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[189158_at] Glyoxalase

Description C16C10.10 /REP_DB=WormBase Gene ID /WP=CE01490 /TR=SW:Q09253 /GB=CAA86748.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Glyoxalase
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 85      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 95      6grp_total > 75      4grp_total > 02      6grp_cgc > 57      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 683
1 2 3 4 range
A 306.9
(-2.7)
P 		297.9
(-2.8)
P 		275.5
(-3)
P 		826.4
(3)
P 		551
B 351.4
(-2.2)
P 		258.4
(-3)
P 		377.8
(-1.9)
P 		933.4
(3)
P 		675
C 346
(-2.8)
P 		323.9
(-3)
P 		361.4
(-2.6)
P 		941.5
(3)
P 		618
avr 334.8
(-2.6) 		293.4
(-3) 		338.2
(-2.6) 		900.4
(3) 		607

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 930
1 2 3 4 5 6 range
A 322.6
(-3)
P 		468
(-1.7)
P 		736.2
(0.8)
P 		707.3
(0.5)
P 		672.5
(0.2)
P 		976
(3)
P 		653
B 324.4
(-3)
P 		494.8
(-1.5)
P 		1028.9
(3)
P 		525.4
(-1.3)
P 		784.4
(0.9)
P 		972.4
(2.5)
P 		705
C 380.2
(-3)
P 		532.1
(-2)
P 		510
(-2.1)
P 		867.5
(0.4)
P 		803.8
(-0.1)
P 		1252.7
(3)
P 		873
avr 342.4
(-3) 		498.3
(-1.7) 		758.4
(0.4) 		700.1
(-0) 		753.6
(0.4) 		1067
(3) 		725
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 111.7
(2)
P 		64.7
(-3)
P 		72
(-2.2)
P 		121
(3)
P 		56
B 112.1
(-0.9)
P 		63.8
(-3)
P 		87.6
(-1.9)
P 		198.9
(3)
P 		135
C 114.5
(-1)
P 		93.1
(-3)
P 		126
(0)
P 		158.7
(3)
P 		66
avr 112.8
(-0.3) 		73.9
(-3) 		95.2
(-1.5) 		159.5
(3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 360
1 2 3 4 5 6 range
A 59.5
(-3)
P 		166.4
(0.6)
P 		160.3
(0.4)
P 		179.4
(1)
P 		202.9
(1.8)
P 		237.6
(3)
P 		178
B 112.9
(-3)
P 		164.7
(-1.8)
P 		249.8
(0.3)
P 		139.2
(-2.4)
P 		318.7
(1.9)
P 		362.6
(3)
P 		250
C 140.9
(-2.8)
P 		162.4
(-2.3)
P 		129.8
(-3)
P 		174.5
(-2.1)
P 		260.1
(-0.3)
P 		419.4
(3)
P 		290
avr 104.4
(-3) 		164.5
(-1.5) 		180
(-1.1) 		164.4
(-1.5) 		260.6
(1) 		339.9
(3) 		236
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189158_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7616
Sequence Type Exemplar sequence
Representative Public ID C16C10.10 NCBI
Target Description C16C10.10 /REP_DB=WormBase Gene ID /WP=CE01490 /TR=SW:Q09253 /GB=CAA86748.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Glyoxalase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065426(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065426 NCBI Caenorhabditis elegans C16C10.10 (Glyoxalase) mRNA, complete cds. 11/11 None
SNAP00000043768 ENSEMBL cdna:SNAP chromosome:CEL140:III:4153572:4154557:1 11/11 None
GENEFINDER00000043791 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4153572:4154557:1 11/11 None
C16C10.10 ENSEMBL cdna:known chromosome:CEL140:III:4153572:4154557:1 gene:C16C10.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4153571-4154557(+) 100.0 100.0
Public Domain and Genome References
Gene Title Glyoxalase
Gene Symbol tag-73
Ensembl C16C10.10
Entrez Gene 175530 Entrez gene
SwissProt Q09253 EMBL-EBI
WormBase CE01490 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264970_AT lactoylglutathione lyase, putative / glyoxalase I, putative at
