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[189112_at] protein kinase

Description F59A3.8 /REP_DB=WormBase Gene ID /WP=CE11430 /TR=P91360 /GB=AAB37822.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 89      4grp_total > 13      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 14.7
(-2.1)
A 		10.5
(-3)
A 		39
(3)
A 		36.8
(2.5)
A 		29
B 16.1
(-1.1)
A 		37.9
(2.1)
A 		43.7
(3)
A 		3.4
(-3)
A 		40
C 70.8
(2.6)
A 		35.8
(-0.3)
A 		3.6
(-3)
A 		75.1
(3)
M 		72
avr 33.9
(0.4) 		28.1
(-3) 		28.8
(-2.6) 		38.4
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 56.1
(0.5)
A 		43.1
(-0.4)
A 		4.7
(-3)
A 		18
(-2.1)
A 		68.4
(1.4)
P 		92.4
(3)
P 		88
B 21.7
(-1.2)
A 		17.5
(-1.6)
A 		4
(-3)
A 		38.3
(0.5)
A 		62.5
(3)
A 		58.4
(2.6)
P 		59
C 33.9
(-1.6)
A 		29
(-2.1)
A 		19.6
(-3)
A 		33.4
(-1.7)
A 		75.4
(2.4)
M 		82
(3)
P 		62
avr 37.2
(-0.6) 		29.9
(-1.2) 		9.4
(-3) 		29.9
(-1.2) 		68.8
(2.2) 		77.6
(3) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 5.4
(-0.6)
A 		2.3
(-3)
A 		10.2
(3)
A 		5.4
(-0.6)
A 		8
B 5.1
(-0.2)
A 		9.4
(2.6)
A 		10.1
(3)
A 		0.7
(-3)
A 		9
C 23.4
(3)
A 		10.3
(-0.5)
A 		1.2
(-3)
A 		12.7
(0.1)
M 		22
avr 11.3
(3) 		7.3
(-1.8) 		7.2
(-1.9) 		6.3
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 10.3
(-0.4)
A 		15.3
(1)
A 		1
(-3)
A 		4.6
(-2)
A 		20.6
(2.5)
P 		22.5
(3)
P 		22
B 7.5
(-1.4)
A 		5.8
(-1.8)
A 		1
(-3)
A 		10.1
(-0.8)
A 		25.4
(3)
A 		21.8
(2.1)
P 		24
C 12.6
(-1)
A 		8.8
(-2)
A 		5
(-3)
A 		6.7
(-2.5)
A 		24.4
(2.2)
M 		27.4
(3)
P 		22
avr 10.1
(-0.8) 		10
(-0.9) 		2.3
(-3) 		7.1
(-1.7) 		23.5
(2.9) 		23.9
(3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189112_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2530
Sequence Type Exemplar sequence
Representative Public ID F59A3.8 NCBI
Target Description F59A3.8 /REP_DB=WormBase Gene ID /WP=CE11430 /TR=P91360 /GB=AAB37822.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059219(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059219 NCBI Caenorhabditis elegans F59A3.8 (F59A3.8) mRNA, complete cds. 11/11 None
GENEFINDER00000013461 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5522128:5525276:-1 11/11 None
SNAP00000013445 ENSEMBL cdna:SNAP chromosome:CEL140:I:5522438:5525276:-1 11/11 None
F59A3.8 ENSEMBL cdna:known chromosome:CEL140:I:5522438:5525276:-1 gene:F59A3.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5522437-5525276(-) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl F59A3.8
Entrez Gene 186579 Entrez gene
SwissProt P91360 EMBL-EBI
WormBase CE30549 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37822 Hypothetical protein F59A3.8 [Caenorhabditis elegans] ref|NP_491620.2| F59A3.8 [Caenorhabditis elegans] 0.0
blast CAE67363 Hypothetical protein CBG12830 [Caenorhabditis briggsae] 0.0
blast CAE60040 Hypothetical protein CBG03551 [Caenorhabditis briggsae] 1.0E-143
Protein Families
Method ID Description E-Value
hanks 1.9.5 AGC group; AGC Other; DdK2 1.0E-61
hanks 1.9.5 AGC group; AGC Other; DdK2 7.0E-62
hanks 1.9.5 AGC group; AGC Other; DdK2 6.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 7.9E-62
Pfam IPR000719 EMBL-EBI Protein kinase 7.9E-62
Sequence
Target Sequence 
>C. ELEGANS:189112_AT
ggagttgcagttttacgcgaaccattgtatttgatgattgagttgtgttcgaacggagct
ctacgtgaatatctcaaagaaaatcacaaaacgatcactcttgccgacaagattaacttc
gtactaggttcagctcgtggtgttgcatatcttcattctcaacggattctacatcgagat
ttggcagttcgcaatattcttctcactgaagataaaactccaaaagtgtccgattttggt
ttggctgaattaacggatagatatgaaatgaaagagcattgcaagattcctgtgagatac
ttggcaccggagactctagaactcttcatcttcactccaaaaactgatgttttctcattt
ggatgtgtgatctgggagatctacgagaacggacaacagcctcacgatgggaaaaatgca
cagactattcgagctcaggtcaagaaacgagagttcctgaaactgtcgagttcggcaccg
gaagcacttcgacgactagtggctgagaaggtttttgtagcggatccggaaaaccgatgc
tcgatgacttcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGTTGCAGTTTTACGCGAACCAT 299 517 1177 Antisense
AGTTGTGTTCGAACGGAGCTCTACG 609 61 1217 Antisense
CACAAAACGATCACTCTTGCCGACA 146 195 1261 Antisense
TTCGTACTAGGTTCAGCTCGTGGTG 143 665 1294 Antisense
AGCTCGTGGTGTTGCATATCTTCAT 659 79 1308 Antisense
AGTTCGCAATATTCTTCTCACTGAA 572 63 1362 Antisense
GGAGACTCTAGAACTCTTCATCTTC 279 519 1485 Antisense
CGGACAACAGCCTCACGATGGGAAA 654 239 1566 Antisense
ATGCACAGACTATTCGAGCTCAGGT 455 43 1592 Antisense
CGAGTTCGGCACCGGAAGCACTTCG 564 249 1643 Antisense
GAAAACCGATGCTCGATGACTTCGA 175 355 1705 Antisense
Sequence Source Affymetrix Proprietary Database