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[189147_at] EGF-like domain

Description F58E6.3 /REP_DB=WormBase Gene ID /WP=CE06018 /TR=Q20979 /GB=CAA94773.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=EGF-like domain
Cluster 6grp_total > 28      4grp_total > 02      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 18.5
(-2)
A 		2
(-3)
A 		5.5
(-2.8)
A 		97.1
(3)
P 		95
B 13.6
(-3)
A 		23
(-1.3)
A 		25.6
(-0.8)
A 		46.7
(3)
A 		33
C 21.4
(-2.3)
A 		23.6
(-2.1)
A 		14.7
(-3)
A 		71.6
(3)
A 		57
avr 17.8
(-2.7) 		16.2
(-2.9) 		15.3
(-3) 		71.8
(3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 90.5
(3)
A 		14.9
(-3)
A 		74.2
(1.7)
A 		43.8
(-0.7)
A 		55.2
(0.2)
A 		30
(-1.8)
A 		76
B 54.4
(3)
A 		29.4
(-2.1)
A 		35.9
(-0.8)
A 		24.8
(-3)
A 		48.5
(1.8)
A 		49.1
(1.9)
A 		30
C 40.6
(0.1)
A 		54.3
(1.8)
A 		17
(-2.7)
A 		14.8
(-3)
A 		16.4
(-2.8)
A 		64.5
(3)
A 		50
avr 61.8
(3) 		32.9
(-2.1) 		42.4
(-0.4) 		27.8
(-3) 		40
(-0.8) 		47.9
(0.5) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 6.7
(-0.3)
A 		0.4
(-3)
A 		1.4
(-2.6)
A 		14.2
(3)
P 		14
B 4.3
(-3)
A 		5.7
(-1.5)
A 		5.9
(-1.3)
A 		10
(3)
A 		6
C 7.1
(-1.3)
A 		6.8
(-1.5)
A 		5.1
(-3)
A 		12.1
(3)
A 		7
avr 6
(-1.6) 		4.3
(-2.9) 		4.1
(-3) 		12.1
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 16.7
(3)
A 		5.3
(-3)
A 		16.2
(2.7)
A 		11.1
(0.1)
A 		16.7
(3)
A 		7.3
(-1.9)
A 		11
B 18.9
(2.6)
A 		9.8
(-1.5)
A 		8.7
(-2)
A 		6.6
(-3)
A 		19.7
(3)
A 		18.3
(2.4)
A 		13
C 15.1
(0.9)
A 		16.6
(1.4)
A 		4.3
(-2.6)
A 		3
(-3)
A 		5.3
(-2.3)
A 		21.6
(3)
A 		19
avr 16.9
(3) 		10.6
(-0.8) 		9.7
(-1.3) 		6.9
(-3) 		13.9
(1.2) 		15.7
(2.3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189147_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11309
Sequence Type Exemplar sequence
Representative Public ID F58E6.3 NCBI
Target Description F58E6.3 /REP_DB=WormBase Gene ID /WP=CE06018 /TR=Q20979 /GB=CAA94773.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=EGF-like domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073112(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073112 NCBI Caenorhabditis elegans F58E6.3 (F58E6.3) mRNA, complete cds. 11/11 None
SNAP00000040365 ENSEMBL cdna:SNAP chromosome:CEL140:V:9750648:9751681:1 11/11 None
GENEFINDER00000040379 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9750648:9751681:1 11/11 None
F58E6.3 ENSEMBL cdna:known chromosome:CEL140:V:9750648:9751810:1 gene:F58E6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9750647-9751681(+) 100.0 100.0
Public Domain and Genome References
Gene Title EGF-like domain
Ensembl F58E6.3
Entrez Gene 179367 Entrez gene
SwissProt Q20979 EMBL-EBI
WormBase CE06018 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94773 Hypothetical protein F58E6.3 [Caenorhabditis elegans] ref|NP_505513.1| F58E6.3 [Caenorhabditis elegans] 1.0E-178
blast CAA94773 Hypothetical protein F58E6.3 [Caenorhabditis elegans] ref|NP_505513.1| F58E6.3 [Caenorhabditis elegans] 1.0E-174
blast CAE72246 Hypothetical protein CBG19364 [Caenorhabditis briggsae] 1.0E-164
blast CAE72246 Hypothetical protein CBG19364 [Caenorhabditis briggsae] 1.0E-159
blast CAE61469 Hypothetical protein CBG05362 [Caenorhabditis briggsae] 2.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 7.8E-7
Pfam IPR006209 EMBL-EBI EGF-like domain 7.8E-7
Sequence
Target Sequence 
>C. ELEGANS:189147_AT
gatggttccacgtgtgaaccttgcccaattggatcgtttaaaagtgcggatgacatggtt
tgtatgatgtgcccaactggaagaactactctttcaaaagcttcgaaagcattgtctgca
tgccacattaaaatctgtttccctgggacaattcttgaccacagtactttcaagtgtgaa
ccttgtgattttggaacctatatggatgagtatgatggaagaatctgcaaaacttgtccg
gtatccacaacaacttatcagcagggcgcaaacactgcaaaaatgtgtgaatggacgaac
cagtgcaaagcttctactcataactgtcattggcttgctgcttgtattgatttgcccgat
gagaatcataagaaaatgtactcatgcaagtgcaagccgggatttgttggaaatggattc
cattgtgttgatgcctgtgaaggattttgtttgaatggaggttcttgcttaaaaactgga
agaggagaaacgaaatgcctctgtgcaagtggattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGGTTCCACGTGTGAACCTTGCC 547 405 349 Antisense
GAACCTTGCCCAATTGGATCGTTTA 361 345 364 Antisense
GAAAGCATTGTCTGCATGCCACATT 435 359 453 Antisense
GACAATTCTTGACCACAGTACTTTC 166 365 495 Antisense
TCTGCAAAACTTGTCCGGTATCCAC 689 607 572 Antisense
CACAACAACTTATCAGCAGGGCGCA 686 193 594 Antisense
GAACCAGTGCAAAGCTTCTACTCAT 213 345 645 Antisense
CATAACTGTCATTGGCTTGCTGCTT 542 213 667 Antisense
TTGCTGCTTGTATTGATTTGCCCGA 278 703 683 Antisense
GGATTCCATTGTGTTGATGCCTGTG 396 509 763 Antisense
GAAATGCCTCTGTGCAAGTGGATTT 86 363 840 Antisense
Sequence Source Affymetrix Proprietary Database