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[189142_at] cytochrome P450

Description B0331.1 /REP_DB=WormBase Gene ID /WP=CE15551 /TR=O45219 /GB=CAB11775.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 90      4grp_total > 29      6grp_cgc > 96      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 56.1
(3)
A 		27.7
(-1.7)
A 		20.9
(-2.9)
A 		20
(-3)
A 		36
B 52.2
(2.7)
A 		54.5
(3)
A 		6.9
(-3)
A 		30
(-0.1)
A 		48
C 55.1
(-1.4)
A 		61.7
(-0.9)
A 		30.8
(-3)
A 		121
(3)
A 		90
avr 54.5
(2.6) 		48
(1.6) 		19.5
(-3) 		57
(3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 103
(3)
A 		25.8
(-2.1)
A 		11.3
(-3)
A 		51.7
(-0.4)
A 		69.8
(0.8)
A 		33.3
(-1.6)
A 		92
B 64
(0.1)
A 		38.9
(-2.7)
A 		35.8
(-3)
A 		90
(3)
A 		50.1
(-1.4)
A 		60.5
(-0.3)
A 		54
C 9.9
(-3)
A 		27.6
(-2)
A 		61.9
(-0.1)
A 		117.4
(3)
A 		59.2
(-0.2)
A 		51
(-0.7)
A 		108
avr 59
(0) 		30.8
(-3) 		36.3
(-2.4) 		86.4
(3) 		59.7
(0.1) 		48.3
(-1.1) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 20.4
(3)
A 		6
(-1.9)
A 		5.5
(-2.1)
A 		2.9
(-3)
A 		18
B 16.6
(3)
A 		13.5
(1.8)
A 		1.6
(-3)
A 		6.4
(-1.1)
A 		15
C 18.2
(1.6)
A 		17.7
(1.3)
A 		10.7
(-3)
A 		20.4
(3)
A 		10
avr 18.4
(3) 		12.4
(0.1) 		5.9
(-3) 		9.9
(-1.1) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 19
(2.3)
A 		9.2
(-0.8)
A 		2.5
(-3)
A 		13.1
(0.4)
A 		21.1
(3)
A 		8.1
(-1.2)
A 		19
B 22.3
(2.4)
A 		12.9
(-1.3)
A 		8.7
(-3)
A 		23.8
(3)
A 		20.4
(1.6)
A 		22.6
(2.5)
A 		15
C 3.7
(-3)
A 		8.4
(-1.6)
A 		15.7
(0.6)
A 		23.6
(3)
A 		19.1
(1.6)
A 		17.1
(1)
A 		20
avr 15
(0.2) 		10.2
(-2.4) 		9
(-3) 		20.2
(3) 		20.2
(3) 		15.9
(0.7) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189142_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12372
Sequence Type Exemplar sequence
Representative Public ID B0331.1 NCBI
Target Description B0331.1 /REP_DB=WormBase Gene ID /WP=CE15551 /TR=O45219 /GB=CAB11775.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073089(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073089 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-29A4) (cyp-29A4) mRNA, complete cds. 11/11 None
SNAP00000018467 ENSEMBL cdna:SNAP chromosome:CEL140:V:9658414:9660479:1 11/11 None
GENEFINDER00000018469 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9658414:9660970:1 11/11 None
B0331.1 ENSEMBL cdna:known chromosome:CEL140:V:9657341:9660479:1 gene:B0331.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9657340-9660479(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-29A4
Ensembl B0331.1
Entrez Gene 181926 Entrez gene
SwissProt O45219 EMBL-EBI
WormBase CE15551 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1636793_AT Cytochrome P450-4d2 dm
DROSGENOME1:152267_AT Cytochrome P450-4d2 dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB11775 Hypothetical protein B0331.1 [Caenorhabditis elegans] ref|NP_505490.1| CYtochrome P450 family member (cyp-29A4) [Caenorhabditis elegans] 0.0
blast CAE72221 Hypothetical protein CBG19332 [Caenorhabditis briggsae] 0.0
blast CAE75191 Hypothetical protein CBG23137 [Caenorhabditis briggsae] 1.0E-175
Protein Families
Method ID Description E-Value
p450 CP15_CHICK Animalia; CYP1A5; chi; CP15_CHICK; Gallus gallus; P79761 1.0E-107
p450 CP15_CHICK Animalia; CYP1A5; chi; CP15_CHICK; Gallus gallus; P79761 1.0E-108
p450 CP11_ONCMY Animalia; CYP1A2; tro; CP11_ONCMY; Trout; Q92110 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.3E-104
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.3E-104
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505490.1 1 2-19
B0331.1 1 2-19
Sequence
Target Sequence 
>C. ELEGANS:189142_AT
gatacaacttcaacatctctttcatggctttgttggaatattgcacataatccagaagtt
caagaaaatgtttacaaagagattattagcatttttggagaagatccaaatcaagatgta
acttctgaaaatatcaaccgattggagtatacggaacgagttttgaaagaatcaaaaaga
atgtttccaccagttccaggatttcaaagaaaattaacaaaggacatagttattgatgga
ataacaataccctcagagggtaacatcactatctccccaacagttcttcactgcaatccg
tttgtataccaaaatcctgaaaaatttgatccagatcggtttcttcctgaagagtgcgca
aaacgacattcctacgattatataccattctccgctggacttcgaaactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACAACTTCAACATCTCTTTCAT 545 423 946 Antisense
ACATCTCTTTCATGGCTTTGTTGGA 123 107 958 Antisense
GAATGTTTCCACCAGTTCCAGGATT 32 331 1124 Antisense
GATGGAATAACAATACCCTCAGAGG 675 405 1180 Antisense
GAGGGTAACATCACTATCTCCCCAA 115 401 1201 Antisense
TCCCCAACAGTTCTTCACTGCAATC 538 595 1219 Antisense
TTCACTGCAATCCGTTTGTATACCA 82 685 1232 Antisense
TGATCCAGATCGGTTTCTTCCTGAA 709 565 1272 Antisense
GAGTGCGCAAAACGACATTCCTACG 489 401 1297 Antisense
CCTACGATTATATACCATTCTCCGC 661 265 1316 Antisense
CATTCTCCGCTGGACTTCGAAACTG 346 217 1331 Antisense
Sequence Source Affymetrix Proprietary Database