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[189171_at]

Description F32A5.1 /REP_DB=WormBase Gene ID /WP=CE28287 /TR=Q19953 /GB=AAK68355.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=oxidoreductase
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 51      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 33      6grp_total > 40      4grp_total > 59      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 562
1 2 3 4 range
A 1254.1
(-1.2)
P 		1514
(3)
P 		1407.6
(1.3)
P 		1142.1
(-3)
P 		372
B 1422.3
(1.9)
P 		1499.5
(3)
P 		1439.1
(2.2)
P 		1066.8
(-3)
P 		433
C 1366.1
(1.8)
P 		1344.5
(1.5)
P 		1471.3
(3)
P 		952.5
(-3)
P 		519
avr 1347.5
(1.4) 		1452.7
(3) 		1439.3
(2.8) 		1053.8
(-3) 		399

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 915
1 2 3 4 5 6 range
A 1574.9
(0.3)
P 		1788.1
(1.8)
P 		1914.5
(2.7)
P 		1952.1
(3)
P 		1297.1
(-1.6)
P 		1099.1
(-3)
P 		853
B 1401.6
(-1)
P 		1547
(-0)
P 		1985.8
(3)
P 		1703.2
(1)
P 		1378.2
(-1.2)
P 		1117.6
(-3)
P 		868
C 1408.4
(-0.3)
P 		1592.5
(1.1)
P 		1832.2
(3)
P 		1673.2
(1.7)
P 		1280.1
(-1.3)
P 		1070.4
(-3)
P 		762
avr 1461.6
(-0.3) 		1642.5
(1) 		1910.8
(3) 		1776.2
(2) 		1318.5
(-1.4) 		1095.7
(-3) 		815
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 352
1 2 3 4 range
A 456.6
(3)
P 		328.7
(0.3)
P 		368
(1.2)
P 		167.2
(-3)
P 		289
B 453.6
(3)
P 		370.3
(0.8)
P 		333.5
(-0.2)
P 		227.3
(-3)
P 		226
C 452.2
(2)
P 		386.5
(0.8)
P 		512.8
(3)
P 		160.6
(-3)
P 		352
avr 454.1
(3) 		361.8
(0.9) 		404.8
(1.9) 		185
(-3) 		269

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 368
1 2 3 4 5 6 range
A 290.3
(-2.6)
P 		635.9
(3)
P 		416.9
(-0.6)
P 		495.1
(0.7)
P 		391.4
(-1)
P 		267.6
(-3)
P 		368
B 487.7
(-0)
P 		514.9
(1.1)
P 		482.2
(-0.3)
P 		451.1
(-1.6)
P 		560
(3)
P 		416.8
(-3)
P 		143
C 522.1
(3)
P 		485.9
(1.8)
P 		466.3
(1.2)
P 		336.5
(-3)
P 		414.2
(-0.5)
P 		358.3
(-2.3)
P 		186
avr 433.4
(-0.4) 		545.6
(3) 		455.1
(0.3) 		427.6
(-0.6) 		455.2
(0.3) 		347.6
(-3) 		198
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189171_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5858
Sequence Type Exemplar sequence
Representative Public ID F32A5.1 NCBI
Target Description F32A5.1 /REP_DB=WormBase Gene ID /WP=CE28287 /TR=Q19953 /GB=AAK68355.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=oxidoreductase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063115
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000015817 ENSEMBL cdna:SNAP chromosome:CEL140:II:7256953:7258693:1 11/11 None
GENEFINDER00000015818 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7256123:7258693:1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_063115 5/11 Cross Hyb Matching Probes None
F32A5.8 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7253351-7258689(+) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T16235 hypothetical protein F32A5.1 - Caenorhabditis elegans 0.0
blast CAE67584 Hypothetical protein CBG13117 [Caenorhabditis briggsae] 1.0E-176
blast AAK68358 Hypothetical protein F32A5.8 [Caenorhabditis elegans] ref|NP_495516.2| F32A5.8 [Caenorhabditis elegans] 1.0E-129
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 8.9E-24
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 8.9E-24
Sequence
Target Sequence 
>C. ELEGANS:189171_AT
gacagtcgtctccagcaagaatagtttttgtttcctcagtttccagtagtcacactggtc
tgaaagcagagatgacaagaagtgagaaattgaacaagttgtgcccagagaatgcaaatg
agttctactacaagctctatgcttattcgaagatgtgccaagtacttactgcgtttaaaa
ttcacagagatgaatttgtgagtcacggaatcagtacctatgcaattcatccaggaacta
tgattggaacagacatttctcgaggatttggattccttggaaagttctggaacattcttt
cgaagccattcaccaaaagtcttgctcaaggagcagcaacaagtgtctactgtgttgctc
atccggaagcggcagagttttctggaaagttctgggaatcgtgttgggatgatgaaaaga
gtcttgatgcggacatagcaagagacatatcacttcaagatgaactatgggaacactcgg
aatgccttattgatgaatggttcaaaagtcaaaaaccgatatctacaattggtaatgatg
agagttcacctgccgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAGTCGTCTCCAGCAAGAATAGT 330 369 2195 Antisense
TTTCCTCAGTTTCCAGTAGTCACAC 608 669 2225 Antisense
GAGTTCTACTACAAGCTCTATGCTT 137 399 2314 Antisense
GATGTGCCAAGTACTTACTGCGTTT 477 407 2346 Antisense
GAGTCACGGAATCAGTACCTATGCA 43 399 2394 Antisense
GAACATTCTTTCGAAGCCATTCACC 198 351 2484 Antisense
GCCATTCACCAAAAGTCTTGCTCAA 579 281 2499 Antisense
ACAAGTGTCTACTGTGTTGCTCATC 407 113 2533 Antisense
GTTGCTCATCCGGAAGCGGCAGAGT 598 439 2548 Antisense
GGAACACTCGGAATGCCTTATTGAT 246 529 2664 Antisense
TAATGATGAGAGTTCACCTGCCGCT 53 637 2727 Antisense
Sequence Source Affymetrix Proprietary Database