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[189199_s_at]

Description ZK354.2 /REP_DB=WormBase Gene ID /WP=CE15314 /TR=P91571 /GB=AAB42257.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 89      4grp_total > 43      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 2.3
(-2.7)
A 		4.5
(3)
A 		2.2
(-3)
A 		3.3
(-0.1)
A 		2
B 3.2
(-2.2)
A 		9.4
(3)
A 		4.8
(-0.9)
A 		2.3
(-3)
A 		7
C 2.6
(-2.6)
A 		6.5
(3)
A 		2.3
(-3)
A 		5.3
(1.3)
A 		4
avr 2.7
(-3) 		6.8
(3) 		3.1
(-2.4) 		3.6
(-1.7) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 2.6
(-2.8)
A 		10.1
(-0.6)
A 		1.9
(-3)
A 		4.1
(-2.4)
A 		6.2
(-1.8)
A 		22.7
(3)
A 		21
B 4.7
(-2.8)
A 		8.1
(-2.4)
A 		4.1
(-2.9)
A 		3.3
(-3)
A 		48.8
(2.8)
A 		50
(3)
A 		47
C 2.7
(-2.9)
A 		2.6
(-2.9)
A 		1.8
(-3)
A 		3.2
(-2.9)
A 		61.1
(1.1)
A 		89.1
(3)
A 		87
avr 3.3
(-2.9) 		6.9
(-2.5) 		2.6
(-3) 		3.5
(-2.9) 		38.7
(1.2) 		53.9
(3) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 0.8
(0.6)
A 		1
(3)
A 		0.6
(-1.8)
A 		0.5
(-3)
A 		1
B 1
(-1.3)
A 		2.3
(3)
A 		1.1
(-1)
A 		0.5
(-3)
A 		2
C 0.9
(-2.5)
A 		1.9
(3)
A 		0.8
(-3)
A 		0.9
(-2.5)
A 		1
avr 0.9
(-1.4) 		1.7
(3) 		0.8
(-1.9) 		0.6
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 0.5
(-2.9)
A 		3.6
(0.8)
A 		0.4
(-3)
A 		1
(-2.3)
A 		1.9
(-1.2)
A 		5.5
(3)
A 		5
B 1.6
(-2.8)
A 		2.7
(-2.4)
A 		1
(-3)
A 		0.9
(-3)
A 		19.8
(3)
A 		18.7
(2.7)
A 		19
C 1
(-2.9)
A 		0.8
(-2.9)
A 		0.5
(-3)
A 		0.6
(-3)
A 		19.8
(1)
A 		29.8
(3)
A 		29
avr 1
(-2.9) 		2.4
(-2.4) 		0.6
(-3) 		0.8
(-2.9) 		13.8
(1.6) 		18
(3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189199_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10621
Sequence Type Exemplar sequence
Representative Public ID ZK354.2 NCBI
Target Description ZK354.2 /REP_DB=WormBase Gene ID /WP=CE15314 /TR=P91571 /GB=AAB42257.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171335(11), NM_171336(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171335 NCBI Caenorhabditis elegans ZK354.2b (ZK354.2) mRNA, complete cds. 11/11 None
NM_171336 NCBI Caenorhabditis elegans ZK354.2a (ZK354.2) mRNA, complete cds. 11/11 None
SNAP00000006014 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5313471:5315168:1 11/11 None
GENEFINDER00000006016 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5313471:5315168:1 11/11 None
ZK354.2b ENSEMBL cdna:known chromosome:CEL140:IV:5313471:5315514:1 gene:ZK354.2 11/11 None
ZK354.2a ENSEMBL cdna:known chromosome:CEL140:IV:5313471:5315168:1 gene:ZK354.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5313476-5315174(+) 96.59 100.0
Public Domain and Genome References
Ensembl ZK354.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM97988 Hypothetical protein ZK354.2b [Caenorhabditis elegans] ref|NP_741394.1| ZK354.2b [Caenorhabditis elegans] 0.0
blast AAB42257 Hypothetical protein ZK354.2a [Caenorhabditis elegans] ref|NP_741395.1| ZK354.2a [Caenorhabditis elegans] 0.0
blast AAK29951 Hypothetical protein Y65B4A.9 [Caenorhabditis elegans] ref|NP_490767.1| Y65B4A.9 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 8.7E-17
Sequence
Target Sequence 
>C. ELEGANS:189199_S_AT
cgtgcttcacttgaaacgtcggatcactgcaccaatggtcgcggcagtttggagaacaac
tcctgagaccactgctgggttctttgatcttctgaccgtcatccgtgagccgaaggatgt
cgcagagtttgtggagtacgatcgtatcgctgatggaattcagaagttgtttgagaagtc
gaagtcgaatactcaagagccaccagattgggattgtatgacggattacaagggtcccgg
gtaccagcaggtaccgatgattgcacctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTGCTTCACTTGAAACGTCGGATC 70 245 744 Antisense
TTGAAACGTCGGATCACTGCACCAA 193 703 754 Antisense
ATCACTGCACCAATGGTCGCGGCAG 464 27 766 Antisense
GCACCAATGGTCGCGGCAGTTTGGA 113 315 772 Antisense
TGGTCGCGGCAGTTTGGAGAACAAC 188 553 779 Antisense
TGGAGAACAACTCCTGAGACCACTG 414 549 793 Antisense
ACCGTCATCCGTGAGCCGAAGGATG 255 89 838 Antisense
TCCGTGAGCCGAAGGATGTCGCAGA 432 595 845 Antisense
GCCGAAGGATGTCGCAGAGTTTGTG 78 283 852 Antisense
GTCCCGGGTACCAGCAGGTACCGAT 287 259 977 Antisense
AGCAGGTACCGATGATTGCACCTCC 546 77 989 Antisense
Sequence Source Affymetrix Proprietary Database