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[189123_at] dehydrogenase

Description F49E10.5 /REP_DB=WormBase Gene ID /WP=CE28436 /TR=Q20596 /GB=AAC69109.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster 6grp_high1_10_10 > 05      6grp_total > 05      4grp_total > 55      6grp_high1_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 297.7
(3)
P 		109.9
(-3)
P 		130
(-2.4)
A 		132
(-2.3)
A 		188
B 246.8
(3)
P 		141.8
(-1.3)
P 		140.9
(-1.3)
P 		99.3
(-3)
A 		148
C 274.4
(3)
P 		147.9
(-1)
P 		127.6
(-1.6)
P 		84.6
(-3)
A 		190
avr 273
(3) 		133.2
(-2) 		132.8
(-2) 		105.3
(-3) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 320
1 2 3 4 5 6 range
A 242.8
(1.7)
P 		278
(3)
P 		184.9
(-0.6)
P 		192.4
(-0.3)
P 		177
(-0.9)
M 		120.8
(-3)
P 		157
B 279
(3)
P 		223.4
(1.2)
P 		201.1
(0.5)
P 		180.3
(-0.2)
P 		161.5
(-0.8)
P 		91.8
(-3)
M 		187
C 412
(3)
P 		227.8
(-0.5)
P 		230.5
(-0.5)
P 		144.3
(-2.1)
P 		209.3
(-0.9)
M 		98.8
(-3)
P 		313
avr 311.3
(3) 		243.1
(1) 		205.5
(-0.1) 		172.3
(-1) 		182.6
(-0.7) 		103.8
(-3) 		208
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 108.4
(3)
P 		23.9
(-2.7)
P 		34
(-2)
A 		19.3
(-3)
A 		89
B 78.7
(3)
P 		35
(-1.6)
P 		32.7
(-1.8)
P 		21.2
(-3)
A 		58
C 90.8
(3)
P 		42.5
(-0.8)
P 		44.5
(-0.6)
P 		14.3
(-3)
A 		77
avr 92.6
(3) 		33.8
(-1.7) 		37.1
(-1.5) 		18.3
(-3) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 44.8
(-1.7)
P 		98.9
(3)
P 		40.3
(-2.1)
P 		48.8
(-1.3)
P 		53.4
(-0.9)
M 		29.4
(-3)
P 		70
B 97.1
(3)
P 		74.4
(0.8)
P 		48.8
(-1.6)
P 		47.8
(-1.7)
P 		65.6
(-0)
P 		34.2
(-3)
M 		63
C 152.7
(3)
P 		69.5
(-1)
P 		58.7
(-1.6)
P 		29
(-3)
P 		67.7
(-1.1)
M 		33.1
(-2.8)
P 		124
avr 98.2
(3) 		80.9
(1.4) 		49.3
(-1.4) 		41.9
(-2.1) 		62.2
(-0.3) 		32.2
(-3) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189123_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16064
Sequence Type Exemplar sequence
Representative Public ID F49E10.5 NCBI
Target Description F49E10.5 /REP_DB=WormBase Gene ID /WP=CE28436 /TR=Q20596 /GB=AAC69109.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076582(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076582 NCBI Caenorhabditis elegans F49E10.5 (dehydrogenase) mRNA, complete cds. 11/11 A 
SNAP00000004444 ENSEMBL cdna:SNAP chromosome:CEL140:X:5869042:5872704:1 11/11 None
GENEFINDER00000004453 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5869042:5872704:1 11/11 None
F49E10.5 ENSEMBL cdna:known chromosome:CEL140:X:5862715:5873278:1 gene:F49E10.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5869041-5872704(+) 100.0 100.0
Public Domain and Genome References
Gene Title dehydrogenase
Ensembl F49E10.5
Entrez Gene 180853 Entrez gene
SwissProt Q20595 EMBL-EBI
WormBase CE29966 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:259438_AT C-terminal binding protein (ANGUSTIFOLIA) at
ATGENOME1:20504_AT C-terminal binding protein (ANGUSTIFOLIA) at
CANINE_2:CFAAFFX.25521.1.S1_AT similar to C-terminal binding protein 1 (CtBP1) (C-terminal binding protein 3) (CtBP3) (50 kDa BFA-dependent ADP-ribosylation substrate) (BARS-50) cfa
CANINE_2:CFAAFFX.25525.1.S1_S_AT similar to C-terminal binding protein 1 (CtBP1) (C-terminal binding protein 3) (CtBP3) (50 kDa BFA-dependent ADP-ribosylation substrate) (BARS-50) cfa
DROSOPHILA_2:1623869_AT C-terminal Binding Protein dm
DROSOPHILA_2:1623106_S_AT C-terminal Binding Protein dm
DROSGENOME1:152826_AT C-terminal Binding Protein dm
CHICKEN:GGA.3495.1.S1_S_AT C-terminal binding protein 1 gga
CHICKEN:GGAAFFX.12741.1.S1_AT C-terminal binding protein 1 gga
HG-U133A_2:203392_S_AT C-terminal binding protein 1 hs
HG-U133A:203392_S_AT C-terminal binding protein 1 hs
HG-U133_PLUS_2:203392_S_AT C-terminal binding protein 1 hs
HG-FOCUS:203392_S_AT C-terminal binding protein 1 hs
HU35KSUBA:RC_AA258801_I_AT C-terminal binding protein 1 hs
HU35KSUBA:RC_AA258801_S_AT C-terminal binding protein 1 hs
HU35KSUBA:U37408_AT C-terminal binding protein 1 hs
HUGENEFL:U37408_AT C-terminal binding protein 1 hs
U133_X3P:G4557496_3P_A_AT C-terminal binding protein 1 hs
U133_X3P:HS2.431402.1.S1_3P_AT C-terminal binding protein 1 hs
