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[189193_at] Protein kinase

Description F59A6.4 /REP_DB=WormBase Gene ID /WP=CE01982 /TR=Q21026 /GB=AAK31524.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster 6grp_total > 88      4grp_total > 04      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 4.2
(-3)
A 		5.6
(-2.8)
A 		6.6
(-2.7)
A 		57.8
(3)
A 		54
B 6.3
(-0.1)
A 		2.4
(-2.5)
A 		1.6
(-3)
A 		11.4
(3)
A 		10
C 17.8
(-0.9)
A 		3.3
(-2.8)
A 		1.7
(-3)
A 		46.7
(3)
A 		45
avr 9.4
(-2) 		3.8
(-2.9) 		3.3
(-3) 		38.6
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 50.2
(1.6)
A 		3.5
(-3)
A 		45.8
(1.1)
A 		3
(-3)
A 		45.3
(1.1)
A 		65.1
(3)
A 		62
B 1.1
(-3)
A 		1.4
(-3)
A 		10.5
(-2.1)
A 		28.2
(-0.5)
A 		48.5
(1.3)
A 		66.9
(3)
P 		66
C 9
(-2.3)
A 		17.5
(-1.4)
A 		1.3
(-3)
A 		2.3
(-2.9)
A 		36.4
(0.4)
A 		62.9
(3)
P 		62
avr 20.1
(-1.7) 		7.5
(-3) 		19.2
(-1.8) 		11.2
(-2.6) 		43.4
(0.7) 		65
(3) 		58
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 1.5
(-2.8)
A 		1.2
(-3)
A 		1.7
(-2.6)
A 		8.5
(3)
A 		7
B 2
(1.8)
A 		0.6
(-2.4)
A 		0.4
(-3)
A 		2.4
(3)
A 		2
C 5.9
(1.4)
A 		1
(-2.7)
A 		0.6
(-3)
A 		7.9
(3)
A 		7
avr 3.1
(-0.6) 		0.9
(-3) 		0.9
(-3) 		6.3
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 9.3
(0.4)
A 		1.2
(-2.8)
A 		10
(0.7)
A 		0.8
(-3)
A 		13.7
(2.1)
A 		15.9
(3)
A 		15
B 0.4
(-3)
A 		0.5
(-3)
A 		2.5
(-2.5)
A 		7.5
(-1.3)
A 		19.7
(1.7)
A 		25
(3)
P 		25
C 3.3
(-2.1)
A 		5.3
(-1.6)
A 		0.3
(-3)
A 		0.5
(-2.9)
A 		11.8
(0.3)
A 		21.1
(3)
P 		21
avr 4.3
(-2.3) 		2.3
(-3) 		4.3
(-2.3) 		2.9
(-2.8) 		15.1
(1.2) 		20.7
(3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189193_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4321
Sequence Type Exemplar sequence
Representative Public ID F59A6.4 NCBI
Target Description F59A6.4 /REP_DB=WormBase Gene ID /WP=CE01982 /TR=Q21026 /GB=AAK31524.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062521(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062521 NCBI Caenorhabditis elegans F59A6.4 (F59A6.4) mRNA, complete cds. 11/11 None
SNAP00000007647 ENSEMBL cdna:SNAP chromosome:CEL140:II:5005122:5007996:1 11/11 None
GENEFINDER00000007658 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5005122:5007996:1 11/11 None
F59A6.4 ENSEMBL cdna:known chromosome:CEL140:II:5005076:5008034:1 gene:F59A6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5005119-5007994(+) 100.0 100.0
Public Domain and Genome References
Gene Title Protein kinase
Ensembl F59A6.4
Entrez Gene 173865 Entrez gene
SwissProt Q21026 EMBL-EBI
WormBase CE01982 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31524 Hypothetical protein F59A6.4 [Caenorhabditis elegans] ref|NP_494922.1| F59A6.4 [Caenorhabditis elegans] 0.0
blast NP_494824 T05A7.6 [Caenorhabditis elegans] gb|AAA81115.1| Hypothetical protein T05A7.6 [Caenorhabditis elegans] 0.0
blast CAE73426 Hypothetical protein CBG20869 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 0.27
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-6
Pfam IPR000719 EMBL-EBI Protein kinase 3.1E-5
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-6
Pfam IPR000719 EMBL-EBI Protein kinase 3.1E-5
Sequence
Target Sequence 
>C. ELEGANS:189193_AT
ggatcttcattcgattggttatctccatcgagatatcaagccagcaaactatgcagttgg
actggaggatcgtgaagatactgtctacatgcttgattttggaatcgccaagttgtatgt
ggacgagaatggagaacacaaagtgaagagaaagaaagtaaagttcctgggaacattgag
atatgcttgtcgggcatgcatgatgcaacaggaacaaggacggaaggatgatttggagac
ttggatctacttggtgtttgatttgatggatgaatcccatggaatgccttggagatcttt
gtgcagtcccaaagagatcttgaagtcgaaaaacgaatttttcactaatttcgatagttc
ttccgtctctgcgactctgaaacgtctgaaaggattggtatcatatgttgataacatgca
atacgagacaactcctgattacgactacattatcaacttccttaagacaactgccacaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATCTTCATTCGATTGGTTATCTC 125 513 1677 Antisense
CTCCATCGAGATATCAAGCCAGCAA 338 235 1699 Antisense
GCAGTTGGACTGGAGGATCGTGAAG 56 311 1729 Antisense
ATACTGTCTACATGCTTGATTTTGG 99 23 1754 Antisense
GGATGAATCCCATGGAATGCCTTGG 30 503 1944 Antisense
GCCTTGGAGATCTTTGTGCAGTCCC 665 281 1962 Antisense
TAATTTCGATAGTTCTTCCGTCTCT 148 635 2022 Antisense
GTCTCTGCGACTCTGAAACGTCTGA 315 471 2041 Antisense
GAGACAACTCCTGATTACGACTACA 464 391 2101 Antisense
TACGACTACATTATCAACTTCCTTA 147 649 2116 Antisense
ACTTCCTTAAGACAACTGCCACAGA 340 101 2132 Antisense
Sequence Source Affymetrix Proprietary Database