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[189139_at] EGF-like domain

Description F25D7.5 /REP_DB=WormBase Gene ID /WP=CE09633 /TR=Q93563 /GB=CAB01698.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=EGF-like domain
Cluster 6grp_total > 74      4grp_total > 48      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 2
(-3)
A 		3.6
(0.8)
A 		4.3
(2.5)
A 		4.5
(3)
A 		3
B 5.6
(-2.2)
A 		5.6
(-2.2)
A 		22.8
(3)
A 		3.1
(-3)
A 		20
C 6.1
(-0.2)
A 		9.7
(3)
A 		3
(-3)
A 		8.7
(2.1)
A 		7
avr 4.6
(-3) 		6.3
(-1.1) 		10
(3) 		5.4
(-2.1) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 7.3
(-1.5)
A 		3
(-3)
A 		4.4
(-2.5)
A 		6
(-2)
A 		20.5
(3)
A 		4.2
(-2.6)
A 		18
B 3.6
(-2.8)
A 		4
(-2.7)
A 		29.3
(3)
A 		3.2
(-2.9)
A 		2.7
(-3)
A 		5.3
(-2.4)
A 		27
C 3.7
(-2.9)
A 		3.4
(-3)
A 		7.5
(-1.9)
A 		25.9
(3)
A 		3.8
(-2.9)
A 		19.7
(1.3)
A 		23
avr 4.9
(-2.2) 		3.5
(-3) 		13.7
(3) 		11.7
(1.8) 		9
(0.2) 		9.7
(0.6) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 0.7
(-3)
A 		0.8
(-1.5)
A 		1.1
(3)
A 		0.7
(-3)
A 		0
B 1.8
(-1.6)
A 		1.4
(-2.1)
A 		5.3
(3)
A 		0.7
(-3)
A 		5
C 2
(0.3)
A 		2.8
(3)
A 		1
(-3)
A 		1.5
(-1.3)
A 		2
avr 1.5
(-1) 		1.7
(-0.2) 		2.5
(3) 		1
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 1.3
(-2.7)
A 		1.1
(-2.9)
A 		1
(-3)
A 		1.5
(-2.4)
A 		6.2
(3)
A 		1
(-3)
A 		5
B 1.2
(-2.7)
A 		1.3
(-2.6)
A 		7.1
(3)
A 		0.9
(-3)
A 		1.1
(-2.8)
A 		2
(-1.9)
A 		6
C 1.4
(-2.6)
A 		1
(-3)
A 		1.9
(-2)
A 		5.2
(1.5)
A 		1.2
(-2.8)
A 		6.6
(3)
A 		6
avr 1.3
(-2.5) 		1.1
(-3) 		3.3
(3) 		2.5
(0.8) 		2.8
(1.6) 		3.2
(2.7) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189139_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1925
Sequence Type Exemplar sequence
Representative Public ID F25D7.5 NCBI
Target Description F25D7.5 /REP_DB=WormBase Gene ID /WP=CE09633 /TR=Q93563 /GB=CAB01698.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=EGF-like domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060324(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060324 NCBI Caenorhabditis elegans F25D7.5 (F25D7.5) mRNA, complete cds. 11/11 None
SNAP00000011702 ENSEMBL cdna:SNAP chromosome:CEL140:I:10431142:10435095:-1 10/11 None
F25D7.5 ENSEMBL cdna:known chromosome:CEL140:I:10431309:10435224:-1 gene:F25D7.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10431239-10435155(-) 100.0 100.0
Public Domain and Genome References
Gene Title EGF-like domain
Ensembl F25D7.5
Entrez Gene 184923 Entrez gene
SwissProt Q93563 EMBL-EBI
WormBase CE09633 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01698 Hypothetical protein F25D7.5 [Caenorhabditis elegans] ref|NP_492725.1| F25D7.5 [Caenorhabditis elegans] 0.0
blast CAE58937 Hypothetical protein CBG02205 [Caenorhabditis briggsae] 0.0
blast CAA21752 Hypothetical protein Y70G10A.2 [Caenorhabditis elegans] ref|NP_499266.1| Y70G10A.2 [Caenorhabditis elegans] 1.0E-129
blast CAA21752 Hypothetical protein Y70G10A.2 [Caenorhabditis elegans] ref|NP_499266.1| Y70G10A.2 [Caenorhabditis elegans] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 9.1E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 9.1E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492725.1 3 361-380,443-460,470-492
F25D7.5 3 361-380,443-460,470-492
SNAP00000011702 5 255-273,301-323,344-366,405-427,434-456
Sequence
Target Sequence 
>C. ELEGANS:189139_AT
aaaaagtttcctgctcgatttgctccatcctatgatccgatttccatgtggactcgtgag
gaagtgaaagaaattcaaaagcttccaaaagaacaacaagaaataatgaaacaacaaatg
cttccattgaataaaagattattcctacatcacaaatgggatttgattctcaatcagaaa
ttggaagcaggactggatgtctctgctggtttactggaaattctgcgaactgagataaag
aagcatcatgatttggctggaactcaatttcaaaaagaacaactccaagaaacatttgcg
gaatttgtgaaatcagtcgctgaacgaccaccaagaaatgatttattgggaaccaatgca
aagagtgaagttgtgactctatgtgcagaaaaagaggatggaactgcaagagtgaccaaa
tttatgctagtccccggaatcgatgttttcaaaccagaagagtactatgatgatgctgat
gatattgacagaaagagatttaataatgaaattgataaggtattatattttcgtagcctg
aagggtatttcctttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAAGTTTCCTGCTCGATTTGCTC 687 129 1531 Antisense
TCCATCCTATGATCCGATTTCCATG 602 589 1554 Antisense
GATCCGATTTCCATGTGGACTCGTG 296 417 1564 Antisense
CAGGACTGGATGTCTCTGCTGGTTT 472 209 1718 Antisense
GATGTCTCTGCTGGTTTACTGGAAA 541 409 1726 Antisense
GCATCATGATTTGGCTGGAACTCAA 341 309 1773 Antisense
ATCAGTCGCTGAACGACCACCAAGA 447 29 1842 Antisense
GTGAAGTTGTGACTCTATGTGCAGA 702 481 1895 Antisense
GTGACCAAATTTATGCTAGTCCCCG 130 483 1942 Antisense
GCTAGTCCCCGGAATCGATGTTTTC 225 289 1956 Antisense
GTAGCCTGAAGGGTATTTCCTTTGA 387 457 2063 Antisense
Sequence Source Affymetrix Proprietary Database