U133_X3P:HS.239737.2.A1_3P_X_AT C-terminal binding protein 1 hs
U133_X3P:HS.239737.2.A1_3P_A_AT C-terminal binding protein 1 hs
HG-U133_PLUS_2:1557714_AT C-terminal binding protein 1 hs
HG-U133A_2:212863_X_AT C-terminal binding protein 1 hs
HG-U133_PLUS_2:212863_X_AT C-terminal binding protein 1 hs
HG-U133A:212863_X_AT C-terminal binding protein 1 hs
HG-U133A_2:213980_S_AT C-terminal binding protein 1 hs
HG-U133_PLUS_2:213980_S_AT C-terminal binding protein 1 hs
HG-U133A:213980_S_AT C-terminal binding protein 1 hs
HG-U95AV2:41308_AT C-terminal binding protein 1 hs
HG-U95AV2:41309_G_AT C-terminal binding protein 1 hs
HG-U95B:43382_S_AT C-terminal binding protein 1 hs
HG-U95C:55310_AT C-terminal binding protein 1 hs
HG-U133A_2:213979_S_AT Hypothetical protein LOC285463 hs
HG-U133_PLUS_2:213979_S_AT Hypothetical protein LOC285463 hs
HG-U133A:213979_S_AT Hypothetical protein LOC285463 hs
HG-U133_PLUS_2:243180_AT Hypothetical protein LOC285463 hs
HG-U133B:243180_AT Hypothetical protein LOC285463 hs
HG-U95C:61328_AT Hypothetical protein LOC285463 hs
HG-U95C:61329_G_AT Hypothetical protein LOC285463 hs
HG-U95D:70136_R_AT Hypothetical protein LOC285463 hs
HU35KSUBD:RC_R87296_AT Hypothetical protein LOC285463 hs
U133_X3P:HS.239737.1.A1_3P_AT Hypothetical protein LOC285463 hs
U133_X3P:HS.252854.0.A1_3P_AT Hypothetical protein LOC285463 hs
MOUSE430_2:1415702_A_AT C-terminal binding protein 1 mm
MOE430B:1415702_A_AT C-terminal binding protein 1 mm
MOE430A:1415702_A_AT C-terminal binding protein 1 mm
MOUSE430A_2:1415702_A_AT C-terminal binding protein 1 mm
MG-U74AV2:101081_AT C-terminal binding protein 1 mm
MU11KSUBA:AA271294_S_AT C-terminal binding protein 1 mm
MU19KSUBB:TC29839_AT C-terminal binding protein 1, mRNA (cDNA clone MGC:6995 IMAGE:3155102) mm
RG-U34A:AF067795_AT C-terminal binding protein 1 rn
RG-U34A:AF067795_G_AT C-terminal binding protein 1 rn
RAE230A:1370029_AT C-terminal binding protein 1 rn
RAT230_2:1370029_AT C-terminal binding protein 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6564 L-serine biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
16616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC69110 Hypothetical protein F49E10.5 [Caenorhabditis elegans] ref|NP_508983.2| F49E10.5 [Caenorhabditis elegans] 0.0
blast CAE68813 Hypothetical protein CBG14767 [Caenorhabditis briggsae] 0.0
blast T34290 hypothetical protein F49E10.5 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006612 EMBL-EBI Zinc-finger protein of unknown function DM3 2.5E-16
Pfam IPR006140 EMBL-EBI D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain 1.3E-54
Pfam IPR006139 EMBL-EBI D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain 2.1E-20
Pfam IPR006140 EMBL-EBI D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain 7.0E-16
Pfam IPR006140 EMBL-EBI D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain 1.5E-35
Pfam IPR006139 EMBL-EBI D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain 2.1E-20
Pfam IPR006140 EMBL-EBI D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain 3.9E-54
Sequence
Target Sequence 
>C. ELEGANS:189123_AT
tcagctcatccacttctcaatatgggttttccaacgttgccaaactttccgccaatgtcc
atgagtccgcatttcccttatccaaatccattattagccatgggagctcaaatgggagct
ctcaacccttttatgggcaatggtgcattaccgttcaacccggcggccgccctctcttca
cttgctgcggcgcaggcggcgaatgctcaacggggatcacctgccaatagatcgagtaga
tcaagtcccagtccacacaccaataagtcgtcagtgagtcctggaaacaatggtcatgtc
aagacagagccgtcttcgccggctgcaaaaatcgaggtagatattgccgaaaacgacaag
cattcaatgatgacgtttcttcagcgattaattgctccaaatggagatagtggtgcatcg
acggccgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAGCTCATCCACTTCTCAATATGG 684 623 1228 Antisense
CAATATGGGTTTTCCAACGTTGCCA 466 179 1245 Antisense
CATTTCCCTTATCCAAATCCATTAT 521 215 1297 Antisense
AATGGGAGCTCTCAACCCTTTTATG 470 161 1338 Antisense
GCAATGGTGCATTACCGTTCAACCC 615 321 1364 Antisense
TCAACGGGGATCACCTGCCAATAGA 292 631 1434 Antisense
CCAGTCCACACACCAATAAGTCGTC 239 273 1475 Antisense
TGTCAAGACAGAGCCGTCTTCGCCG 246 559 1524 Antisense
GTCTTCGCCGGCTGCAAAAATCGAG 707 465 1539 Antisense
AATGATGACGTTTCTTCAGCGATTA 280 165 1593 Antisense
GATAGTGGTGCATCGACGGCCGATA 293 425 1633 Antisense
Sequence Source Affymetrix Proprietary Database