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[189186_at] dehydrogenase

Description T25G12.7 /REP_DB=WormBase Gene ID /WP=CE07544 /TR=Q22785 /GB=AAC69022.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 64      6grp_total > 63      4grp_total > 61      6grp_highest_sub > 03_04      6grp_cgc > 76      6grp_high1_sub > 03_04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 706
1 2 3 4 range
A 797.5
(3)
P 		513.3
(-0.1)
P 		624.4
(1.1)
P 		246.8
(-3)
P 		551
B 848.7
(3)
P 		623.8
(1)
P 		637.6
(1.1)
P 		187
(-3)
P 		662
C 749.1
(3)
P 		604.4
(1.6)
P 		530.2
(0.8)
P 		142.8
(-3)
P 		606
avr 798.4
(3) 		580.5
(0.8) 		597.4
(1) 		192.2
(-3) 		606

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 557
1 2 3 4 5 6 range
A 721.1
(-3)
P 		839.7
(-1.6)
P 		1151.5
(1.9)
P 		1246.5
(3)
P 		826.2
(-1.8)
P 		966.2
(-0.2)
P 		525
B 755
(-3)
P 		793.4
(-2.4)
P 		1164
(3)
P 		907.6
(-0.8)
P 		1001.2
(0.6)
P 		963.8
(0.1)
P 		409
C 834.1
(-2)
P 		741.6
(-3)
P 		1277.6
(3)
P 		1178.7
(1.9)
P 		925.1
(-0.9)
P 		882.2
(-1.4)
P 		536
avr 770.1
(-3) 		791.6
(-2.7) 		1197.7
(3) 		1110.9
(1.8) 		917.5
(-0.9) 		937.4
(-0.7) 		428
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 266
1 2 3 4 range
A 290.4
(3)
P 		111.4
(-1.2)
P 		163.2
(-0)
P 		36.1
(-3)
P 		254
B 270.6
(3)
P 		154.1
(-0)
P 		147.8
(-0.2)
P 		39.8
(-3)
P 		231
C 248
(3)
P 		173.8
(1)
P 		184.8
(1.3)
P 		24.1
(-3)
P 		224
avr 269.7
(3) 		146.4
(-0.1) 		165.3
(0.4) 		33.3
(-3) 		236

