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[189176_at] cytochrome P450

Description C36A4.1 /REP_DB=WormBase Gene ID /WP=CE03070 /TR=Q27477 /GB=CAA91268.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      6grp_highest_nonorm > 52      6grp_total > 62      4grp_total > 19      6grp_highest_sub > 03_04      6grp_cgc > 84      6grp_high1_sub > 03      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 37.3
(-3)
A 		43.4
(-2.6)
A 		41
(-2.8)
A 		131
(3)
A 		94
B 44.2
(1.2)
A 		57.8
(3)
A 		42
(0.9)
A 		12.4
(-3)
A 		45
C 52.5
(-1.5)
A 		76
(0.6)
A 		36.2
(-3)
A 		103.4
(3)
A 		67
avr 44.7
(-2.3) 		59.1
(-0.3) 		39.7
(-3) 		82.3
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 778
1 2 3 4 5 6 range
A 293
(-3)
P 		497.9
(-1.4)
P 		1070.9
(3)
P 		1025.8
(2.7)
P 		543.9
(-1.1)
P 		303.9
(-2.9)
P 		778
B 457.4
(-2)
P 		491.3
(-1.5)
P 		848.6
(3)
P 		796.4
(2.3)
P 		788.8
(2.2)
P 		375.6
(-3)
P 		473
C 369.1
(-2.5)
P 		732.5
(0.9)
P 		901.3
(2.4)
P 		963.4
(3)
P 		570.6
(-0.6)
P 		314.6
(-3)
P 		649
avr 373.2
(-2.6) 		573.9
(-0.6) 		940.3
(3) 		928.5
(2.9) 		634.4
(-0) 		331.4
(-3) 		609
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 13.6
(-0.4)
A 		9.4
(-3)
A 		10.7
(-2.2)
A 		19.2
(3)
A 		10
B 14.1
(2.9)
A 		14.3
(3)
A 		9.7
(0.6)
A 		2.7
(-3)
A 		12
C 17.4
(0.1)
A 		21.9
(3)
A 		12.6
(-3)
A 		17.4
(0.1)
A 		9
avr 15
(2.7) 		15.2
(3) 		11
(-3) 		13.1
(0) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 54
(-3)
P 		177.1
(0.6)
P 		233.2
(2.2)
P 		260.2
(3)
P 		164.1
(0.2)
P 		74
(-2.4)
P 		206
B 159.2
(-2.4)
P 		163.5
(-2.2)
P 		206.1
(-0.8)
P 		210.9
(-0.6)
P 		320.5
(3)
P 		140
(-3)
P 		181
C 136.8
(-1.5)
P 		223.5
(2.7)
P 		229.4
(3)
P 		193.7
(1.3)
P 		184.6
(0.8)
P 		105.3
(-3)
P 		124
avr 116.7
(-2.5) 		188
(1.2) 		222.9
(3) 		221.6
(2.9) 		223.1
(3) 		106.4
(-3) 		117
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189176_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7829
Sequence Type Exemplar sequence
Representative Public ID C36A4.1 NCBI
Target Description C36A4.1 /REP_DB=WormBase Gene ID /WP=CE03070 /TR=Q27477 /GB=CAA91268.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065374(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065374 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-25A1) (cyp-25A1) mRNA, complete cds. 11/11 None
C36A4.1 ENSEMBL cdna:known chromosome:CEL140:III:3832626:3834499:1 gene:C36A4.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028390 8/11 Cross Hyb Matching Probes None
GENEFINDER00000028406 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3832625-3834499(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-25A1
Ensembl C36A4.1
Entrez Gene 183247 Entrez gene
SwissProt Q27477 EMBL-EBI
WormBase CE37711 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1633947_AT dm
DROSGENOME1:150732_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91268 Hypothetical protein C36A4.1 [Caenorhabditis elegans] ref|NP_497775.2| CYtochrome P450 family member (cyp-25A1) [Caenorhabditis elegans] 0.0
blast CAA91267 Hypothetical protein C36A4.2 [Caenorhabditis elegans] ref|NP_497776.2| CYtochrome P450 family member (cyp-25A2) [Caenorhabditis elegans] 0.0
blast A88414 protein C36A4.3 [imported] - Caenorhabditis elegans 0.0
Protein Families
Method ID Description E-Value
p450 CP15_CHICK Animalia; CYP1A5; chi; CP15_CHICK; Gallus gallus; P79761 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.5E-29
Pfam IPR001128 EMBL-EBI Cytochrome P450 7.4E-64
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497775.2 1 2-24
C36A4.1 1 2-24
Sequence
Target Sequence 
>C. ELEGANS:189176_AT
gaaactttgagattctatccaccacatttcagtttcatccggcgtctttgtcgagaggat
attacgattcgtggccaattctatccgaagggagcaattgtagtttgcctgccacacact
gtccaccgtaacccagaaaattgggattcccccgaagaattccatccggaaagatttgag
aattgggaagaaaagagtagctcattgaaatggattccatttggtgttggaccaagatat
tgtgttggaatgagatttgctgaaatggaatttaaaaccacaattgtcaagttactggat
acttttgaactgaaacaattcgaaggagaagctgatttgattccagattgtaatggagtt
attatgagaccaaatgatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACTTTGAGATTCTATCCACCAC 219 357 1135 Antisense
AGATTCTATCCACCACATTTCAGTT 46 65 1144 Antisense
TATCCACCACATTTCAGTTTCATCC 143 659 1150 Antisense
TCATCCGGCGTCTTTGTCGAGAGGA 144 623 1169 Antisense
CCGGCGTCTTTGTCGAGAGGATATT 601 263 1173 Antisense
CGAGAGGATATTACGATTCGTGGCC 122 251 1186 Antisense
TACGATTCGTGGCCAATTCTATCCG 256 649 1197 Antisense
GTGGCCAATTCTATCCGAAGGGAGC 330 491 1205 Antisense
GGGAGCAATTGTAGTTTGCCTGCCA 326 495 1224 Antisense
GCAATTGTAGTTTGCCTGCCACACA 431 323 1228 Antisense
GAGTTATTATGAGACCAAATGATCC 537 397 1490 Antisense
Sequence Source Affymetrix Proprietary Database