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[189209_at] ser/thr kinase

Description B0218.5 /REP_DB=WormBase Gene ID /WP=CE06688 /TR=Q17448 /GB=AAB00666.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ser-thr kinase
Cluster 6grp_total > 77      4grp_total > 45      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 17.6
(3)
A 		9.7
(-0.4)
A 		3.7
(-2.9)
A 		3.5
(-3)
A 		14
B 9.7
(3)
A 		7.5
(1.3)
A 		3.2
(-2)
A 		1.9
(-3)
A 		8
C 10.1
(0.2)
A 		15.2
(3)
A 		4.1
(-3)
A 		10.6
(0.5)
A 		11
avr 12.5
(3) 		10.8
(1.8) 		3.7
(-3) 		5.3
(-1.9) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 2.5
(-3)
A 		28.8
(3)
A 		3
(-2.9)
A 		10.6
(-1.2)
A 		14.6
(-0.2)
A 		8.9
(-1.5)
A 		26
B 3.9
(-2.9)
A 		4
(-2.9)
A 		3.4
(-3)
A 		8.8
(-2.4)
A 		5.9
(-2.7)
A 		55.1
(3)
A 		52
C 2.6
(-3)
A 		3.1
(-2.9)
A 		3.8
(-2.9)
A 		6.6
(-2.6)
A 		25.5
(-0.4)
A 		56.4
(3)
A 		54
avr 3
(-3) 		12
(-1.5) 		3.4
(-2.9) 		8.7
(-2.1) 		15.3
(-1) 		40.1
(3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 6.4
(3)
A 		2.1
(-1.4)
A 		1
(-2.5)
A 		0.5
(-3)
A 		6
B 3.1
(3)
A 		1.8
(0.1)
A 		0.7
(-2.3)
A 		0.4
(-3)
A 		3
C 3.4
(1)
A 		4.4
(3)
A 		1.4
(-3)
A 		1.8
(-2.2)
A 		3
avr 4.3
(3) 		2.8
(0.4) 		1
(-2.8) 		0.9
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 0.5
(-3)
A 		10.2
(3)
A 		0.7
(-2.9)
A 		2.7
(-1.6)
A 		4.4
(-0.6)
A 		2.2
(-1.9)
A 		10
B 1.4
(-2.8)
A 		1.3
(-2.8)
A 		0.8
(-3)
A 		2.3
(-2.5)
A 		2.4
(-2.5)
A 		20.5
(3)
A 		20
C 0.9
(-3)
A 		0.9
(-3)
A 		1
(-3)
A 		1.3
(-2.9)
A 		8.2
(-0.6)
A 		18.9
(3)
A 		18
avr 0.9
(-3) 		4.1
(-1.5) 		0.8
(-3) 		2.1
(-2.4) 		5
(-1.1) 		13.9
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189209_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9394
Sequence Type Exemplar sequence
Representative Public ID B0218.5 NCBI
Target Description B0218.5 /REP_DB=WormBase Gene ID /WP=CE06688 /TR=Q17448 /GB=AAB00666.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ser-thr kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068966(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068966 NCBI Caenorhabditis elegans B0218.5 (B0218.5) mRNA, complete cds. 11/11 None
SNAP00000005554 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8155235:8156428:-1 11/11 None
GENEFINDER00000005559 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8155235:8156428:-1 11/11 None
B0218.5 ENSEMBL cdna:known chromosome:CEL140:IV:8155235:8156428:-1 gene:B0218.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8155240-8156434(-) 100.0 100.0
Public Domain and Genome References
Gene Title ser/thr kinase
Ensembl B0218.5
Entrez Gene 181853 Entrez gene
SwissProt Q17448 EMBL-EBI
WormBase CE06688 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00666 Hypothetical protein B0218.5 [Caenorhabditis elegans] ref|NP_501367.1| B0218.5 [Caenorhabditis elegans] 0.0
blast CAE71216 Hypothetical protein CBG18079 [Caenorhabditis briggsae] 1.0E-166
blast AAC26933 Hypothetical protein F36H12.9 [Caenorhabditis elegans] ref|NP_500758.1| F36H12.9 [Caenorhabditis elegans] 1.0E-149
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-5
Pfam IPR000719 EMBL-EBI Protein kinase 9.1E-5
Sequence
Target Sequence 
>C. ELEGANS:189209_AT
atcacacaatcttcatgctcgacttcggtctctgccgtgaatttgtgaaacgaggcgaag
gcaggcttcggactcaacgtgcaaagtctcaattccgaggaacaactcgttatgctccaa
tcaattcaatgcttgaaattgataccggacgcaaagatgacattgaatcatggctttaca
tggtcgcggaatggacatctggtggattgccatggagaaagttcaaggccacagaacggg
aaaaagttctgaagtacaagaaagacgtgagaacggacaaggaaatcatggcggatttgt
tttataattgcccgttgaaggagtttgaacgaattttgaagtacgtagacgaacttgact
tctactcggagcccgattataagttcgtgtactgctgtcttcaacacgctgccgctgcca
gcaagatcaaggatacagatccattggattgggatccaaatgttccgtacatgggcccga
ttgaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCACACAATCTTCATGCTCGACTT 454 27 500 Antisense
ACTTCGGTCTCTGCCGTGAATTTGT 398 103 521 Antisense
GGCGAAGGCAGGCTTCGGACTCAAC 526 539 553 Antisense
CAACGTGCAAAGTCTCAATTCCGAG 447 187 574 Antisense
GAACAACTCGTTATGCTCCAATCAA 260 353 599 Antisense
ATGGCTTTACATGGTCGCGGAATGG 313 51 669 Antisense
GGACATCTGGTGGATTGCCATGGAG 625 523 692 Antisense
GACGAACTTGACTTCTACTCGGAGC 56 379 847 Antisense
TAAGTTCGTGTACTGCTGTCTTCAA 33 637 879 Antisense
GATTGGGATCCAAATGTTCCGTACA 217 435 946 Antisense
TTCCGTACATGGGCCCGATTGAAAC 82 699 962 Antisense
Sequence Source Affymetrix Proprietary Database