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[189200_at] YK10 homolog

Description T07A5.2 /REP_DB=WormBase Gene ID /WP=CE01647 /TR=SW:Q10045 /GB=CAA88132.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=YK10 homolog
Cluster 6grp_total > 13      4grp_total > 50      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 74.6
(0.2)
A 		126.2
(3)
A 		110.9
(2.2)
A 		15.2
(-3)
A 		111
B 70.4
(-3)
A 		94.8
(0.8)
A 		97.9
(1.3)
M 		109.1
(3)
A 		39
C 100.4
(3)
A 		83.2
(-3)
A 		84.6
(-2.5)
M 		95.6
(1.3)
A 		17
avr 81.8
(-1.2) 		101.4
(3) 		97.8
(2.2) 		73.3
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 112.4
(3)
A 		56.9
(-1.8)
A 		55.9
(-1.9)
A 		63.5
(-1.2)
A 		42.7
(-3)
A 		49.9
(-2.4)
A 		70
B 124.8
(3)
P 		106.1
(1.6)
M 		81.8
(-0.2)
A 		105.2
(1.5)
M 		45.1
(-3)
A 		48.3
(-2.8)
A 		80
C 90.7
(1.5)
P 		86.2
(1.2)
P 		86.3
(1.2)
A 		110.6
(3)
A 		59
(-0.9)
A 		30.2
(-3)
A 		80
avr 109.3
(3) 		83.1
(0.6) 		74.7
(-0.1) 		93.1
(1.5) 		48.9
(-2.4) 		42.8
(-3) 		67
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 27.2
(2.6)
A 		27.4
(2.6)
A 		29
(3)
A 		2.2
(-3)
A 		27
B 22.5
(-3)
A 		23.4
(3)
A 		22.7
(-1.7)
M 		23.2
(1.7)
A 		1
C 33.2
(3)
A 		23.9
(-0.3)
A 		29.5
(1.7)
M 		16.1
(-3)
A 		17
avr 27.6
(3) 		24.9
(1.8) 		27.1
(2.8) 		13.8
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 20.7
(3)
A 		20.2
(2.7)
A 		12.2
(-2.9)
A 		16.1
(-0.2)
A 		12.9
(-2.4)
A 		12.1
(-3)
A 		9
B 43.4
(3)
P 		35.3
(1.1)
M 		19.9
(-2.6)
A 		27.9
(-0.7)
M 		18.3
(-2.9)
A 		18
(-3)
A 		25
C 33.6
(3)
P 		26.3
(1.1)
P 		22
(0)
A 		22.2
(0.1)
A 		19.1
(-0.7)
A 		10.1
(-3)
A 		24
avr 32.6
(3) 		27.3
(1.3) 		18
(-1.6) 		22.1
(-0.3) 		16.8
(-1.9) 		13.4
(-3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189200_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6460
Sequence Type Exemplar sequence
Representative Public ID T07A5.2 NCBI
Target Description T07A5.2 /REP_DB=WormBase Gene ID /WP=CE01647 /TR=SW:Q10045 /GB=CAA88132.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=YK10 homolog
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066878(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066878 NCBI Caenorhabditis elegans T07A5.2 (T07A5.2) mRNA, complete cds. 11/11 None
SNAP00000030112 ENSEMBL cdna:SNAP chromosome:CEL140:III:10314775:10316250:-1 11/11 None
GENEFINDER00000030118 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10314775:10318445:-1 11/11 None
T07A5.2 ENSEMBL cdna:known chromosome:CEL140:III:10314472:10318039:-1 gene:T07A5.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10314770-10316246(-) 100.0 100.0
Public Domain and Genome References
Gene Title YK10 homolog
Ensembl T07A5.2
Entrez Gene 176443 Entrez gene
SwissProt Q10045 EMBL-EBI
WormBase CE36501 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13583_AT UNC-50 family protein at
ATH1-121501:263303_AT UNC-50 family protein at
CANINE:1591503_AT similar to unc-50 homolog cfa
CANINE_2:CFA.10690.1.S1_AT similar to unc-50 homolog cfa
CANINE_2:CFA.10690.1.S1_S_AT similar to unc-50 homolog cfa
DROSOPHILA_2:1635637_AT dm
DROSGENOME1:149516_AT dm
CHICKEN:GGA.12244.1.S1_AT unc-50 homolog (C. elegans) gga
HG-U133A_2:203583_AT unc-50 homolog (C. elegans) hs
HG-U133A:203583_AT unc-50 homolog (C. elegans) hs
HG-U133_PLUS_2:203583_AT unc-50 homolog (C. elegans) hs
U133_X3P:G7661609_3P_AT unc-50 homolog (C. elegans) hs
HG-U95AV2:39442_AT unc-50 homolog (C. elegans) hs
HG-U133_PLUS_2:239505_AT Unc-50 homolog (C. elegans) hs
HG-U133B:239505_AT Unc-50 homolog (C. elegans) hs
HG-U95D:76392_AT Unc-50 homolog (C. elegans) hs
