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[189210_at] protein kinase

Description F46F5.2 /REP_DB=WormBase Gene ID /WP=CE20822 /TR=Q9TXX4 /GB=AAC78196.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 70      4grp_total > 26      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 1.3
(-3)
A 		39.5
(3)
A 		1.1
(-3)
A 		32.8
(2)
A 		38
B 3.9
(-3)
A 		5.2
(-2.2)
A 		13.8
(3)
A 		5
(-2.3)
A 		10
C 1.2
(-2.9)
A 		16.1
(0)
A 		0.9
(-3)
A 		31.3
(3)
A 		30
avr 2.1
(-3) 		20.3
(2.2) 		5.3
(-2.1) 		23
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 2.2
(-2.8)
A 		1.7
(-3)
A 		1.8
(-3)
A 		14.8
(3)
A 		1.9
(-2.9)
A 		5.9
(-1.1)
A 		13
B 8.5
(-1.5)
A 		0.6
(-3)
A 		33.2
(3)
A 		1.6
(-2.8)
A 		9.5
(-1.4)
A 		1
(-2.9)
A 		33
C 7.8
(-2)
A 		1.9
(-2.9)
A 		1.5
(-3)
A 		39.7
(3)
A 		1.6
(-3)
A 		16.1
(-0.7)
A 		38
avr 6.2
(-1.3) 		1.4
(-3) 		12.2
(0.7) 		18.7
(3) 		4.3
(-2) 		7.7
(-0.8) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 0.5
(-2.9)
A 		8.6
(3)
A 		0.3
(-3)
A 		4.8
(0.3)
A 		8
B 1.2
(-2.7)
A 		1.3
(-2.4)
A 		3.2
(3)
A 		1.1
(-3)
A 		2
C 0.4
(-2.9)
A 		4.6
(2.2)
A 		0.3
(-3)
A 		5.3
(3)
A 		5
avr 0.7
(-3) 		4.8
(3) 		1.3
(-2.1) 		3.7
(1.4) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.4
(-3)
A 		0.6
(-2.6)
A 		0.4
(-3)
A 		3.7
(3)
A 		0.6
(-2.6)
A 		1.4
(-1.2)
A 		3
B 2.9
(-0.9)
A 		0.2
(-3)
A 		8.1
(3)
A 		0.4
(-2.8)
A 		3.9
(-0.2)
A 		0.4
(-2.8)
A 		8
C 2.9
(-1)
A 		0.6
(-2.8)
A 		0.4
(-3)
A 		8
(3)
A 		0.5
(-2.9)
A 		5.4
(0.9)
A 		8
avr 2.1
(-0.3) 		0.5
(-3) 		3
(1.3) 		4
(3) 		1.7
(-0.9) 		2.4
(0.3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189210_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5267
Sequence Type Exemplar sequence
Representative Public ID F46F5.2 NCBI
Target Description F46F5.2 /REP_DB=WormBase Gene ID /WP=CE20822 /TR=Q9TXX4 /GB=AAC78196.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061411(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061411 NCBI Caenorhabditis elegans F46F5.2 (F46F5.2) mRNA, complete cds. 11/11 None
SNAP00000029514 ENSEMBL cdna:SNAP chromosome:CEL140:II:796280:797824:-1 9/11 None
GENEFINDER00000029535 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:789978:798264:-1 11/11 None
F46F5.2 ENSEMBL cdna:known chromosome:CEL140:II:796590:797824:-1 gene:F46F5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:796589-797824(-) 100.0 100.0
chrII:9409582-9410823(-) 90.66 90.66
Public Domain and Genome References
Gene Title protein kinase
Ensembl F46F5.2
Entrez Gene 185856 Entrez gene
SwissProt Q9TXX4 EMBL-EBI
WormBase CE20822 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC78196 Hypothetical protein F46F5.2 [Caenorhabditis elegans] ref|NP_493812.1| F46F5.2 [Caenorhabditis elegans] 0.0
blast T23792 hypothetical protein M176.9 - Caenorhabditis elegans 0.0
blast AAC78183 Hypothetical protein F46F5.1 [Caenorhabditis elegans] ref|NP_493811.1| F46F5.1 [Caenorhabditis elegans] 0.0
blast NP_501307 T06C10.3 [Caenorhabditis elegans] gb|AAA82300.1| Hypothetical protein T06C10.3 [Caenorhabditis elegans] 1.0E-146
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 7.2E-8
Pfam IPR000719 EMBL-EBI Protein kinase 5.7E-51
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-43
Pfam IPR000980 EMBL-EBI SH2 motif 7.2E-8
Pfam IPR000719 EMBL-EBI Protein kinase 5.7E-51
Sequence
Target Sequence 
>C. ELEGANS:189210_AT
gttcatcagaaacttttctcataaaaacgttgtacgcctgtatggagttgctcatgacga
gcagccactgtacattctgcttgagttggtcaaaggtggatccttgcaagattacatgaa
gaagacgaaatcggttcctttggcggatagaatcaatttttgtcttggagctgcaaaagg
tcttgcgtacttgcatcaaaactattgtatccaccgggacatcgctgcgagaaattgtct
tcttcacgagaacgaagtcaagatcaccgatttcggattatctcgtgctggaccatctta
tacaacaaagacatcttgtaagcttccggtaaagtggctcgcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCATCAGAAACTTTTCTCATAAA 93 445 693 Antisense
ACGTTGTACGCCTGTATGGAGTTGC 386 93 719 Antisense
GAAGACGAAATCGGTTCCTTTGGCG 314 339 813 Antisense
ATCGGTTCCTTTGGCGGATAGAATC 474 35 822 Antisense
TCAAAACTATTGTATCCACCGGGAC 496 629 888 Antisense
CCACCGGGACATCGCTGCGAGAAAT 46 275 903 Antisense
CATCGCTGCGAGAAATTGTCTTCTT 472 211 912 Antisense
CACCGATTTCGGATTATCTCGTGCT 32 201 957 Antisense
ATTATCTCGTGCTGGACCATCTTAT 639 13 969 Antisense
AAGACATCTTGTAAGCTTCCGGTAA 23 153 1000 Antisense
TAAGCTTCCGGTAAAGTGGCTCGCG 184 637 1011 Antisense
Sequence Source Affymetrix Proprietary Database