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[189162_s_at] protein kinase

Description F23C8.8 /REP_DB=WormBase Gene ID /WP=CE20717 /TR=Q9TXI1 /GB=AAD03136.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 95      4grp_total > 32      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 26
(-0.5)
A 		10.4
(-3)
A 		47.6
(3)
A 		27.7
(-0.2)
A 		37
B 6.6
(-3)
A 		59.8
(3)
A 		40.4
(0.8)
A 		30
(-0.4)
A 		53
C 38.1
(-0.9)
A 		25
(-3)
A 		33.3
(-1.7)
A 		62.3
(3)
A 		37
avr 23.6
(-3) 		31.7
(-0.1) 		40.4
(3) 		40
(2.9) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 48.2
(0.4)
A 		7.7
(-3)
A 		9.2
(-2.9)
A 		52.9
(0.8)
A 		79.8
(3)
A 		70.6
(2.2)
P 		72
B 41.7
(-0.8)
A 		29.4
(-1.6)
A 		5.4
(-3)
A 		60.9
(0.3)
A 		106.4
(3)
P 		75.5
(1.2)
P 		101
C 3
(-3)
A 		4.6
(-2.9)
A 		23.4
(-1.9)
A 		2.3
(-3)
A 		77.4
(1)
P 		115.8
(3)
P 		114
avr 31
(-1.5) 		13.9
(-2.9) 		12.7
(-3) 		38.7
(-0.9) 		87.9
(3) 		87.3
(3) 		75
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 9.5
(1.3)
A 		2.2
(-3)
A 		12.4
(3)
A 		4.1
(-1.9)
A 		10
B 2.1
(-3)
A 		14.8
(3)
A 		9.4
(0.4)
A 		6.4
(-1)
A 		13
C 12.6
(3)
A 		7.2
(-3)
A 		11.6
(1.9)
A 		10.5
(0.7)
A 		5
avr 8.1
(-1.4) 		8.1
(-1.4) 		11.1
(3) 		7
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 8.9
(-1.1)
A 		2.7
(-2.8)
A 		2
(-3)
A 		13.4
(0.1)
A 		24.1
(3)
A 		17.2
(1.1)
P 		22
B 14.5
(-1.1)
A 		9.8
(-1.8)
A 		1.3
(-3)
A 		16.1
(-0.9)
A 		43.2
(3)
P 		28.1
(0.8)
P 		42
C 1.1
(-2.9)
A 		1.4
(-2.9)
A 		6
(-2.1)
A 		0.5
(-3)
A 		25.1
(0.9)
P 		38.8
(3)
P 		38
avr 8.2
(-1.9) 		4.6
(-2.7) 		3.1
(-3) 		10
(-1.5) 		30.8
(3) 		28
(2.4) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189162_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.86
Sequence Type Exemplar sequence
Representative Public ID F23C8.8 NCBI
Target Description F23C8.8 /REP_DB=WormBase Gene ID /WP=CE20717 /TR=Q9TXI1 /GB=AAD03136.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058575(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058575 NCBI Caenorhabditis elegans F23C8.8 (F23C8.8) mRNA, complete cds. 11/11 None
SNAP00000018136 ENSEMBL cdna:SNAP chromosome:CEL140:I:2429773:2432582:-1 11/11 None
GENEFINDER00000018149 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2429773:2432582:-1 11/11 None
F23C8.8 ENSEMBL cdna:known chromosome:CEL140:I:2429773:2432582:-1 gene:F23C8.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2429772-2432582(-) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl F23C8.8
Entrez Gene 171802 Entrez gene
SwissProt Q9TXI1 EMBL-EBI
WormBase CE20717 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD03136 Hypothetical protein F23C8.8 [Caenorhabditis elegans] ref|NP_490976.1| F23C8.8 [Caenorhabditis elegans] 1.0E-176
blast XP_395246 PREDICTED: similar to CG9222-PA [Apis mellifera] 2.0E-33
blast XP_787834 PREDICTED: similar to serine/threonine kinase 22A (spermiogenesis associated) (predicted) [Strongylocentrotus purpuratus] 5.0E-33
Protein Families
Method ID Description E-Value
hanks 2.2.3 CaMK Group; CaMK II KIN1/SNF1/Nim1; kin1+ 3.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.3E-45
Sequence
Target Sequence 
>C. ELEGANS:189162_S_AT
ggcacacttctcgagtggattctccagaagaaacgtttgaaagagcacccattagcagcc
acactattccgtcaactcatcgaagcgatcaattatcttcacaaaagaggaatagtacat
cgagatgtgaaattggaaaatattctaatcgacggaaatggggatattaagctcatcgat
ttcggatttgcaagacacattgaacgacgtgaacggtctcgatcattctgtggaactcag
ccctacacatgcccgcaaatcacaaaatatcggccttatgaagcctacgcggcggactat
tatgcttgtggaatagtgctttttacgatggtcactggaaaatggccgaacatgacagag
aacccaaacatttatccggaaggatatccaagtcgttcgtgtcgtaaactcgtgtcttca
ctgctggacgggaatgaaatgagccgtgctggatatgatgagtgcgtgaattccgagtgg
atgggggtgcatcctgtttggatgtttgcgaatcacgcgtatttttatgaagaggtccgt
ggcgaggatccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCACACTTCTCGAGTGGATTCTCC 393 535 334 Antisense
TGAAAGAGCACCCATTAGCAGCCAC 324 573 371 Antisense
CACTATTCCGTCAACTCATCGAAGC 425 197 395 Antisense
AACGGTCTCGATCATTCTGTGGAAC 629 143 545 Antisense
TTCTGTGGAACTCAGCCCTACACAT 489 693 559 Antisense
TATCGGCCTTATGAAGCCTACGCGG 278 659 601 Antisense
TACGCGGCGGACTATTATGCTTGTG 312 649 619 Antisense
AGGATATCCAAGTCGTTCGTGTCGT 389 57 714 Antisense
TTCGTGTCGTAAACTCGTGTCTTCA 381 693 729 Antisense
TGTCTTCACTGCTGGACGGGAATGA 323 557 746 Antisense
TGAAGAGGTCCGTGGCGAGGATCCC 84 575 861 Antisense
Sequence Source Affymetrix Proprietary Database