U133_X3P:HS.121740.0.S1_3P_AT Unc-50 homolog (C. elegans) hs
MOUSE430_2:1421982_A_AT unc-50 homolog (C. elegans) mm
MOUSE430A_2:1421982_A_AT unc-50 homolog (C. elegans) mm
MOE430A:1421982_A_AT unc-50 homolog (C. elegans) mm
MG-U74AV2:96675_AT unc-50 homolog (C. elegans) mm
MOE430A:1435812_AT unc-50 homolog (C. elegans) mm
MOUSE430A_2:1435812_AT unc-50 homolog (C. elegans) mm
MOUSE430_2:1435812_AT unc-50 homolog (C. elegans) mm
MOE430A:1435811_A_AT unc-50 homolog (C. elegans) mm
MOUSE430A_2:1435811_A_AT unc-50 homolog (C. elegans) mm
MOUSE430_2:1435811_A_AT unc-50 homolog (C. elegans) mm
MU19KSUBB:TC27432_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
MU19KSUBB:TC27432_G_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
MU19KSUBB:TC27433_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
MU19KSUBC:TC38061_S_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
MU19KSUBC:TC38062_AT Unc-50 homolog (C. elegans), mRNA (cDNA clone MGC:28445 IMAGE:4039903) mm
RG-U34A:RC_AI008423_AT unc-50 homolog (C. elegans) rn
RAE230A:1398865_AT unc-50 homolog (C. elegans) rn
RAT230_2:1398865_AT unc-50 homolog (C. elegans) rn
YEAST_2:1776281_AT Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Sc
Protein Similarities
Method ID Description E-Value
blast CAE56800 Hypothetical protein CBG24612 [Caenorhabditis briggsae] 1.0E-168
blast CAA88132 Hypothetical protein T07A5.2 [Caenorhabditis elegans] ref|NP_499279.2| T07A5.2 [Caenorhabditis elegans] sp|Q10045|YRT2_CAEEL Hypothetical protein T07A5.2 in chromosome III 1.0E-165
blast CAE56800 Hypothetical protein CBG24612 [Caenorhabditis briggsae] 1.0E-153
blast CAA88132 Hypothetical protein T07A5.2 [Caenorhabditis elegans] ref|NP_499279.2| T07A5.2 [Caenorhabditis elegans] sp|Q10045|YRT2_CAEEL Hypothetical protein T07A5.2 in chromosome III 1.0E-131
blast CAE56800 Hypothetical protein CBG24612 [Caenorhabditis briggsae] 1.0E-125
blast XP_785090 PREDICTED: similar to unc-50 homolog [Strongylocentrotus purpuratus] 8.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR007881 EMBL-EBI UNC-50 1.0E-126
Pfam IPR007881 EMBL-EBI UNC-50 1.0E-126
Pfam IPR007881 EMBL-EBI UNC-50 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499279.2 5 116-138,148-170,191-213,228-250,255-277
T07A5.2 5 116-138,148-170,191-213,228-250,255-277
SNAP00000030112 5 59-81,91-113,134-156,171-193,198-220
GENEFINDER00000030118 6 5-27,175-197,207-229,250-272,287-309,314-336
Sequence
Target Sequence 
>C. ELEGANS:189200_AT
tcttttcttcaatattctacgcctacgctctgggccttgagaaaatcggatttttcacgt
ttttcctctggtctgtctttgttgactgtatcggagtgggcgtggtcatcgctacggtgc
tctggtgggtctcgaaccgatttctacggaaagttcgtgaccaggacgtcgagtggggtt
attgcttcgatgtacatctaaacgcgttcttcccgatgctcattttgctgcatgtcatcg
tgccgattttgtatccaactctcatcgactcgccggcattcctctccatcctgcttggca
acacgttctggttcctggccgcgtgctactacgtctacatcacatttctcggctacacgg
ctcttccgattctccacaaaactcaatacttcctctatcccatctcgttcattttcatgt
tctttgtggcgacactgaccggcggatggaacatttcacggactgctctcaacttttatc
attc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTTTCTTCAATATTCTACGCCTA 679 615 218 Antisense
TCGGATTTTTCACGTTTTTCCTCTG 270 605 263 Antisense
TTTCCTCTGGTCTGTCTTTGTTGAC 680 669 279 Antisense
GTGGGTCTCGAACCGATTTCTACGG 236 487 342 Antisense
ATGTACATCTAAACGCGTTCTTCCC 196 49 407 Antisense
TTTTGCTGCATGTCATCGTGCCGAT 670 673 440 Antisense
GATTTTGTATCCAACTCTCATCGAC 640 429 462 Antisense
TACGTCTACATCACATTTCTCGGCT 178 649 547 Antisense
ACAAAACTCAATACTTCCTCTATCC 145 113 593 Antisense
TATCCCATCTCGTTCATTTTCATGT 327 659 613 Antisense
GGACTGCTCTCAACTTTTATCATTC 341 523 677 Antisense
Sequence Source Affymetrix Proprietary Database