ATGENOME1:20688_AT lactoylglutathione lyase, putative / glyoxalase I, putative at
CANINE:1592365_AT similar to CG1532-PA cfa
CANINE_2:CFA.9425.1.A1_AT similar to CG1532-PA cfa
CANINE_2:CFAAFFX.29198.1.S1_S_AT similar to CG1532-PA cfa
DROSOPHILA_2:1623959_AT dm
DROSGENOME1:142842_AT dm
HG-U133_PLUS_2:209092_S_AT chromosome 17 open reading frame 25 hs
HG-U133A:209092_S_AT chromosome 17 open reading frame 25 hs
HG-U133A_2:209092_S_AT chromosome 17 open reading frame 25 hs
HG-U95AV2:38667_AT chromosome 17 open reading frame 25 hs
U133_X3P:G12001995_3P_S_AT chromosome 17 open reading frame 25 hs
HG-U95E:80363_AT chromosome 17 open reading frame 25 hs
HG-U95E:72909_R_AT Chromosome 17 open reading frame 25 hs
MG-U74AV2:98613_AT RIKEN cDNA 2700085E05 gene mm
MOE430A:1428421_A_AT RIKEN cDNA 2700085E05 gene mm
MOUSE430A_2:1428421_A_AT RIKEN cDNA 2700085E05 gene mm
MOUSE430_2:1428421_A_AT RIKEN cDNA 2700085E05 gene mm
MG-U74BV2:116821_AT RIKEN cDNA 2700085E05 gene (2700085E05Rik), mRNA mm
MG-U74CV2:134711_AT RIKEN cDNA 2700085E05 gene (2700085E05Rik), mRNA mm
MU19KSUBB:TC28822_AT RIKEN cDNA 2700085E05 gene (2700085E05Rik), mRNA mm
MU19KSUBB:TC28822_G_AT RIKEN cDNA 2700085E05 gene (2700085E05Rik), mRNA mm
MU19KSUBC:TC40767_AT RIKEN cDNA 2700085E05 gene (2700085E05Rik), mRNA mm
Protein Similarities
Method ID Description E-Value
blast CAA86748 Hypothetical protein C16C10.10 [Caenorhabditis elegans] ref|NP_497827.1| C16C10.10 [Caenorhabditis elegans] sp|Q09253|YQ5A_CAEEL Hypothetical protein C16C10.10 in chromosome III 1.0E-162
blast CAE72872 Hypothetical protein CBG20174 [Caenorhabditis briggsae] 1.0E-141
blast XP_589835 PREDICTED: similar to CG1532-PA isoform 1 [Bos taurus] 5.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR004360 EMBL-EBI Glyoxalase/Bleomycin resistance protein/dioxygenase domain 9.3E-7
Pfam IPR004360 EMBL-EBI Glyoxalase/Bleomycin resistance protein/dioxygenase domain 4.0E-18
Sequence
Target Sequence 
>C. ELEGANS:189158_AT
cgtcatcgattctgatcaactcttcgaaaaagtcgaaaaaatcaatcatcgtaaatctgg
atgtggaagactagctgtgaaggatccagatggccacgaattcaagatcggaaaggctga
tcaatcgccaaaagtgttgagagttcaagtgaatgttggagacttagagaaatccaagaa
atattggaatgaaactctcggaatgccgattgttgaggaaaagtcgagcagaattcgaat
gagctatggtgatggacagtgtgagctggagattgtgaagtctcaggataaaattgatcg
aaaaactggattcggaagaattgcattcagttatcctgaagataagctcgaatctctcca
agacaagatcaaatcagccaatggaactattataaacgagctcacaactctcgaaactcc
aggaaaagccgatgttcaagttgtgattcttgccgatccagatgagcatgaaatctgttt
tgttggtgatgaaggattcagagctctcagcaaaattgacgataaagccgagtctgagct
caaggagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTCATCGATTCTGATCAACTCTTC 250 247 276 Antisense
GGATCCAGATGGCCACGAATTCAAG 284 513 357 Antisense
TGGAATGAAACTCTCGGAATGCCGA 423 545 460 Antisense
GATAAGCTCGAATCTCTCCAAGACA 387 423 616 Antisense
GGAACTATTATAAACGAGCTCACAA 599 529 658 Antisense
ACAACTCTCGAAACTCCAGGAAAAG 311 113 679 Antisense
AAGCCGATGTTCAAGTTGTGATTCT 607 149 701 Antisense
GTGATTCTTGCCGATCCAGATGAGC 32 485 718 Antisense
GAGCATGAAATCTGTTTTGTTGGTG 364 389 739 Antisense
GATGAAGGATTCAGAGCTCTCAGCA 547 411 763 Antisense
AAAGCCGAGTCTGAGCTCAAGGAGC 266 125 799 Antisense
Sequence Source Affymetrix Proprietary Database