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[189122_s_at]

Description B0414.7 /REP_DB=WormBase Gene ID /WP=CE07737 /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase (ST.LOUIS) TR:O01837 protein_id:AAB57718.1
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 65      4grp_high_8_8 > 16      6grp_highest_nonorm > 74      6grp_total > 65      4grp_total > 31      6grp_cgc > 36      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 430
1 2 3 4 range
A 722.2
(3)
P 		301.9
(-3)
P 		320.8
(-2.7)
P 		517.3
(0.1)
P 		420
B 669.3
(3)
P 		316
(-2.6)
M 		292
(-3)
P 		444
(-0.6)
P 		377
C 703.4
(3)
P 		323.7
(-3)
P 		388.2
(-2)
P 		494.4
(-0.3)
P 		380
avr 698.3
(3) 		313.9
(-3) 		333.7
(-2.7) 		485.2
(-0.3) 		384

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 383
1 2 3 4 5 6 range
A 766.9
(1.1)
P 		713.2
(0.1)
P 		655.6
(-0.9)
P 		840.7
(2.4)
P 		537.7
(-3)
P 		874
(3)
P 		336
B 710.9
(1)
P 		527.1
(-2.5)
P 		799.4
(2.7)
P 		734.4
(1.5)
P 		499.4
(-3)
P 		813.1
(3)
P 		314
C 645.2
(-0.8)
P 		721.5
(0.4)
P 		851.3
(2.5)
P 		868
(2.8)
P 		510.8
(-3)
P 		882.8
(3)
P 		372
avr 707.7
(0.4) 		653.9
(-0.6) 		768.8
(1.5) 		814.4
(2.3) 		516
(-3) 		856.6
(3) 		341
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 198
1 2 3 4 range
A 263
(3)
P 		65.5
(-3)
P 		83.9
(-2.4)
P 		75.7
(-2.7)
P 		198
B 213.5
(3)
P 		78
(-2.6)
M 		67.7
(-3)
P 		94.6
(-1.9)
P 		146
C 232.9
(3)
P 		93.1
(-2.6)
P 		135.3
(-0.9)
P 		83.4
(-3)
P 		150
avr 236.5
(3) 		78.9
(-3) 		95.6
(-2.4) 		84.6
(-2.8) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 141.4
(-3)
P 		253.6
(3)
P 		142.8
(-2.9)
P 		213.2
(0.8)
P 		162.2
(-1.9)
P 		212.8
(0.8)
P 		112
B 247.4
(0.4)
P 		175.5
(-3)
P 		194.1
(-2.1)
P 		194.5
(-2.1)
P 		202.9
(-1.7)
P 		303.2
(3)
P 		128
C 239.1
(0.4)
P 		220.1
(-0.5)
P 		216.7
(-0.6)
P 		174.6
(-2.6)
P 		165.3
(-3)
P 		295.5
(3)
P 		130
avr 209.3
(-0.9) 		216.4
(-0.5) 		184.5
(-2.5) 		194.1
(-1.9) 		176.8
(-3) 		270.5
(3) 		94
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189122_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1441
Sequence Type Exemplar sequence
Representative Public ID B0414.7 NCBI
Target Description B0414.7 /REP_DB=WormBase Gene ID /WP=CE07737 /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase (ST.LOUIS) TR:O01837 protein_id:AAB57718.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059282(11), NM_059281(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059282 NCBI Caenorhabditis elegans MTK1/MEKK4 homolog family member (mtk-1) (mtk-1) mRNA, complete cds. 11/11 A 
NM_059281 NCBI Caenorhabditis elegans MTK1/MEKK4 homolog family member (mtk-1) (mtk-1) mRNA, complete cds. 11/11 A 
SNAP00000011714 ENSEMBL cdna:SNAP chromosome:CEL140:I:5803700:5809422:-1 11/11 None
GENEFINDER00000011727 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5803700:5809422:-1 11/11 None
B0414.7a ENSEMBL cdna:known chromosome:CEL140:I:5803544:5809422:-1 gene:B0414.7 11/11 A 
B0414.7b ENSEMBL cdna:known chromosome:CEL140:I:5803608:5809422:-1 gene:B0414.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5803699-5809422(-) 100.0 100.0
Public Domain and Genome References
Ensembl B0414.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB57718 Mtk1/mekk4 homolog protein 1, isoform a [Caenorhabditis elegans] ref|NP_491683.1| MTK1/MEKK4 homolog family member (mtk-1) [Caenorhabditis elegans] 0.0
blast AAK68678 Mtk1/mekk4 homolog protein 1, isoform b [Caenorhabditis elegans] ref|NP_491682.1| MTK1/MEKK4 homolog family member (mtk-1) [Caenorhabditis elegans] 0.0
blast CAE60453 Hypothetical protein CBG04061 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-53
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-53
hanks 2.2.4 CaMK Group; CaMK II KIN1/SNF1/Nim1; p78 4.0E-25
hanks 2.2.4 CaMK Group; CaMK II KIN1/SNF1/Nim1; p78 3.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 8.5E-69
Pfam IPR000719 EMBL-EBI Protein kinase 8.5E-69
Sequence
Target Sequence 
>C. ELEGANS:189122_S_AT
atagcttgttgcgggctgtacagtatcttcactctcataaaatcattcatcgtgatataa
agccagcgaacatattcctcgataagtgtaccgtactcaaattaggagatttcggatgtt
cttcgcgacttgtcgagtcaagtacagtatacggagagtttcaaacaactgccggaactc
ctcaattcatggcaccagaaatttattcatatggggaaaaggatgaaacaactggaagct
atagtggttacggtagaagtgttgacatttgggcacttggagctaccgtagtcaatatga
tgactggaaaagttccattcgaaggacaaactcgtcatcaaatagccttcgcgctttgct
tccgcaagcaaaaaccaacttatcctgatattgccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAGCTTGTTGCGGGCTGTACAGTA 65 23 3683 Antisense
CTGTACAGTATCTTCACTCTCATAA 213 239 3698 Antisense
AAAGCCAGCGAACATATTCCTCGAT 327 125 3741 Antisense
ATTCCTCGATAAGTGTACCGTACTC 681 1 3756 Antisense
GATGTTCTTCGCGACTTGTCGAGTC 674 409 3797 Antisense
GAGAGTTTCAAACAACTGCCGGAAC 250 393 3836 Antisense
TGCCGGAACTCCTCAATTCATGGCA 59 587 3852 Antisense
ACATTTGGGCACTTGGAGCTACCGT 632 103 3947 Antisense
AACTCGTCATCAAATAGCCTTCGCG 437 141 4011 Antisense
TCGCGCTTTGCTTCCGCAAGCAAAA 479 599 4031 Antisense
ACCAACTTATCCTGATATTGCCGAC 205 85 4056 Antisense
Sequence Source Affymetrix Proprietary Database