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[189151_s_at] Aminopeptidase

Description T16G12.1 /REP_DB=WormBase Gene ID /WP=CE26001 /TR=Q22531 /GB=CAA82971.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Aminopeptidase
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 32      6grp_highest_nonorm > 52      6grp_total > 22      4grp_total > 61      6grp_highest_sub > 02      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1363
1 2 3 4 range
A 1372.9
(3)
P 		941.2
(1.1)
P 		1028.4
(1.5)
P 		28
(-3)
A 		1345
B 1239
(3)
P 		990.9
(1.8)
P 		1243.4
(3)
P 		24
(-3)
A 		1219
C 1300.5
(3)
P 		753.8
(0.5)
P 		1215.6
(2.6)
P 		10
(-3)
A 		1291
avr 1304.1
(3) 		895.3
(1.1) 		1162.5
(2.3) 		20.7
(-3) 		1283

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 960
1 2 3 4 5 6 range
A 726.7
(0.7)
P 		1054.8
(3)
P 		604.7
(-0.2)
P 		510.3
(-0.8)
P 		394.1
(-1.6)
P 		201.8
(-3)
P 		853
B 717.1
(0.3)
P 		854.7
(1.1)
P 		1154.4
(3)
P 		610.2
(-0.4)
P 		363
(-1.9)
P 		194.6
(-3)
P 		960
C 760.2
(1.1)
P 		873.9
(1.9)
P 		1023.7
(3)
P 		576.6
(-0.3)
P 		377.2
(-1.7)
P 		204.4
(-3)
P 		819
avr 734.7
(1.4) 		927.8
(3) 		927.6
(3) 		565.7
(0) 		378.1
(-1.5) 		200.3
(-3) 		728
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 498
1 2 3 4 range
A 499.9
(3)
P 		204.3
(-0.6)
P 		268.8
(0.2)
P 		4.1
(-3)
A 		496
B 395.1
(3)
P 		244.7
(0.7)
P 		288.1
(1.4)
P 		5.1
(-3)
A 		390
C 430.5
(3)
P 		216.7
(0)
P 		423.7
(2.9)
P 		1.7
(-3)
A 		429
avr 441.8
(3) 		221.9
(-0) 		326.9
(1.4) 		3.6
(-3) 		438

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 326
1 2 3 4 5 6 range
A 134
(-1.4)
P 		375.1
(3)
P 		131.7
(-1.5)
P 		129.4
(-1.5)
P 		118.9
(-1.7)
P 		49.1
(-3)
P 		326
B 249.5
(2)
P 		284.5
(3)
P 		280.3
(2.9)
P 		161.6
(-0.5)
P 		147.5
(-0.9)
P 		72.6
(-3)
P 		212
C 281.8
(3)
P 		266.7
(2.6)
P 		260.6
(2.4)
P 		116
(-1.7)
P 		122
(-1.5)
P 		68.4
(-3)
P 		213
avr 221.8
(0.9) 		308.8
(3) 		224.2
(0.9) 		135.7
(-1.2) 		129.5
(-1.4) 		63.4
(-3) 		245
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189151_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6680
Sequence Type Exemplar sequence
Representative Public ID T16G12.1 NCBI
Target Description T16G12.1 /REP_DB=WormBase Gene ID /WP=CE26001 /TR=Q22531 /GB=CAA82971.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Aminopeptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066829(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066829 NCBI Caenorhabditis elegans T16G12.1 (Aminopeptidase) mRNA, complete cds. 11/11 A 
GENEFINDER00000010177 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10038512:10050731:-1 11/11 None
SNAP00000010162 ENSEMBL cdna:SNAP chromosome:CEL140:III:10040140:10048601:-1 11/11 None
T16G12.1 ENSEMBL cdna:known chromosome:CEL140:III:10040080:10049670:-1 gene:T16G12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10040364-10049628(-) 100.0 100.0
Public Domain and Genome References
Gene Title Aminopeptidase
Ensembl T16G12.1
Entrez Gene 176416 Entrez gene
SwissProt Q22531 EMBL-EBI
WormBase CE36716 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4179 membrane alanyl aminopeptidase activity inferred from electronic annotation QuickGO AmiGO
5507 copper ion binding inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA82971 Hypothetical protein T16G12.1 [Caenorhabditis elegans] ref|NP_499230.2| T16G12.1 [Caenorhabditis elegans] 0.0
blast CAE65056 Hypothetical protein CBG09901 [Caenorhabditis briggsae] 0.0
blast CAA82973 Hypothetical protein T16G12.7 [Caenorhabditis elegans] emb|CAA83616.1| Hypothetical protein T16G12.7 [Caenorhabditis elegans] ref|NP_499229.1| T16G12.7 [Caenorhabditis elegans] 1.0E-171
blast XP_393122 PREDICTED: similar to CG14516-PA, isoform A, partial [Apis mellifera] 1.0E-124
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 4.0E-39
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499230.2 1 5-24
T16G12.1 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:189151_S_AT
ggattcacttgcaccatgggaacaggctatcgagttcttcactgaaatgttaaatagatt
ccaataccaaccagaaattgatactgtaagaaactatgttattcaaattacgaaaaacgc
agtgtccaagttccaatggaacacgaatggcctttgggcaaacgatcgaattgtacaact
cctagtaaacgtcaataacttggctgttaatcgacaatctaggcaggttgctctaacttt
gttcaacaactttgtattgaaatgcaagtactcgctttctggaactggaaagtgctcagg
aattcatccaaatcttcgacaacccacttattgttatggccttcgtcaaagtaataatat
cgatgattttacaacagtgaacaacctatactcgtggtttgtacaaaatgctggctactt
gcaaacagatggatctaatcttttgaatgctctgggatgcgtacaaaatcttgatttgca
aaagacaatgcttcgatcaattctgagtggagattatccaccatcacttctcaattcaat
tgcagttcatgatgattcatcagatgttctttacaatttcttgctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATTCACTTGCACCATGGGAACAG 329 509 4836 Antisense
AACAGGCTATCGAGTTCTTCACTGA 539 133 4856 Antisense
AACGCAGTGTCCAAGTTCCAATGGA 553 145 4951 Antisense
ATGGAACACGAATGGCCTTTGGGCA 356 51 4971 Antisense
GGCAGGTTGCTCTAACTTTGTTCAA 235 535 5057 Antisense
ATGCAAGTACTCGCTTTCTGGAACT 62 33 5097 Antisense
CAAATCTTCGACAACCCACTTATTG 460 193 5144 Antisense
CACTTATTGTTATGGCCTTCGTCAA 40 195 5160 Antisense
AACAGTGAACAACCTATACTCGTGG 412 123 5208 Antisense
GAATGCTCTGGGATGCGTACAAAAT 672 329 5280 Antisense
GATGTTCTTTACAATTTCTTGCTGG 83 411 5398 Antisense
Sequence Source Affymetrix Proprietary Database