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[189143_at] 7TM chemoreceptor, srg family

Description Y25C1A.9 /REP_DB=WormBase Gene ID /WP=CE21477 /TR=Q9TYL8 /GB=AAD12840.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 19      4grp_total > 40      6grp_cgc > 80      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 9
(-3)
A 		19.7
(-1.9)
A 		26.9
(-1.2)
A 		68.7
(3)
A 		60
B 43.5
(-0.6)
A 		36.9
(-1.8)
A 		64.5
(3)
A 		29.9
(-3)
A 		35
C 40.1
(2.7)
A 		36.8
(2.2)
A 		42.1
(3)
A 		1.7
(-3)
A 		40
avr 30.9
(-3) 		31.1
(-2.9) 		44.5
(3) 		33.4
(-1.9) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 64.8
(3)
A 		21.9
(-1.1)
A 		40.6
(0.7)
A 		2.5
(-3)
A 		26
(-0.7)
A 		4.2
(-2.8)
A 		62
B 25.9
(3)
A 		22.6
(2.2)
A 		20.2
(1.6)
A 		21.2
(1.8)
A 		22.9
(2.3)
A 		1.5
(-3)
A 		24
C 2.8
(-3)
A 		6.2
(-2.1)
A 		8.7
(-1.4)
A 		6.3
(-2.1)
A 		25.2
(3)
A 		13.2
(-0.2)
A 		22
avr 31.2
(3) 		16.9
(-0.4) 		23.2
(1.1) 		10
(-2.1) 		24.7
(1.4) 		6.3
(-3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 3.3
(-3)
A 		4.3
(-2.1)
A 		7
(0.3)
A 		10.1
(3)
A 		7
B 13.9
(2.3)
A 		9.1
(-1.1)
A 		14.9
(3)
A 		6.4
(-3)
A 		9
C 13.3
(2.4)
A 		10.6
(1.3)
A 		14.7
(3)
A 		0.3
(-3)
A 		14
avr 10.2
(1.2) 		8
(-0.8) 		12.2
(3) 		5.6
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 11.9
(3)
A 		7.8
(0.8)
A 		8.8
(1.4)
A 		0.6
(-3)
A 		7.9
(0.9)
A 		1
(-2.8)
A 		11
B 9
(2.8)
A 		7.5
(1.8)
A 		4.9
(-0)
A 		5.6
(0.4)
A 		9.3
(3)
A 		0.6
(-3)
A 		9
C 1
(-3)
A 		1.9
(-2.2)
A 		2.2
(-2)
A 		1.3
(-2.7)
A 		8.1
(3)
A 		4.4
(-0.1)
A 		7
avr 7.3
(2) 		5.7
(0.5) 		5.3
(0.1) 		2.5
(-2.5) 		8.4
(3) 		2
(-3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189143_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5961
Sequence Type Exemplar sequence
Representative Public ID Y25C1A.9 NCBI
Target Description Y25C1A.9 /REP_DB=WormBase Gene ID /WP=CE21477 /TR=Q9TYL8 /GB=AAD12840.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062041(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062041 NCBI Caenorhabditis elegans Serpentine Receptor, class G (gamma) family member (srg-21) (srg-21) mRNA, complete cds. 11/11 None
Y25C1A.9 ENSEMBL cdna:known chromosome:CEL140:II:3088469:3091291:-1 gene:Y25C1A.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041879 7/11 Cross Hyb Matching Probes None
SNAP00000041880 4/11 Cross Hyb Matching Probes None
GENEFINDER00000041894 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3088467-3091290(-) 88.86 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srg family
Gene Symbol srg-21
Ensembl Y25C1A.9
Entrez Gene 189538 Entrez gene
SwissProt Q9TYL8 EMBL-EBI
WormBase CE33211 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494442 Serpentine Receptor, class G (gamma) family member (srg-21) [Caenorhabditis elegans] gb|AAD12840.2| Serpentine receptor, class g (gamma) protein 21 [Caenorhabditis elegans] 1.0E-175
blast NP_494445 Serpentine Receptor, class G (gamma) family member (srg-24) [Caenorhabditis elegans] gb|AAD12842.2| Serpentine receptor, class g (gamma) protein 24 [Caenorhabditis elegans] 1.0E-132
blast NP_494443 Serpentine Receptor, class G (gamma) family member (srg-22) [Caenorhabditis elegans] gb|AAD12845.2| Serpentine receptor, class g (gamma) protein 22 [Caenorhabditis elegans] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494442.2 7 32-54,67-89,104-126,159-181,201-223,243-265,275-297
Y25C1A.9 7 32-54,67-89,104-126,159-181,201-223,243-265,275-297
Sequence
Target Sequence 
>C. ELEGANS:189143_AT
gctggcattcaagactttatcgcaaattgcagtcagcttcaatagaatgacatgtgttat
tcttccagttcggcacgtacatttatggaaaaggatactatggccaatggtgattgctca
atttgtgctccctctcggagtaatctggaatatcctactatctagggtgtatattaatcc
aaacggtgctgggttttccgtcaactacaaggatgcgattccatgggccaacatctcctt
cctacacctattccactgtatcccatgtgttgtattagttaccatattctttatagtcac
catatatggcctaacaatactggaatatagaatcaaaaacgttgagagatacctgacaat
tttcactttaataatgggtattcagacggcgttgttcgcttttacgcagatctactttgc
cttcttcactgcctaccttccaggcatccgaagtttcatgttattctccgcatttcatat
atttgatctactccatgtctacagcccaattgcacttattat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGCATTCAAGACTTTATCGCAA 265 295 594 Antisense
TATCGCAAATTGCAGTCAGCTTCAA 1 659 611 Antisense
TATTCTTCCAGTTCGGCACGTACAT 270 659 651 Antisense
TGCTCCCTCTCGGAGTAATCTGGAA 694 581 719 Antisense
GGGTATTCAGACGGCGTTGTTCGCT 399 497 969 Antisense
GTTGTTCGCTTTTACGCAGATCTAC 92 439 984 Antisense
CTACCTTCCAGGCATCCGAAGTTTC 34 215 1026 Antisense
GAAGTTTCATGTTATTCTCCGCATT 439 337 1043 Antisense
TATTCTCCGCATTTCATATATTTGA 109 665 1055 Antisense
ATTTGATCTACTCCATGTCTACAGC 39 19 1074 Antisense
TCTACAGCCCAATTGCACTTATTAT 88 615 1091 Antisense
Sequence Source Affymetrix Proprietary Database