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[189173_s_at]

Description F28C6.4 /REP_DB=WormBase Gene ID /WP=CE03275 /TR=Q19870 /GB=CAA92675.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Yeast YJ10 like
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 77      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 82      6grp_total > 77      4grp_total > 38      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 413.1
(3)
P 		298
(-1.9)
P 		272.5
(-3)
P 		353
(0.4)
P 		141
B 387.3
(3)
P 		292.8
(-0.3)
P 		213
(-3)
P 		228.6
(-2.5)
P 		174
C 360.2
(3)
P 		283.2
(-3)
P 		296.4
(-2)
P 		321.3
(-0)
P 		77
avr 386.9
(3) 		291.3
(-1.5) 		260.6
(-3) 		301
(-1.1) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 256
1 2 3 4 5 6 range
A 260.5
(-2.3)
P 		323
(-0.7)
P 		233.2
(-3)
P 		269.9
(-2.1)
P 		382.6
(0.8)
P 		471.8
(3)
P 		239
B 357.7
(0)
P 		304.1
(-1.9)
P 		274.6
(-3)
P 		362
(0.2)
P 		336.2
(-0.8)
P 		440
(3)
P 		165
C 224.2
(-3)
P 		362.5
(0.2)
P 		317.9
(-0.8)
P 		303.6
(-1.1)
P 		232.4
(-2.8)
P 		480
(3)
P 		256
avr 280.8
(-2.8) 		329.9
(-1.3) 		275.2
(-3) 		311.8
(-1.8) 		317.1
(-1.7) 		463.9
(3) 		189
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 150.4
(3)
P 		64.7
(-2.2)
P 		71.2
(-1.8)
P 		51.7
(-3)
P 		99
B 123.5
(3)
P 		72.3
(-1.1)
P 		49.3
(-3)
P 		48.7
(-3)
P 		75
C 119.2
(3)
P 		81.4
(-0.5)
P 		103.3
(1.5)
P 		54.2
(-3)
P 		65
avr 131
(3) 		72.8
(-1.4) 		74.6
(-1.3) 		51.5
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 48
(-3)
P 		114.9
(2.9)
P 		50.8
(-2.8)
P 		68.4
(-1.2)
P 		115.5
(3)
P 		114.9
(2.9)
P 		68
B 124.5
(0.6)
P 		101.2
(-0.9)
P 		66.7
(-3)
P 		95.9
(-1.2)
P 		136.6
(1.3)
P 		164.1
(3)
P 		97
C 83.1
(-1.7)
P 		110.6
(-0)
P 		80.9
(-1.8)
P 		61.1
(-3)
P 		75.2
(-2.2)
P 		160.7
(3)
P 		100
avr 85.2
(-1.6) 		108.9
(0.2) 		66.1
(-3) 		75.1
(-2.3) 		109.1
(0.2) 		146.6
(3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189173_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3695
Sequence Type Exemplar sequence
Representative Public ID F28C6.4 NCBI
Target Description F28C6.4 /REP_DB=WormBase Gene ID /WP=CE03275 /TR=Q19870 /GB=CAA92675.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Yeast YJ10 like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026952(11), NM_001026953(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026952 NCBI Caenorhabditis elegans F28C6.4a (F28C6.4) mRNA, complete cds. 11/11 None
NM_001026953 NCBI Caenorhabditis elegans F28C6.4b (F28C6.4) mRNA, complete cds. 11/11 None
SNAP00000038091 ENSEMBL cdna:SNAP chromosome:CEL140:II:8596222:8599396:-1 11/11 None
F28C6.4a ENSEMBL cdna:known chromosome:CEL140:II:8596222:8599144:-1 gene:F28C6.4 11/11 None
F28C6.4b.1 ENSEMBL cdna:known chromosome:CEL140:II:8596244:8599230:-1 gene:F28C6.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000038100 4/11 Cross Hyb Matching Probes None
F28C6.4b.3 7/11 Cross Hyb Matching Probes None
F28C6.4b.2 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8596218-8599452(-) 100.0 100.0
Public Domain and Genome References
Ensembl F28C6.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92675 Hypothetical protein F28C6.4a [Caenorhabditis elegans] ref|NP_001022123.1| F28C6.4a [Caenorhabditis elegans] 0.0
blast CAE48501 Hypothetical protein F28C6.4b [Caenorhabditis elegans] ref|NP_001022124.1| F28C6.4b [Caenorhabditis elegans] 0.0
blast CAE57572 Hypothetical protein CBG00550 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022123.1 7 13-32,400-422,523-542,590-612,619-641,661-683,704-726
NP_001022124.1 6 13-32,400-422,523-542,589-611,624-646,666-688
F28C6.4a 7 13-32,400-422,523-542,590-612,619-641,661-683,704-726
F28C6.4b.1 6 13-32,400-422,523-542,589-611,624-646,666-688
SNAP00000038091 5 54-76,551-573,580-602,622-644,664-686
Sequence
Target Sequence 
>C. ELEGANS:189173_S_AT
ttcattcggttatcaattctccctatctcatattcaaccggatcagtgctcgctggatgt
cttccattgctcctcgcaattgtaccagtatctcaaattttactgctcctagtcatatac
aatatgggaaaactgttctcaaagctgaatcttggaagtgtagaatttgtcacactgtgc
atgtcatctttcttctatacgggaaactcgaacagtttatcaacattatctttaacatca
gcttacgttggactttcgagttatcagccgatccttgttggacttcagctcatgctatat
acattctcgggtcccattttgttcctttgtggatgcaatgcttcatcatgcacatcattg
cttaatcatctcttctcctatcgaatctcatccatgtgcacatcacttatttgcctttac
atatttcaaaatcatcttttcatctggtctgtat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCATTCGGTTATCAATTCTCCCTA 82 689 1882 Antisense
TATTCAACCGGATCAGTGCTCGCTG 538 659 1912 Antisense
CTCCTCGCAATTGTACCAGTATCTC 200 235 1951 Antisense
TGTGCATGTCATCTTTCTTCTATAC 319 557 2057 Antisense
AGCCGATCCTTGTTGGACTTCAGCT 215 83 2147 Antisense
TATATACATTCTCGGGTCCCATTTT 366 659 2177 Antisense
GGTCCCATTTTGTTCCTTTGTGGAT 189 503 2191 Antisense
CATCATTGCTTAATCATCTCTTCTC 498 213 2234 Antisense
TCGAATCTCATCCATGTGCACATCA 344 601 2262 Antisense
GTGCACATCACTTATTTGCCTTTAC 362 481 2277 Antisense
AATCATCTTTTCATCTGGTCTGTAT 298 171 2311 Antisense
Sequence Source Affymetrix Proprietary Database