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[189104_s_at] protein kinase

Description W04B5.5 /REP_DB=WormBase Gene ID /WP=CE20142 /TR=Q9UA62 /GB=AAC68917.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 89      4grp_total > 27      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 127.3
(0.1)
P 		155.5
(3)
A 		97.7
(-3)
A 		152.2
(2.7)
A 		58
B 80.6
(-3)
M 		154.1
(3)
A 		92.3
(-2)
A 		94.4
(-1.9)
A 		74
C 136.2
(2.8)
A 		81.8
(-3)
A 		104.2
(-0.6)
P 		138.4
(3)
A 		57
avr 114.7
(0.1) 		130.5
(3) 		98.1
(-3) 		128.3
(2.6) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 61
(-3)
A 		129.6
(1.3)
P 		139.5
(1.9)
A 		114.7
(0.4)
A 		133.9
(1.6)
A 		156.8
(3)
P 		96
B 130.1
(-0)
P 		128.4
(-0.2)
A 		126.4
(-0.3)
A 		90.3
(-3)
A 		171
(3)
A 		152.8
(1.6)
P 		81
C 177.5
(2.3)
P 		140.4
(0.5)
A 		149.6
(0.9)
A 		67.7
(-3)
A 		138.6
(0.4)
P 		192.4
(3)
P 		125
avr 122.9
(-0.5) 		132.8
(0.3) 		138.5
(0.7) 		90.9
(-3) 		147.8
(1.5) 		167.3
(3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 46.3
(3)
P 		33.8
(-0.1)
A 		25.5
(-2.2)
A 		22.3
(-3)
A 		24
B 25.7
(-1.1)
M 		38.1
(3)
A 		21.4
(-2.6)
A 		20.1
(-3)
A 		18
C 45.1
(3)
A 		23.5
(-2.9)
A 		36.3
(0.6)
P 		23.3
(-3)
A 		22
avr 39
(3) 		31.8
(0.5) 		27.7
(-1) 		21.9
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 11.2
(-3)
A 		46.1
(3)
P 		30.4
(0.3)
A 		29.1
(0.1)
A 		40.4
(2)
A 		38.2
(1.6)
P 		35
B 45.3
(-0.2)
P 		42.7
(-0.5)
A 		30.7
(-2.1)
A 		23.9
(-3)
A 		69.5
(3)
A 		57
(1.4)
P 		46
C 65.8
(3)
P 		42.8
(0.4)
A 		38.1
(-0.2)
A 		13.6
(-3)
A 		44.9
(0.6)
P 		64.4
(2.8)
P 		52
avr 40.8
(0.6) 		43.9
(1.2) 		33.1
(-0.9) 		22.2
(-3) 		51.6
(2.7) 		53.2
(3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189104_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6385
Sequence Type Exemplar sequence
Representative Public ID W04B5.5 NCBI
Target Description W04B5.5 /REP_DB=WormBase Gene ID /WP=CE20142 /TR=Q9UA62 /GB=AAC68917.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065150(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065150 NCBI Caenorhabditis elegans W04B5.5 (W04B5.5) mRNA, complete cds. 11/11 None
SNAP00000019369 ENSEMBL cdna:SNAP chromosome:CEL140:III:2411233:2424287:-1 11/11 None
W04B5.5 ENSEMBL cdna:known chromosome:CEL140:III:2411233:2418193:-1 gene:W04B5.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000019382 4/11 Cross Hyb Matching Probes None
GENEFINDER00000019380 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2411232-2418179(-) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl W04B5.5
Entrez Gene 175360 Entrez gene
SwissProt Q9UA62 EMBL-EBI
WormBase CE20142 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HU35KSUBC:RC_AA121995_AT similar to 3-phosphoinositide dependent protein kinase-1 (hPDK1) hs
HG-U95C:57806_AT similar to 3-phosphoinositide dependent protein kinase-1 (hPDK1) hs
HG-U95E:73168_S_AT similar to 3-phosphoinositide dependent protein kinase-1 (hPDK1) hs
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC68917 Hypothetical protein W04B5.5 [Caenorhabditis elegans] ref|NP_497551.1| W04B5.5 [Caenorhabditis elegans] 0.0
blast AAC68917 Hypothetical protein W04B5.5 [Caenorhabditis elegans] ref|NP_497551.1| W04B5.5 [Caenorhabditis elegans] 1.0E-140
blast CAE69464 Hypothetical protein CBG15660 [Caenorhabditis briggsae] 1.0E-137
blast CAE69464 Hypothetical protein CBG15660 [Caenorhabditis briggsae] 1.0E-109
blast XP_394208 PREDICTED: similar to CG1210-PC, isoform C [Apis mellifera] 2.0E-56
blast XP_394208 PREDICTED: similar to CG1210-PC, isoform C [Apis mellifera] 1.0E-46
Protein Families
Method ID Description E-Value
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 1.0E-57
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 1.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-70
Pfam IPR000719 EMBL-EBI Protein kinase 2.0E-58
Sequence
Target Sequence 
>C. ELEGANS:189104_S_AT
cggattccaagttcaacgtcgcagattctcgatattatgcggccaatttgctgtcagcac
ttgaacacattcacaaattgggcataattcatcgagatgtgaaagcggacaatttattgg
tgaaatcagatggaagaatcatgttaaccgattttggatcatcgaaatttctgtcagatt
atcagaaaattcaggaaaatcctgtggaagaagagcagccgacggggcggcggagcagtt
ttgttggaaccgcgttttttgtgacacctgagctgctgaccggctccgaaatgagcccat
ccagtgatttatgggcattctccgtgacattgtaccttttcctaacaggaatctatccgt
tcaacgatatgtcggaatatctcgtttttcgacggattcaagacattttatacacttttt
ccgaagattttcccgatgaaaatgcaaaaaatttgattgagcggcttttggttaaagagc
aaaaatcccgtctgacaagccaggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGATTCCAAGTTCAACGTCGCAGA 666 243 395 Antisense
CAGATTCTCGATATTATGCGGCCAA 696 205 416 Antisense
ATGCGGCCAATTTGCTGTCAGCACT 72 43 431 Antisense
GCTGTCAGCACTTGAACACATTCAC 668 295 444 Antisense
GGGCGGCGGAGCAGTTTTGTTGGAA 359 373 619 Antisense
ACCGCGTTTTTTGTGACACCTGAGC 73 89 643 Antisense
GACCGGCTCCGAAATGAGCCCATCC 507 379 672 Antisense
GATTTATGGGCATTCTCCGTGACAT 597 427 700 Antisense
TCCGTGACATTGTACCTTTTCCTAA 587 593 715 Antisense
GAATATCTCGTTTTTCGACGGATTC 605 325 769 Antisense
AAAATCCCGTCTGACAAGCCAGGAA 643 131 876 Antisense
Sequence Source Affymetrix Proprietary Database