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[189211_s_at] RNA helicase

Description ZK512.2 /REP_DB=WormBase Gene ID /WP=CE03821 /TR=SW:P34640 /GB=CAA80151.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=RNA helicase
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 30      4grp_high_8_8 > 04      6grp_highest_nonorm > 84      6grp_total > 77      4grp_total > 04      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 853
1 2 3 4 range
A 857.3
(-0.8)
P 		593.4
(-3)
P 		699.5
(-2.1)
P 		1299.9
(3)
P 		707
B 831.1
(-1.5)
P 		623.5
(-3)
P 		744.8
(-2.1)
P 		1446.1
(3)
P 		823
C 870.2
(-1.1)
P 		658.4
(-3)
P 		768.6
(-2)
P 		1310.4
(3)
P 		652
avr 852.9
(-1.1) 		625.1
(-3) 		737.6
(-2.1) 		1352.1
(3) 		727

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 505
1 2 3 4 5 6 range
A 429.1
(-3)
P 		544.6
(-1)
P 		471.6
(-2.3)
P 		534.6
(-1.2)
P 		564.8
(-0.6)
P 		771.4
(3)
P 		342
B 517.9
(-2.1)
P 		505.2
(-2.3)
P 		444.1
(-3)
P 		547.5
(-1.7)
P 		678.5
(-0.1)
P 		934
(3)
P 		490
C 532.8
(-2.1)
P 		534.4
(-2.1)
P 		479.1
(-3)
P 		568.8
(-1.5)
P 		531.7
(-2.1)
P 		843.3
(3)
P 		364
avr 493.3
(-2.6) 		528.1
(-2) 		464.9
(-3) 		550.3
(-1.7) 		591.7
(-1) 		849.6
(3) 		385
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 312.1
(3)
P 		128.8
(-3)
P 		182.9
(-1.2)
P 		190.3
(-1)
P 		183
B 265
(1.3)
P 		154
(-3)
P 		172.6
(-2.3)
P 		308.2
(3)
P 		154
C 288.1
(3)
P 		189.3
(-3)
P 		267.9
(1.8)
P 		221
(-1.1)
P 		99
avr 288.4
(3) 		157.4
(-3) 		207.8
(-0.7) 		239.8
(0.8) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 269
1 2 3 4 5 6 range
A 79.1
(-3)
P 		193.7
(3)
P 		102.7
(-1.8)
P 		135.6
(-0)
P 		170.4
(1.8)
P 		187.8
(2.7)
P 		115
B 180.2
(-1.2)
P 		168.2
(-1.5)
P 		107.8
(-3)
P 		145
(-2.1)
P 		275.7
(1.2)
P 		348.3
(3)
P 		241
C 197.5
(-0)
P 		163.1
(-1.3)
P 		121.9
(-2.7)
P 		114.4
(-3)
P 		172.1
(-0.9)
P 		282.3
(3)
P 		168
avr 152.3
(-1.5) 		175
(-0.6) 		110.8
(-3) 		131.7
(-2.2) 		206.1
(0.5) 		272.8
(3) 		162
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189211_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7802
Sequence Type Exemplar sequence
Representative Public ID ZK512.2 NCBI
Target Description ZK512.2 /REP_DB=WormBase Gene ID /WP=CE03821 /TR=SW:P34640 /GB=CAA80151.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=RNA helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066624(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066624 NCBI Caenorhabditis elegans ZK512.2 (ZK512.2) mRNA, complete cds. 11/11 None
SNAP00000002534 ENSEMBL cdna:SNAP chromosome:CEL140:III:9141817:9144692:-1 11/11 None
GENEFINDER00000002544 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9141817:9144692:-1 11/11 None
ZK512.2.1 ENSEMBL cdna:known chromosome:CEL140:III:9141725:9144700:-1 gene:ZK512.2 11/11 None
ZK512.2.2 ENSEMBL cdna:known chromosome:CEL140:III:9141725:9144793:-1 gene:ZK512.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9141812-9144688(-) 100.0 100.0
Public Domain and Genome References
Gene Title RNA helicase
Ensembl ZK512.2
Entrez Gene 176292 Entrez gene
SwissProt P34640 EMBL-EBI
WormBase CE03821 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA80151 Hypothetical protein ZK512.2 [Caenorhabditis elegans] ref|NP_499025.1| ZK512.2 [Caenorhabditis elegans] sp|P34640|YOQ2_CAEEL Putative ATP-dependent RNA helicase ZK512.2 in chromosome III 0.0
blast CAE62660 Hypothetical protein CBG06798 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 8.4E-24
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.5E-65
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 8.4E-24
Sequence
Target Sequence 
>C. ELEGANS:189211_S_AT
tcaagtttctgatcgtgcaattctagaagctggaactcgtgcatttgtgagtcacgtgga
gtcatatgccaaacacgattgtcatctcatttgctcacttgatgatctcaacgtggtcgg
tcttgccaattcgtacgctcttctcagattgccgaaaatgcgtgaactgtcacaaagaaa
agatttggatatgttcgacagaagtgccatcgagacatctgaaatcaaatacgccgatgt
taaactagaagccaatcgggaaactgtgatgaaagaaaagcacgagaagaaggtcgagac
gttggctgcaaaggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAAGTTTCTGATCGTGCAATTCTA 52 633 1227 Antisense
GAAGCTGGAACTCGTGCATTTGTGA 407 341 1252 Antisense
GTGAGTCACGTGGAGTCATATGCCA 57 485 1273 Antisense
ATATGCCAAACACGATTGTCATCTC 498 21 1290 Antisense
TTGTCATCTCATTTGCTCACTTGAT 293 707 1305 Antisense
TGATGATCTCAACGTGGTCGGTCTT 91 567 1326 Antisense
CAATTCGTACGCTCTTCTCAGATTG 568 179 1353 Antisense
GATTGCCGAAAATGCGTGAACTGTC 310 433 1373 Antisense
GGATATGTTCGACAGAAGTGCCATC 497 509 1413 Antisense
GAAGTGCCATCGAGACATCTGAAAT 106 337 1427 Antisense
GGTCGAGACGTTGGCTGCAAAGGAT 630 501 1518 Antisense
Sequence Source Affymetrix Proprietary Database