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[189110_at]

Description F31E3.2 /REP_DB=WormBase Gene ID /WP=CE01267 /TR=Q19934 /GB=AAA81690.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 07      4grp_high_8_8 > 10      6grp_highest_nonorm > 50      6grp_total > 04      4grp_total > 21      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 657
1 2 3 4 range
A 799.2
(2)
P 		405.2
(-1.6)
A 		249.2
(-3)
A 		905.7
(3)
P 		657
B 695.5
(2.8)
P 		470.8
(-0.2)
P 		263
(-3)
A 		713.6
(3)
P 		451
C 727
(1.8)
P 		430.7
(-1.7)
P 		317
(-3)
P 		833.6
(3)
P 		517
avr 740.6
(2.1) 		435.6
(-1.2) 		276.4
(-3) 		817.6
(3) 		541

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 523
1 2 3 4 5 6 range
A 811.5
(3)
P 		602.7
(0.6)
P 		469.6
(-1)
P 		500.2
(-0.6)
P 		368.8
(-2.2)
P 		296.6
(-3)
P 		515
B 733.1
(3)
P 		526.8
(0.2)
P 		340.7
(-2.3)
P 		518.2
(0.1)
P 		393.1
(-1.6)
P 		292.6
(-3)
P 		441
C 756.5
(3)
P 		519.1
(-0)
P 		490.4
(-0.4)
P 		521.1
(-0)
P 		331.9
(-2.4)
P 		288.8
(-3)
P 		468
avr 767
(3) 		549.5
(0.2) 		433.6
(-1.2) 		513.2
(-0.2) 		364.6
(-2.1) 		292.7
(-3) 		474
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 291
(3)
P 		88
(-2.4)
A 		65.2
(-3)
A 		132.6
(-1.2)
P 		226
B 221.8
(3)
P 		116.3
(-0.9)
P 		61
(-3)
A 		152.1
(0.4)
P 		161
C 240.7
(3)
P 		123.8
(-2.4)
P 		110.5
(-3)
P 		140.6
(-1.6)
P 		130
avr 251.2
(3) 		109.4
(-1.9) 		78.9
(-3) 		141.8
(-0.8) 		172

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 208
1 2 3 4 5 6 range
A 149.6
(0.3)
P 		214.3
(3)
P 		102.3
(-1.7)
P 		126.9
(-0.7)
P 		111.3
(-1.3)
P 		72.2
(-3)
P 		142
B 255.1
(3)
P 		175.3
(0.2)
P 		82.7
(-3)
P 		137.2
(-1.1)
P 		159.7
(-0.3)
P 		109.1
(-2.1)
P 		172
C 280.4
(3)
P 		158.4
(-1)
P 		124.8
(-2.1)
P 		104.8
(-2.7)
P 		107.4
(-2.7)
P 		96.7
(-3)
P 		184
avr 228.4
(3) 		182.7
(1) 		103.3
(-2.5) 		123
(-1.7) 		126.1
(-1.5) 		92.7
(-3) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189110_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6720
Sequence Type Exemplar sequence
Representative Public ID F31E3.2 NCBI
Target Description F31E3.2 /REP_DB=WormBase Gene ID /WP=CE01267 /TR=Q19934 /GB=AAA81690.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066121(11), NM_001027386(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066121 NCBI Caenorhabditis elegans F31E3.2a (F31E3.2) mRNA, complete cds. 11/11 None
NM_001027386 NCBI Caenorhabditis elegans F31E3.2b (F31E3.2) mRNA, complete cds. 11/11 None
GENEFINDER00000009469 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6974393:6977527:1 11/11 None
SNAP00000009457 ENSEMBL cdna:SNAP chromosome:CEL140:III:6976029:6977802:1 11/11 None
F31E3.2a ENSEMBL cdna:known chromosome:CEL140:III:6974274:6977527:1 gene:F31E3.2 11/11 None
F31E3.2b ENSEMBL cdna:known chromosome:CEL140:III:6974393:6977953:1 gene:F31E3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175977 3 2 NM_001027386 189110_at (11)
NM_066121 189110_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6974390-6977525(+) 100.0 100.0
Public Domain and Genome References
Ensembl F31E3.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM29686 Hypothetical protein F31E3.2a [Caenorhabditis elegans] ref|NP_498522.3| F31E3.2a [Caenorhabditis elegans] sp|Q8MYQ1|Y31E_CAEEL Putative serine/threonine-protein kinase F31E3.2 0.0
blast T16220 hypothetical protein F31E3.2 - Caenorhabditis elegans 0.0
blast AAM29687 Hypothetical protein F31E3.2b [Caenorhabditis elegans] ref|NP_001022557.1| F31E3.2b [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
hanks 1.2.5 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; PKCd 4.0E-56
hanks 1.2.5 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; PKCd 1.0E-58
hanks 1.2.5 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; PKCd 6.0E-59
hanks 1.1.15 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); PKG-I 2.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.7E-62
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-55
Pfam IPR000719 EMBL-EBI Protein kinase 1.1E-52
Sequence
Target Sequence 
>C. ELEGANS:189110_AT
tcagctattgatttcctacaccagaatgacgtgatctatcgagatgtaaaactggaaaat
gtagttctagatcaatggggtcacgccttgctaattgattttggtttggcaaaaaagctg
aaacaaggttctagtaccggaacaatatgtggaacattacaatacatgtcacctgacgtg
gcatctggtggaacatattctcattatgttgattggtggtcattaggtgtactcttgcat
attttattaactggaatatatccatatccaaattcagaagccacacatcatgctaattta
aaattcattgactacagtactcctataggatgttcacgtgaattcgccaaccttatggat
agaatgttagcagtttccataacccatcgtttatgctcattcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAGCTATTGATTTCCTACACCAGA 71 625 754 Antisense
TAGATCAATGGGGTCACGCCTTGCT 338 651 821 Antisense
CACGCCTTGCTAATTGATTTTGGTT 25 201 835 Antisense
ACATGTCACCTGACGTGGCATCTGG 162 105 917 Antisense
GTGGCATCTGGTGGAACATATTCTC 629 491 931 Antisense
GGTGGTCATTAGGTGTACTCTTGCA 358 499 968 Antisense
CAGAAGCCACACATCATGCTAATTT 646 203 1028 Antisense
AATTCATTGACTACAGTACTCCTAT 286 177 1055 Antisense
TTCACGTGAATTCGCCAACCTTATG 529 685 1086 Antisense
TTAGCAGTTTCCATAACCCATCGTT 355 681 1120 Antisense
TAACCCATCGTTTATGCTCATTCAC 189 637 1133 Antisense
Sequence Source Affymetrix Proprietary Database