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[189126_s_at]

Description C05E4.14 /REP_DB=WormBase Gene ID /WP=CE24788 /GEN=srh-2 /GB=AAK72080.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 66      4grp_total > 46      6grp_cgc > 48      6grp_cgc_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 34.6
(1.3)
A 		46.3
(3)
A 		39.5
(2)
A 		3.9
(-3)
A 		42
B 41.2
(3)
A 		11
(-1.8)
A 		3.3
(-3)
A 		24.8
(0.4)
A 		38
C 70
(3)
A 		10.2
(-2.7)
A 		6.6
(-3)
A 		16.5
(-2.1)
A 		63
avr 48.6
(3) 		22.5
(-1.7) 		16.5
(-2.7) 		15.1
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 277
1 2 3 4 5 6 range
A 19.7
(-3)
A 		45
(-2.5)
A 		41.4
(-2.5)
A 		150.1
(-0.2)
P 		28.4
(-2.8)
A 		296.5
(3)
P 		277
B 49
(-3)
A 		73.5
(-2.1)
A 		76.6
(-2)
A 		68.6
(-2.3)
A 		79
(-2)
A 		221.6
(3)
P 		173
C 84
(-1.8)
A 		69.1
(-2.2)
M 		68.7
(-2.2)
A 		122.5
(-0.8)
A 		37
(-3)
A 		270.7
(3)
P 		234
avr 50.9
(-2.9) 		62.5
(-2.6) 		62.2
(-2.6) 		113.7
(-1.2) 		48.1
(-3) 		262.9
(3) 		215
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 12.6
(3)
A 		10
(1.7)
A 		10.3
(1.9)
A 		0.6
(-3)
A 		12
B 13.2
(3)
A 		2.7
(-2.1)
A 		0.8
(-3)
A 		5.3
(-0.8)
A 		12
C 23.2
(3)
A 		2.9
(-2.8)
A 		2.3
(-3)
A 		2.8
(-2.9)
A 		21
avr 16.3
(3) 		5.2
(-2) 		4.5
(-2.3) 		2.9
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 3.6
(-3)
A 		16
(-1.9)
A 		9
(-2.5)
A 		38.1
(0)
P 		8.6
(-2.6)
A 		72.2
(3)
P 		69
B 17
(-3)
A 		24.5
(-2.3)
A 		18.6
(-2.9)
A 		18.2
(-2.9)
A 		32.1
(-1.6)
A 		82.6
(3)
P 		66
C 31.1
(-1.5)
A 		21.1
(-2.3)
M 		17.5
(-2.6)
A 		24.6
(-2)
A 		12
(-3)
A 		90.6
(3)
P 		79
avr 17.2
(-2.8) 		20.5
(-2.5) 		15
(-3) 		27
(-1.9) 		17.6
(-2.8) 		81.8
(3) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189126_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15681
Sequence Type Exemplar sequence
Representative Public ID C05E4.14 NCBI
Target Description C05E4.14 /REP_DB=WormBase Gene ID /WP=CE24788 /GEN=srh-2 /GB=AAK72080.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026193(11), NM_001026194(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026193 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-2) (srh-2) mRNA, complete cds. 11/11 None
NM_001026194 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-2) (srh-2) mRNA, complete cds. 11/11 None
C05E4.14a ENSEMBL cdna:known chromosome:CEL140:V:731476:734109:1 gene:C05E4.14 11/11 None
C05E4.14b ENSEMBL cdna:known chromosome:CEL140:V:732376:734109:1 gene:C05E4.14 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000009756 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:732375-734109(+) 100.0 100.0
Public Domain and Genome References
Ensembl C05E4.14
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAW88411 Serpentine receptor, class h protein 2, isoform b [Caenorhabditis elegans] ref|NP_001021365.1| Serpentine Receptor, class H family member (srh-2) [Caenorhabditis elegans] 1.0E-171
blast AAK72080 Serpentine receptor, class h protein 2, isoform a [Caenorhabditis elegans] ref|NP_001021364.1| Serpentine Receptor, class H family member (srh-2) [Caenorhabditis elegans] 2.0E-94
blast AAW88411 Serpentine receptor, class h protein 2, isoform b [Caenorhabditis elegans] ref|NP_001021365.1| Serpentine Receptor, class H family member (srh-2) [Caenorhabditis elegans] 4.0E-70
blast AAK72080 Serpentine receptor, class h protein 2, isoform a [Caenorhabditis elegans] ref|NP_001021364.1| Serpentine Receptor, class H family member (srh-2) [Caenorhabditis elegans] 1.0E-69
blast AAF39926 Serpentine receptor, class h protein 7 [Caenorhabditis elegans] ref|NP_504118.1| Serpentine Receptor, class H family member (srh-7) [Caenorhabditis elegans] 5.0E-54
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021364.1 3 64-86,107-129,152-174
NP_001021365.1 7 15-37,50-69,93-115,136-155,194-216,237-259,282-304
C05E4.14a 3 64-86,107-129,152-174
C05E4.14b 7 15-37,50-69,93-115,136-155,194-216,237-259,282-304
Sequence
Target Sequence 
>C. ELEGANS:189126_S_AT
acataatattgtttagctccgggattgtgacctacaaagctttgaaagtgagccgagcat
ctgcagccgttagaaaaattcagaagaacattttcatgagcctggtggtgtcgacaatta
ctcaccttggcctactatttataccactggctacatttttcatgctctccctacttgtgc
ccgaatggagcaacactagtgattttatggtgattatgattcaacaacacggcgcagttt
ccgcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATAATATTGTTTAGCTCCGGGAT 647 105 632 Antisense
TTAGCTCCGGGATTGTGACCTACAA 527 677 644 Antisense
TGAAAGTGAGCCGAGCATCTGCAGC 92 573 674 Antisense
GAAGAACATTTTCATGAGCCTGGTG 509 339 714 Antisense
ATGAGCCTGGTGGTGTCGACAATTA 94 45 727 Antisense
GTGTCGACAATTACTCACCTTGGCC 625 485 739 Antisense
TTACTCACCTTGGCCTACTATTTAT 587 681 749 Antisense
CCTACTATTTATACCACTGGCTACA 670 267 762 Antisense
CACTGGCTACATTTTTCATGCTCTC 352 199 776 Antisense
TCCCTACTTGTGCCCGAATGGAGCA 279 593 799 Antisense
TCAACAACACGGCGCAGTTTCCGCG 505 629 852 Antisense
Sequence Source Affymetrix Proprietary Database