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 274
1 2 3 4 5 6 range
A 132.9
(-3)
P 		298.6
(2.4)
P 		250.8
(0.9)
P 		316.2
(3)
P 		249.3
(0.8)
P 		235.2
(0.3)
P 		183
B 262.7
(-2.2)
P 		264.1
(-2.1)
P 		282.7
(-1.5)
P 		240.4
(-3)
P 		406.8
(3)
P 		359.4
(1.3)
P 		166
C 309.2
(2)
P 		226.3
(-3)
P 		325.2
(3)
P 		237
(-2.4)
P 		299.3
(1.4)
P 		295.3
(1.2)
P 		99
avr 234.9
(-3) 		263
(-1) 		286.2
(0.7) 		264.5
(-0.9) 		318.5
(3) 		296.6
(1.4) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189186_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17893
Sequence Type Exemplar sequence
Representative Public ID T25G12.7 NCBI
Target Description T25G12.7 /REP_DB=WormBase Gene ID /WP=CE07544 /TR=Q22785 /GB=AAC69022.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078392(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078392 NCBI Caenorhabditis elegans T25G12.7 (dehydrogenase) mRNA, complete cds. 11/11 None
SNAP00000027873 ENSEMBL cdna:SNAP chromosome:CEL140:X:17233783:17235748:-1 11/11 None
GENEFINDER00000027881 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17234656:17237450:-1 11/11 None
T25G12.7 ENSEMBL cdna:known chromosome:CEL140:X:17230751:17235748:-1 gene:T25G12.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17234655-17235748(-) 100.0 100.0
Public Domain and Genome References
Gene Title dehydrogenase
Gene Symbol dhs-30
Ensembl T25G12.7
Entrez Gene 181761 Entrez gene
SwissProt Q65ZI3 EMBL-EBI
WormBase CE37275 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.20165.1.S1_AT similar to CG7601-PA cfa
CANINE_2:CFAAFFX.28324.1.S1_S_AT similar to CG7601-PA cfa
DROSOPHILA_2:1639727_AT dm
DROSGENOME1:152047_AT dm
CHICKEN:GGA.7346.1.S1_AT similar to DKFZP566O084 protein gga
HG-U133_PLUS_2:220690_S_AT DKFZP566O084 protein hs
HG-U133A:220690_S_AT DKFZP566O084 protein hs
HG-U133A_2:220690_S_AT DKFZP566O084 protein hs
HU35KSUBA:RC_AA457140_AT DKFZP566O084 protein hs
U133_X3P:G7661657_3P_S_AT DKFZP566O084 protein hs
HG-U95B:52920_AT DKFZP566O084 protein hs
HG-U133_PLUS_2:239191_AT DKFZP566O084 protein hs
HG-U133B:239191_AT DKFZP566O084 protein hs
HG-U95C:61443_AT DKFZP566O084 protein hs
HU35KSUBC:RC_R93195_AT DKFZP566O084 protein hs
HU35KSUBD:RC_AA450011_AT DKFZP566O084 protein hs
U133_X3P:HS.99233.0.A1_3P_AT DKFZP566O084 protein hs
MOUSE430_2:1424869_AT cDNA sequence BC003479 mm
MOE430A:1424869_AT cDNA sequence BC003479 mm
MOUSE430A_2:1424869_AT cDNA sequence BC003479 mm
MU11KSUBA:AA733303_S_AT cDNA sequence BC003479 mm
MG-U74AV2:103442_AT cDNA sequence BC003479 mm
MU19KSUBA:TC20250_AT CDNA sequence BC003479 (BC003479), mRNA mm
MU19KSUBB:TC33015_AT CDNA sequence BC003479 (BC003479), mRNA mm
RG-U34A:RC_H33750_AT similar to DKFZP566O084 protein (predicted) rn
RAE230A:1374617_AT similar to DKFZP566O084 protein (predicted) rn
RAT230_2:1374617_AT similar to DKFZP566O084 protein (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAU05548 Dehydrogenases, short chain protein 30 [Caenorhabditis elegans] ref|NP_510793.2| T25G12.7 [Caenorhabditis elegans] 1.0E-171
blast AAC69018 Hypothetical protein T25G12.2 [Caenorhabditis elegans] ref|NP_510795.1| T25G12.2 [Caenorhabditis elegans] 1.0E-145
blast CAE69687 Hypothetical protein CBG15942 [Caenorhabditis briggsae] 1.0E-143
blast T34378 hypothetical protein T25G12.7 - Caenorhabditis elegans 1.0E-139
blast T34378 hypothetical protein T25G12.7 - Caenorhabditis elegans 1.0E-132
blast AAU05548 Dehydrogenases, short chain protein 30 [Caenorhabditis elegans] ref|NP_510793.2| T25G12.7 [Caenorhabditis elegans] 1.0E-131
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.2E-39
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 6.3E-39
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 6.3E-39
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000027881 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:189186_AT
ttacaaacgcggtgcccaggtgatccttcttgcgagaagcacagaaaagctcaaggagat
ctgcgaggagctcaaggaaaccttcccgctcaaccagaacgagccgatttactactactt
cgacatcaccgattcagagcaagcaccgtgggctgagatcccacgagtcgacattctcat
caacaacgccggcatgtcaaatcgcggatcatgccaggatacaactatggaaattcacag
acaagccatggagaccaactactttggacatgtgcatgtcacccaagctttactctcaaa
gttgagcccagatggttgtattgttgtcacaagtagcattcaaggaaaagtggcaattcc
gtacagaggatcatatggagccagtaaacatgcacttcaaggatactttgattgtcttcg
cgccgagcacaagaacctgcacattttggtggtctccgctggatacatcaacactggttt
tggatcgagagctttggatccctctggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTACAAACGCGGTGCCCAGGTGATC 89 683 213 Antisense
GAGGAGCTCAAGGAAACCTTCCCGC 237 403 277 Antisense
TTCCCGCTCAACCAGAACGAGCCGA 315 695 295 Antisense
GCCGATTTACTACTACTTCGACATC 554 283 315 Antisense
GACATCACCGATTCAGAGCAAGCAC 513 369 334 Antisense
GAGCAAGCACCGTGGGCTGAGATCC 121 391 349 Antisense
TCCCACGAGTCGACATTCTCATCAA 355 599 371 Antisense
CAACAACGCCGGCATGTCAAATCGC 394 189 393 Antisense
GTCAAATCGCGGATCATGCCAGGAT 514 463 408 Antisense
ATGTGCATGTCACCCAAGCTTTACT 23 49 482 Antisense
TCGAGAGCTTTGGATCCCTCTGGAA 484 601 697 Antisense
Sequence Source Affymetrix Proprietary Database