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[189101_at] GTPase-activating protein

Description C04D8.1 /REP_DB=WormBase Gene ID /WP=CE26357 /GB=AAK71357.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=GTPase-activating protein
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 18      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 30      6grp_total > 06      4grp_total > 46      6grp_highest_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 960
1 2 3 4 range
A 1360
(3)
P 		720.2
(-1.7)
P 		536.6
(-3)
P 		556.6
(-2.9)
P 		823
B 1342.2
(3)
P 		753.9
(-1.1)
P 		474.1
(-3)
P 		654.5
(-1.8)
P 		868
C 1373.8
(3)
P 		757.5
(-0.9)
P 		610.4
(-1.8)
P 		413.7
(-3)
P 		960
avr 1358.7
(3) 		743.9
(-1.5) 		540.4
(-3) 		541.6
(-3) 		818

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1144
1 2 3 4 5 6 range
A 1040.9
(2.4)
P 		1113.2
(3)
P 		404.2
(-3)
P 		594.1
(-1.4)
P 		699.9
(-0.5)
P 		790.7
(0.3)
P 		709
B 1008.4
(3)
P 		835.6
(0.9)
P 		721.9
(-0.4)
P 		672.3
(-1)
P 		637.5
(-1.4)
P 		506.5
(-3)
P 		502
C 1548.6
(3)
P 		804.1
(-1.2)
P 		730
(-1.6)
P 		609.1
(-2.3)
P 		790.4
(-1.3)
P 		485
(-3)
P 		1064
avr 1199.3
(3) 		917.6
(0.2) 		618.7
(-2.8) 		625.2
(-2.7) 		709.3
(-1.9) 		594.1
(-3) 		605
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 425
1 2 3 4 range
A 495.2
(3)
P 		156.3
(-1.9)
P 		140.3
(-2.1)
P 		81.5
(-3)
P 		414
B 428
(3)
P 		186.2
(-1.6)
P 		109.9
(-3)
P 		139.5
(-2.4)
P 		318
C 454.8
(3)
P 		217.8
(-0.7)
P 		212.8
(-0.8)
P 		69.8
(-3)
P 		385
avr 459.3
(3) 		186.8
(-1.5) 		154.3
(-2) 		96.9
(-3) 		362

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 486
1 2 3 4 5 6 range
A 191.9
(-1)
P 		395.9
(3)
P 		88
(-3)
P 		150.7
(-1.8)
P 		211.2
(-0.6)
P 		192.5
(-1)
P 		308
B 350.9
(3)
P 		278.1
(0.5)
P 		175.3
(-3)
P 		178.1
(-2.9)
P 		259
(-0.1)
P 		188.9
(-2.5)
P 		176
C 574
(3)
P 		245.4
(-1.4)
P 		185.8
(-2.2)
P 		122.5
(-3)
P 		255.8
(-1.2)
P 		162.4
(-2.5)
P 		452
avr 372.3
(3) 		306.5
(1.2) 		149.7
(-3) 		150.4
(-3) 		242
(-0.5) 		181.3
(-2.1) 		223
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189101_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7606
Sequence Type Exemplar sequence
Representative Public ID C04D8.1 NCBI
Target Description C04D8.1 /REP_DB=WormBase Gene ID /WP=CE26357 /GB=AAK71357.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=GTPase-activating protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066476(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066476 NCBI Caenorhabditis elegans C04D8.1 (C04D8.1) mRNA, complete cds. 11/11 None
SNAP00000000300 ENSEMBL cdna:SNAP chromosome:CEL140:III:8512732:8518311:1 11/11 None
GENEFINDER00000000311 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8513734:8518311:1 11/11 None
C04D8.1 ENSEMBL cdna:known chromosome:CEL140:III:8497536:8518789:1 gene:C04D8.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8497677-8518306(+) 98.9 100.0
Public Domain and Genome References
Gene Title GTPase-activating protein
Ensembl C04D8.1
Entrez Gene 176201 Entrez gene
SwissProt P34288 EMBL-EBI
WormBase CE33028 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK71357 Hypothetical protein C04D8.1 [Caenorhabditis elegans] ref|NP_498877.3| C04D8.1 [Caenorhabditis elegans] sp|P34288|GAP_CAEEL GTPase-activating protein GAP (CeGAP) 0.0
blast AAA18934 GTPase-activating protein 0.0
blast CAE71244 Hypothetical protein CBG18119 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000198 EMBL-EBI RhoGAP domain 3.1E-71
Pfam IPR000198 EMBL-EBI RhoGAP domain 3.1E-71
Pfam IPR001849 EMBL-EBI Pleckstrin-like 4.0E-18
Pfam IPR000198 EMBL-EBI RhoGAP domain 3.1E-71
Sequence
Target Sequence 
>C. ELEGANS:189101_AT
accaaagatctcaccgatgctctgtcatgtacttctgattattcgacgacaagttctgct
ccacttagtacaaatccaccattggctgttgcttgtgctgatcaacctaactcaagctcc
gactatgcttctagtgatccctcaccttgtgccagaaatccatccacctctcctgcttcc
cgtccctcgaatcttgcaatttctccagctcaactccatgccacttcatcatccggacag
tcacatcaaccaatgagcagaagtcaaaagattcgattaagaactaaactcggttcccgt
gatcctgccagacgacacacgttaagcgatgttgatactttgaaggaaggccgccttgac
aagcttgcgcgctggttcggaatcaggaaatctagcccagacgtgagccgtgatgaggtg
tcagacgatgaaaagaaccatcaagaagcgcctcctctacctgctgcagcaccaccagtt
attgttcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCAAAGATCTCACCGATGCTCTGT 197 85 3643 Antisense
GATGCTCTGTCATGTACTTCTGATT 107 415 3658 Antisense
TTATTCGACGACAAGTTCTGCTCCA 396 679 3681 Antisense
GCTCCACTTAGTACAAATCCACCAT 507 295 3700 Antisense
AATCCACCATTGGCTGTTGCTTGTG 599 167 3715 Antisense
TGCTTGTGCTGATCAACCTAACTCA 178 579 3732 Antisense
ACCTAACTCAAGCTCCGACTATGCT 338 87 3747 Antisense
CGTGATCCTGCCAGACGACACACGT 525 245 3940 Antisense
ATCTAGCCCAGACGTGAGCCGTGAT 66 33 4032 Antisense
AAAGAACCATCAAGAAGCGCCTCCT 370 123 4074 Antisense
GCAGCACCACCAGTTATTGTTCGAA 706 311 4108 Antisense
Sequence Source Affymetrix Proprietary Database