
A 		56.9
(2.2)
A 		55
C 37
(-1)
A 		28.8
(-2.1)
A 		22.4
(-3)
A 		66.5
(3)
A 		44
avr 32
(-2.6) 		34.4
(-2.2) 		29
(-3) 		69.3
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 43
(0)
A 		38.1
(-0.4)
A 		78.9
(3)
A 		62.6
(1.6)
A 		22.9
(-1.7)
A 		6.9
(-3)
A 		72
B 5.9
(-3)
A 		8.4
(-2.5)
A 		35.5
(3)
A 		30.2
(1.9)
A 		13.2
(-1.5)
A 		25.4
(1)
A 		30
C 9.3
(-2.6)
A 		50
(3)
A 		13.5
(-2)
A 		6.8
(-3)
A 		40.3
(1.7)
A 		6.6
(-3)
A 		43
avr 19.4
(-1.7) 		32.2
(0.9) 		42.6
(3) 		33.2
(1.1) 		25.5
(-0.5) 		13
(-3) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 17.8
(3)
A 		2.2
(-3)
A 		8.4
(-0.6)
A 		12.4
(0.9)
A 		16
B 3.2
(-3)
A 		16
(3)
A 		7.5
(-1)
A 		12.1
(1.2)
A 		13
C 12.3
(3)
A 		8.3
(-2.3)
A 		7.8
(-3)
A 		11.2
(1.5)
A 		5
avr 11.1
(1.8) 		8.8
(-1.7) 		7.9
(-3) 		11.9
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 7.9
(-0.6)
A 		13.5
(1.6)
A 		17.2
(3)
A 		15.9
(2.5)
A 		6.9
(-1)
A 		1.7
(-3)
A 		16
B 2.1
(-3)
A 		2.8
(-2.4)
A 		8.6
(2.3)
A 		8
(1.8)
A 		5.4
(-0.3)
A 		9.5
(3)
A 		7
C 3.4
(-2.1)
A 		15.3
(3)
A 		3.4
(-2.1)
A 		1.4
(-3)
A 		13
(2)
A 		2.2
(-2.7)
A 		14
avr 4.5
(-3) 		10.5
(3) 		9.7
(2.2) 		8.4
(0.9) 		8.4
(0.9) 		4.5
(-3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189212_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17727
Sequence Type Exemplar sequence
Representative Public ID T25G12.2 NCBI
Target Description T25G12.2 /REP_DB=WormBase Gene ID /WP=CE07539 /TR=Q22787 /GB=AAC69018.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078394(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078394 NCBI Caenorhabditis elegans T25G12.2 (dehydrogenase) mRNA, complete cds. 11/11 A 
GENEFINDER00000027880 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17245883:17250348:1 11/11 None
T25G12.2 ENSEMBL cdna:known chromosome:CEL140:X:17243178:17248716:1 gene:T25G12.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027878 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17243177-17248716(+) 100.0 100.0
Public Domain and Genome References
Gene Title dehydrogenase
Ensembl T25G12.2
Entrez Gene 188907 Entrez gene
SwissProt Q22787 EMBL-EBI
WormBase CE07539 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC69018 Hypothetical protein T25G12.2 [Caenorhabditis elegans] ref|NP_510795.1| T25G12.2 [Caenorhabditis elegans] 0.0
blast AAU05548 Dehydrogenases, short chain protein 30 [Caenorhabditis elegans] ref|NP_510793.2| T25G12.7 [Caenorhabditis elegans] 1.0E-151
blast CAE69687 Hypothetical protein CBG15942 [Caenorhabditis briggsae] 1.0E-133
blast AAC69017 Hypothetical protein T25G12.1 [Caenorhabditis elegans] ref|NP_510796.1| T25G12.1 [Caenorhabditis elegans] 1.0E-119
blast AAC69018 Hypothetical protein T25G12.2 [Caenorhabditis elegans] ref|NP_510795.1| T25G12.2 [Caenorhabditis elegans] 2.0E-87
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 2.7E-38
Sequence
Target Sequence 
>C. ELEGANS:189212_AT
ttttctttcctcctaattgactcggctgacatgcatctcaaagggaaacttttttctcac
caccagcactatcaccatgatcatcatcaagaaattgaagcatcgatacctctctcactc
tctcgccgtacaagaaagacggcgcgaagacatctcgtgtcaaaagaacacttcctcaac
aaggtgaagattgaggctgcactggaacgtttcaacagagatgaagtcgtgtattatcaa
ggaggacagagagtactatgtgaactaacagtcacagaaaacagaccgtgtcgactcctg
atcgggcggaacaagcggtcacttctcaactacatgcaattggagttagaaaccggcatt
tccgacgaacaattggtgctcactgacatggcgggtgccgaatttccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTCTTTCCTCCTAATTGACTCGG 259 675 1153 Antisense
AATTGACTCGGCTGACATGCATCTC 409 179 1167 Antisense
AATTGAAGCATCGATACCTCTCTCA 306 179 1245 Antisense
TCACTCTCTCGCCGTACAAGAAAGA 350 627 1267 Antisense
AAGACGGCGCGAAGACATCTCGTGT 695 153 1288 Antisense
GATTGAGGCTGCACTGGAACGTTTC 557 425 1341 Antisense
AAAACAGACCGTGTCGACTCCTGAT 250 129 1430 Antisense
GACTCCTGATCGGGCGGAACAAGCG 373 375 1445 Antisense
GGTCACTTCTCAACTACATGCAATT 642 503 1469 Antisense
TCCGACGAACAATTGGTGCTCACTG 660 591 1513 Antisense
GACATGGCGGGTGCCGAATTTCCGA 570 363 1537 Antisense
Sequence Source Affymetrix Proprietary Database