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[189144_at] protein kinase

Description Y4C6A.1 /REP_DB=WormBase Gene ID /WP=CE24395 /TR=Q9N4T6 /GB=AAF60740.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 89      4grp_total > 25      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 7.2
(-3)
A 		13.5
(-0.6)
A 		10.3
(-1.8)
A 		23.1
(3)
A 		16
B 3.8
(-3)
A 		23.4
(3)
A 		9.4
(-1.3)
A 		10.1
(-1.1)
A 		20
C 9.9
(-3)
A 		12
(-2.6)
A 		28.8
(0.3)
A 		44.2
(3)
A 		34
avr 7
(-3) 		16.3
(-0) 		16.2
(-0.1) 		25.8
(3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 32
(-1.6)
A 		27.7
(-2)
A 		28.3
(-1.9)
A 		23.3
(-2.4)
A 		17.7
(-3)
A 		77.9
(3)
P 		60
B 7.2
(-2.4)
A 		13.8
(-1.6)
A 		10.9
(-2)
A 		2.6
(-3)
A 		51.1
(3)
A 		24.9
(-0.2)
A 		49
C 3.1
(-3)
A 		11.5
(-1.8)
A 		8.3
(-2.3)
A 		3.4
(-3)
A 		44.7
(3)
A 		18.9
(-0.7)
A 		42
avr 14.1
(-2.2) 		17.7
(-1.5) 		15.8
(-1.8) 		9.8
(-3) 		37.8
(2.5) 		40.6
(3) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 2.6
(-3)
A 		2.9
(-0.8)
A 		2.7
(-2.3)
A 		3.4
(3)
A 		1
B 1.2
(-3)
A 		5.8
(3)
A 		2.2
(-1.7)
A 		2.1
(-1.8)
A 		5
C 3.3
(-3)
A 		3.5
(-2.8)
A 		10
(3)
A 		7.5
(0.8)
A 		7
avr 2.4
(-3) 		4.1
(0.9) 		5
(3) 		4.3
(1.4) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 5.9
(-2.8)
A 		9.9
(-1)
A 		6.2
(-2.6)
A 		5.9
(-2.8)
A 		5.4
(-3)
A 		19
(3)
P 		14
B 2.5
(-2.5)
A 		4.6
(-1.8)
A 		2.6
(-2.4)
A 		0.7
(-3)
A 		20.7
(3)
A 		9.3
(-0.4)
A 		20
C 1.1
(-2.8)
A 		3.5
(-1.8)
A 		2.1
(-2.4)
A 		0.7
(-3)
A 		14.5
(3)
A 		6.3
(-0.6)
A 		14
avr 3.2
(-2.6) 		6
(-1.1) 		3.6
(-2.4) 		2.4
(-3) 		13.5
(3) 		11.5
(1.9) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189144_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10561
Sequence Type Exemplar sequence
Representative Public ID Y4C6A.1 NCBI
Target Description Y4C6A.1 /REP_DB=WormBase Gene ID /WP=CE24395 /TR=Q9N4T6 /GB=AAF60740.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068411
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
Y4C6A.1 ENSEMBL cdna:known chromosome:CEL140:IV:5458868:5460722:1 gene:Y4C6A.1 None
SNAP00000043997 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5458868:5460722:1 None
NM_068411 NCBI Caenorhabditis elegans Y4C6A.1 (Y4C6A.1) mRNA, complete cds. None
GENEFINDER00000044000 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5458868:5460722:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5458873-5461013(+) 88.19 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl Y4C6A.1
Entrez Gene 189353 Entrez gene
SwissProt Q9N4T6 EMBL-EBI
WormBase CE36748 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60740 Hypothetical protein Y4C6A.1 [Caenorhabditis elegans] ref|NP_500812.2| Y4C6A.1 [Caenorhabditis elegans] 0.0
blast NP_501307 T06C10.3 [Caenorhabditis elegans] gb|AAA82300.1| Hypothetical protein T06C10.3 [Caenorhabditis elegans] 1.0E-152
blast CAB02882 Hypothetical protein F01D4.3 [Caenorhabditis elegans] ref|NP_501934.2| F01D4.3 [Caenorhabditis elegans] 1.0E-145
blast NP_501307 T06C10.3 [Caenorhabditis elegans] gb|AAA82300.1| Hypothetical protein T06C10.3 [Caenorhabditis elegans] 1.0E-145
blast NP_501307 T06C10.3 [Caenorhabditis elegans] gb|AAA82300.1| Hypothetical protein T06C10.3 [Caenorhabditis elegans] 1.0E-142
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 6.7E-5
Pfam IPR000980 EMBL-EBI SH2 motif 0.13
Pfam IPR000719 EMBL-EBI Protein kinase 1.7E-37
Pfam IPR000719 EMBL-EBI Protein kinase 6.3E-28
Pfam IPR000719 EMBL-EBI Protein kinase 3.5E-31
Sequence
Target Sequence 
>C. ELEGANS:189144_AT
gtaactgtcttctccaaacgagttgggggatcacgtgctacgaagtgttcgccgatggag
gtgagccgtttgaaggtatgccgaatgcagttgtaaattcggatgtactcgcgaacaagt
tctgttcaatgccaccaaattgtccggaaaccatcaaaacctacctgaacacgttcgttt
ttgtcgaggaaccccgccgcgcgacgatgacactggctgtgacggagtttgaacgatttg
cggtaatgtgtgagactggacagatggaaatgccaaagaagaagattgcggatgttttca
agagtcatagaagtagccgaagtaagagaaagaagaaggagaaggaggcacccaagacag
atttatgttttttcgctcaagattacagaaaacagtttcaatacaaatatacatggtttg
atgtaccataccaggattcagagtctccccaagaatgtttatcctcaaaaataatgcgag
cgcgcttcaacaaacgagtta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAACTGTCTTCTCCAAACGAGTTG 75 445 995 Antisense
GATCACGTGCTACGAAGTGTTCGCC 236 421 1023 Antisense
AGTGTTCGCCGATGGAGGTGAGCCG 357 59 1038 Antisense
GGTGAGCCGTTTGAAGGTATGCCGA 470 499 1054 Antisense
AACAAGTTCTGTTCAATGCCACCAA 339 135 1108 Antisense
GCCACCAAATTGTCCGGAAACCATC 201 279 1125 Antisense
TCAAAACCTACCTGAACACGTTCGT 492 627 1148 Antisense
GCGACGATGACACTGGCTGTGACGG 254 289 1195 Antisense
CATACCAGGATTCAGAGTCTCCCCA 8 213 1421 Antisense
GTCTCCCCAAGAATGTTTATCCTCA 193 471 1437 Antisense
CGAGCGCGCTTCAACAAACGAGTTA 365 249 1471 Antisense
Sequence Source Affymetrix Proprietary Database