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[189184_at] choline kinase

Description F22F7.5 /REP_DB=WormBase Gene ID /WP=CE17695 /TR=Q9GZF3 /GB=AAF99916.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=choline kinase
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 11      6grp_highest_nonorm > 42      6grp_total > 23      4grp_total > 56      6grp_cgc > 60      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 471
1 2 3 4 range
A 315.6
(-0.6)
P 		443.5
(1.4)
P 		549.6
(3)
P 		154.2
(-3)
A 		395
B 295.5
(-1)
P 		345
(-0.4)
P 		612.6
(3)
P 		142
(-3)
A 		471
C 256.3
(-0.3)
P 		320.7
(1.3)
P 		391.9
(3)
P 		146.5
(-3)
A 		245
avr 289.1
(-0.7) 		369.7
(0.6) 		518
(3) 		147.6
(-3) 		370

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 766
1 2 3 4 5 6 range
A 822.7
(2)
P 		861.2
(2.6)
P 		684.8
(-0.3)
P 		882.8
(3)
P 		520.3
(-3)
P 		562.8
(-2.3)
P 		363
B 1061.8
(3)
P 		902.4
(1.2)
P 		852.2
(0.7)
P 		735.5
(-0.7)
P 		591.8
(-2.3)
P 		525.9
(-3)
P 		536
C 1033.8
(1.6)
P 		813
(-0.1)
P 		1208.4
(3)
P 		789.9
(-0.3)
P 		546.4
(-2.2)
P 		442.4
(-3)
P 		766
avr 972.8
(3) 		858.9
(1.5) 		915.1
(2.3) 		802.7
(0.8) 		552.8
(-2.4) 		510.4
(-3) 		462
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 114.9
(1.6)
P 		96.3
(0.7)
P 		143.7
(3)
P 		22.6
(-3)
A 		121
B 94.2
(0.4)
P 		85.2
(-0)
P 		141.9
(3)
P 		30.3
(-3)
A 		112
C 84.8
(0.2)
P 		92.2
(0.6)
P 		136.6
(3)
P 		24.7
(-3)
A 		112
avr 98
(0.8) 		91.2
(0.4) 		140.7
(3) 		25.9
(-3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 246
1 2 3 4 5 6 range
A 151.6
(-2.5)
P 		306.3
(3)
P 		149.1
(-2.6)
P 		223.9
(0.1)
P 		157
(-2.3)
P 		137
(-3)
P 		169
B 369.5
(3)
P 		300.4
(0.6)
P 		206.9
(-2.6)
P 		194.8
(-3)
P 		240.4
(-1.4)
P 		196.1
(-3)
P 		175
C 383.2
(3)
P 		248.1
(-0.4)
P 		307.5
(1.1)
P 		158.9
(-2.7)
P 		176.8
(-2.3)
P 		148.1
(-3)
P 		235
avr 301.4
(3) 		284.9
(2.3) 		221.2
(-0.4) 		192.5
(-1.6) 		191.4
(-1.7) 		160.4
(-3) 		141
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189184_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15776
Sequence Type Exemplar sequence
Representative Public ID F22F7.5 NCBI
Target Description F22F7.5 /REP_DB=WormBase Gene ID /WP=CE17695 /TR=Q9GZF3 /GB=AAF99916.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=choline kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071172(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071172 NCBI Caenorhabditis elegans Choline Kinase B family member (ckb-4) (ckb-4) mRNA, complete cds. 11/11 None
SNAP00000032278 ENSEMBL cdna:SNAP chromosome:CEL140:V:2106328:2109128:-1 11/11 None
GENEFINDER00000032286 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2106328:2109885:-1 11/11 None
F22F7.5 ENSEMBL cdna:known chromosome:CEL140:V:2106328:2109128:-1 gene:F22F7.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2106327-2109128(-) 100.0 100.0
Public Domain and Genome References
Gene Title choline kinase
Gene Symbol ckb-4
Ensembl F22F7.5
Entrez Gene 184864 Entrez gene
SwissProt Q9GZF3 EMBL-EBI
WormBase CE17695 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99916 Choline kinase b protein 4 [Caenorhabditis elegans] ref|NP_503573.1| Choline Kinase B family member (ckb-4) [Caenorhabditis elegans] 0.0
blast CAE65186 Hypothetical protein CBG10059 [Caenorhabditis briggsae] 1.0E-156
blast CAE65186 Hypothetical protein CBG10059 [Caenorhabditis briggsae] 1.0E-155
blast CAE65186 Hypothetical protein CBG10059 [Caenorhabditis briggsae] 1.0E-150
blast CAE65188 Hypothetical protein CBG10061 [Caenorhabditis briggsae] 1.0E-116
Protein Domains
Database ID Description E-Value
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.3E-104
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 4.1E-97
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.3E-104
Sequence
Target Sequence 
>C. ELEGANS:189184_AT
aagttctcaaaatgtgcccacactctgtgaagtatgatagcatgccgagtgagctcaccg
tggagcaaatcgaacaggaaattgctacttttgagagatggtgcaaagttttcgatgaga
cgattgttttcagtcataacgatttggcgccactgaacgttctcgagctgaacgacacca
aggaaattgtgtttatagattttgagtattcttcttacaattggagaggttttgacttgt
gcatgttcctatgcgaaaacgcctttgactatcgggatccaactccgccaggtctcgtca
ttgatcaggattatatggaaaatcatccgaacctccagatcttttgtgaagcatacattg
atacgcagtacaagatgaaaaccgctaacccgaagcaaaaattcccactgactgaagggc
gtgccgctgaagtggagtctctcatgttggagtgcaaatttttcattccactggtcaata
tgttctgggcagtatggtcacttaggcaacatttggcaaaatacgaaatcgggatggatt
tggatgttatcgccagtgaccgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTTCTCAAAATGTGCCCACACTC 6 157 533 Antisense
GATAGCATGCCGAGTGAGCTCACCG 594 423 568 Antisense
AGTCATAACGATTTGGCGCCACTGA 666 61 664 Antisense
GCCACTGAACGTTCTCGAGCTGAAC 111 279 681 Antisense
GGTTTTGACTTGTGCATGTTCCTAT 169 507 760 Antisense
AAACGCCTTTGACTATCGGGATCCA 93 121 789 Antisense
GCCAGGTCTCGTCATTGATCAGGAT 184 281 819 Antisense
TCCGAACCTCCAGATCTTTTGTGAA 177 595 858 Antisense
ATTCCCACTGACTGAAGGGCGTGCC 406 7 933 Antisense
GCCGCTGAAGTGGAGTCTCTCATGT 657 283 955 Antisense
TTGGATGTTATCGCCAGTGACCGCT 241 705 1072 Antisense
Sequence Source Affymetrix Proprietary Database