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[189202_at]

Description R151.4 /REP_DB=WormBase Gene ID /WP=CE00747 /TR=Q21996 /GB=AAK29851.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Tyrosine kinase
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 23      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 70      6grp_total > 22      4grp_total > 21      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 268.8
(3)
P 		243.7
(1.1)
P 		187.6
(-3)
P 		251.8
(1.7)
P 		81
B 309.9
(3)
P 		191.7
(-1.5)
P 		150.7
(-3)
P 		250.7
(0.8)
P 		159
C 217.1
(-1.1)
P 		197.9
(-1.6)
P 		144.1
(-3)
P 		373.7
(3)
P 		230
avr 265.3
(1.8) 		211.1
(-0.7) 		160.8
(-3) 		292.1
(3) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 344.2
(2.1)
P 		374.2
(3)
P 		337.3
(1.9)
P 		328.9
(1.6)
P 		173.8
(-3)
P 		190.5
(-2.5)
P 		200
B 316.3
(2.7)
P 		324.3
(3)
P 		253.6
(0.3)
P 		257.7
(0.5)
P 		210.1
(-1.3)
P 		166.6
(-3)
P 		158
C 321
(1)
P 		372.1
(2.2)
P 		295.1
(0.4)
P 		406.5
(3)
P 		183.4
(-2.2)
P 		147.6
(-3)
P 		259
avr 327.2
(2.1) 		356.9
(3) 		295.3
(1) 		331
(2.2) 		189.1
(-2.3) 		168.2
(-3) 		189
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 97.9
(3)
P 		52.9
(-1.4)
P 		49.1
(-1.8)
P 		36.9
(-3)
P 		61
B 98.8
(3)
P 		47.3
(-1.8)
P 		34.9
(-3)
P 		53.4
(-1.3)
P 		64
C 71.9
(3)
P 		56.9
(-1.1)
P 		50.2
(-3)
P 		63
(0.5)
P 		22
avr 89.5
(3) 		52.4
(-2) 		44.7
(-3) 		51.1
(-2.1) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 63.4
(-1.8)
P 		133.1
(3)
P 		73.4
(-1.1)
P 		83.4
(-0.4)
P 		52.4
(-2.6)
P 		46.4
(-3)
P 		87
B 110.1
(3)
P 		108
(2.7)
P 		61.6
(-3)
P 		68.2
(-2.2)
P 		85.4
(-0.1)
P 		62.1
(-2.9)
P 		49
C 119
(3)
P 		113.5
(2.5)
P 		75.1
(-0.8)
P 		81.7
(-0.2)
P 		59.4
(-2.1)
P 		49.4
(-3)
P 		70
avr 97.5
(1.1) 		118.2
(3) 		70
(-1.4) 		77.8
(-0.7) 		65.7
(-1.8) 		52.6
(-3) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189202_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6599
Sequence Type Exemplar sequence
Representative Public ID R151.4 NCBI
Target Description R151.4 /REP_DB=WormBase Gene ID /WP=CE00747 /TR=Q21996 /GB=AAK29851.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Tyrosine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027558(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027558 NCBI Caenorhabditis elegans R151.4a (R151.4) mRNA, complete cds. 11/11 None
GENEFINDER00000037946 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7218737:7220990:1 11/11 None
SNAP00000037936 ENSEMBL cdna:SNAP chromosome:CEL140:III:7219898:7220990:1 11/11 None
R151.4a ENSEMBL cdna:known chromosome:CEL140:III:7219898:7221110:1 gene:R151.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027559 3/11 Cross Hyb Matching Probes None
R151.4b 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176017 2 1 NM_001027558 189202_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7219895-7220988(+) 100.0 100.0
Public Domain and Genome References
Ensembl R151.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAP82638 Hypothetical protein R151.4a [Caenorhabditis elegans] ref|NP_001022729.1| R151.4a [Caenorhabditis elegans] pir||T16765 hypothetical protein R151.4 - Caenorhabditis elegans 1.0E-148
blast AAP82638 Hypothetical protein R151.4a [Caenorhabditis elegans] ref|NP_001022729.1| R151.4a [Caenorhabditis elegans] pir||T16765 hypothetical protein R151.4 - Caenorhabditis elegans 1.0E-146
blast CAE70158 Hypothetical protein CBG16625 [Caenorhabditis briggsae] 2.0E-96
blast CAE70158 Hypothetical protein CBG16625 [Caenorhabditis briggsae] 7.0E-96
blast AAK29852 Hypothetical protein R151.1 [Caenorhabditis elegans] ref|NP_498588.1| R151.1 [Caenorhabditis elegans] 2.0E-90
blast EAL39285 ENSANGP00000028911 [Anopheles gambiae str. PEST] ref|XP_554059.1| ENSANGP00000028911 [Anopheles gambiae str. PEST] 5.0E-61
Protein Families
Method ID Description E-Value
hanks 1.2.17 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; PKCi 3.0E-61
hanks 1.2.10 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; AplII 3.0E-61
hanks 1.2.10 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; AplII 3.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-85
Pfam IPR000719 EMBL-EBI Protein kinase 6.0E-82
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000037946 1 113-135
Sequence
Target Sequence 
>C. ELEGANS:189202_AT
ggactcagacagtgcgattgattccaaagaattcttgtcatttacgaatcaaattgcttg
tggaatggctcatcttgaatcagttgggattattcacagagatcttgctgcaagaaatat
tctcgttggaacgggaaaagttctgaaaatctccgattttggaatgtctcgtcctggagt
ttatatcaaaatgagcaaaggagtgattccacttcgttggctgtctcctgaagcaattaa
agataatacttattcaaataagagcgatgtttgggcttttggagttttactctgggaaat
agccactcttggaggattcccgtataacaatgtggcagataaggatattctaaatcaatt
gactgaaggaatgagactggaacaacctgccaaatgttctgatgatatgtacattttaat
gaaatcatgttggaatttgaaagcagaagaccgtccatctttcttggcaattttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACTCAGACAGTGCGATTGATTCC 490 525 297 Antisense
GAATGGCTCATCTTGAATCAGTTGG 564 331 359 Antisense
TGGAATGTCTCGTCCTGGAGTTTAT 365 545 456 Antisense
AAGGAGTGATTCCACTTCGTTGGCT 321 161 494 Antisense
TTCGTTGGCTGTCTCCTGAAGCAAT 290 693 509 Antisense
GCGATGTTTGGGCTTTTGGAGTTTT 265 291 560 Antisense
AATAGCCACTCTTGGAGGATTCCCG 111 171 594 Antisense
GAGGATTCCCGTATAACAATGTGGC 547 403 608 Antisense
ACAACCTGCCAAATGTTCTGATGAT 477 113 678 Antisense
AAGCAGAAGACCGTCCATCTTTCTT 74 151 737 Antisense
CCGTCCATCTTTCTTGGCAATTTTG 472 265 747 Antisense
Sequence Source Affymetrix Proprietary Database