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[189127_at] EGF-like domain

Description Y69H2.2 /REP_DB=WormBase Gene ID /WP=CE22820 /TR=Q9XTS9 /GB=CAB11570.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=EGF-like domain
Cluster 6grp_total > 73      4grp_total > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 23.5
(0.6)
A 		15
(-1)
A 		4.5
(-3)
A 		36.1
(3)
A 		32
B 16.3
(-2.6)
A 		15.4
(-3)
A 		27.8
(3)
A 		22.7
(0.5)
A 		12
C 12.5
(-2.1)
A 		10.6
(-2.3)
A 		2.9
(-3)
A 		64.8
(3)
A 		62
avr 17.4
(-1.8) 		13.7
(-2.6) 		11.7
(-3) 		41.2
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 4.9
(-3)
A 		38
(1.2)
P 		13.3
(-1.9)
A 		52.6
(3)
P 		9.1
(-2.5)
A 		15
(-1.7)
A 		48
B 13
(-2.8)
A 		12.1
(-3)
A 		46.3
(3)
P 		21.7
(-1.3)
A 		16.6
(-2.2)
A 		14.2
(-2.6)
A 		34
C 2.2
(-3)
A 		14.1
(-0.5)
A 		30.8
(3)
A 		16.5
(0)
A 		22.8
(1.3)
A 		27.6
(2.3)
P 		29
avr 6.7
(-3) 		21.4
(0.7) 		30.1
(2.9) 		30.3
(3) 		16.2
(-0.6) 		18.9
(0.1) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 8.6
(3)
A 		3.3
(-1.3)
A 		1.2
(-3)
A 		5.3
(0.3)
A 		7
B 5.2
(0.2)
A 		3.8
(-3)
A 		6.4
(3)
A 		4.8
(-0.7)
A 		3
C 4.1
(-1.1)
A 		3.1
(-1.7)
A 		1
(-3)
A 		10.9
(3)
A 		10
avr 6
(1.5) 		3.4
(-2.3) 		2.9
(-3) 		7
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 0.9
(-3)
A 		13.5
(3)
P 		2.9
(-2)
A 		13.4
(3)
P 		2.7
(-2.1)
A 		3.6
(-1.7)
A 		13
B 4.5
(-2.6)
A 		4
(-3)
A 		11.3
(3)
P 		5.8
(-1.5)
A 		6.7
(-0.8)
A 		5.3
(-1.9)
A 		7
C 0.8
(-3)
A 		4.3
(-0.5)
A 		7.8
(1.9)
A 		3.3
(-1.2)
A 		7.4
(1.7)
A 		9.3
(3)
P 		9
avr 2.1
(-3) 		7.3
(2.8) 		7.3
(2.8) 		7.5
(3) 		5.6
(0.9) 		6.1
(1.4) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189127_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13978
Sequence Type Exemplar sequence
Representative Public ID Y69H2.2 NCBI
Target Description Y69H2.2 /REP_DB=WormBase Gene ID /WP=CE22820 /TR=Q9XTS9 /GB=CAB11570.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=EGF-like domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075237(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075237 NCBI Caenorhabditis elegans Y69H2.2 (Y69H2.2) mRNA, complete cds. 11/11 None
SNAP00000002595 ENSEMBL cdna:SNAP chromosome:CEL140:V:18625807:18648696:-1 9/11 None
GENEFINDER00000002615 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18622787:18637063:-1 11/11 None
Y69H2.2 ENSEMBL cdna:known chromosome:CEL140:V:18631254:18638581:-1 gene:Y69H2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18631253-18638581(-) 100.0 100.0
Public Domain and Genome References
Gene Title EGF-like domain
Ensembl Y69H2.2
Entrez Gene 190557 Entrez gene
SwissProt Q9XTS9 EMBL-EBI
WormBase CE22820 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB11570 Hypothetical protein Y69H2.2 [Caenorhabditis elegans] ref|NP_507638.1| Y69H2.2 [Caenorhabditis elegans] 0.0
blast CAB63408 Hypothetical protein Y69H2.11 [Caenorhabditis elegans] ref|NP_507639.1| Y69H2.11 [Caenorhabditis elegans] 0.0
blast CAB63409 Hypothetical protein Y69H2.12 [Caenorhabditis elegans] ref|NP_507640.1| Y69H2.12 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 1.3E-10
Pfam IPR006209 EMBL-EBI EGF-like domain 2.4E-4
Pfam IPR006209 EMBL-EBI EGF-like domain 5.1E-8
Pfam IPR006209 EMBL-EBI EGF-like domain 5.3E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 1.4E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 3.7E-5
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.2E-43
Pfam IPR006209 EMBL-EBI EGF-like domain 1.3E-10
Pfam IPR006209 EMBL-EBI EGF-like domain 2.4E-4
Pfam IPR006209 EMBL-EBI EGF-like domain 5.1E-8
Pfam IPR006209 EMBL-EBI EGF-like domain 5.3E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 1.4E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 3.7E-5
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.5E-29
Pfam IPR006209 EMBL-EBI EGF-like domain 2.4E-4
Pfam IPR006209 EMBL-EBI EGF-like domain 5.1E-8
Pfam IPR006209 EMBL-EBI EGF-like domain 5.3E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 1.4E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 3.7E-5
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 4.1E-40
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507638.1 1 876-898
Y69H2.2 1 876-898
GENEFINDER00000002615 8 1326-1348,1360-1382,1408-1430,1437-1454,1474-1496,1524-1541,1556-1578,1591-1613
Sequence
Target Sequence 
>C. ELEGANS:189127_AT
caagaactactactacccaggagcttataccactgatggctgcctccgcacgtgctacca
agaccgtatgaaagaggagtgcaactgcatggaccccagatacccccaggctccaaattc
aacgtcatgccagttatccgagcggagctgcgtcacagaagcgtcagaagccgccggaga
cccatccacctggtcatcttgtgtatgcccgctgccatgctcgaatcaagagtactcggt
gacttggagcaaggcgaattttgtgaatttgccaataacgtgtgaaaagtcttctgacgt
ggcaacatgccaaaaacagtacaaagatcagttgatggtgtcgataatccttccgcaatt
ggattttaagatctacgcggagacccctgcaatggatttcaacaagtttttgtcacaact
cggaggtcagctaggcgtcctgatgggaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGAACTACTACTACCCAGGAGCT 286 183 2208 Antisense
CTACTACCCAGGAGCTTATACCACT 114 225 2217 Antisense
GTTATCCGAGCGGAGCTGCGTCACA 466 447 2340 Antisense
GGAGCTGCGTCACAGAAGCGTCAGA 446 521 2351 Antisense
AACGTGTGAAAAGTCTTCTGACGTG 10 145 2484 Antisense
GGCAACATGCCAAAAACAGTACAAA 89 533 2508 Antisense
TGATGGTGTCGATAATCCTTCCGCA 220 567 2540 Antisense
TGTCGATAATCCTTCCGCAATTGGA 608 555 2546 Antisense
GCAATTGGATTTTAAGATCTACGCG 628 321 2562 Antisense
GTTTTTGTCACAACTCGGAGGTCAG 245 449 2613 Antisense
GTCAGCTAGGCGTCCTGATGGGAAT 585 463 2633 Antisense
Sequence Source Affymetrix Proprietary Database