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[189098_at] cytochrome P450

Description K05D4.4 /REP_DB=WormBase Gene ID /WP=CE16230 /TR=O45659 /GB=CAB07250.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 08      4grp_total > 35      6grp_cgc > 20      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 10.6
(-3)
A 		52.4
(3)
A 		13.9
(-2.5)
A 		15.8
(-2.3)
A 		42
B 50.2
(2.7)
A 		51.2
(3)
A 		32
(-3)
A 		34.4
(-2.3)
A 		19
C 13.8
(-3)
A 		23.6
(-1.9)
A 		13.7
(-3)
A 		68.1
(3)
A 		54
avr 24.9
(-1.7) 		42.4
(3) 		19.9
(-3) 		39.4
(2.2) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 54
(3)
A 		18.1
(-1.9)
A 		9.7
(-3)
A 		19.6
(-1.7)
A 		15.5
(-2.2)
A 		13.3
(-2.5)
A 		44
B 13.6
(-2.1)
A 		30.3
(-0.1)
A 		56.2
(3)
A 		18.3
(-1.5)
A 		5.9
(-3)
A 		23.5
(-0.9)
A 		50
C 67.4
(3)
A 		31.9
(-1)
A 		13.6
(-3)
A 		60.4
(2.2)
A 		14.9
(-2.9)
A 		22.6
(-2)
A 		54
avr 45
(3) 		26.8
(-0.3) 		26.5
(-0.4) 		32.8
(0.8) 		12.1
(-3) 		19.8
(-1.6) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 3.9
(-1.9)
A 		11.4
(3)
A 		3.6
(-2.1)
A 		2.3
(-3)
A 		9
B 16
(3)
A 		12.6
(0.7)
A 		7.4
(-2.9)
A 		7.3
(-3)
A 		9
C 4.6
(-3)
A 		6.8
(-1.1)
A 		4.8
(-2.8)
A 		11.5
(3)
A 		7
avr 8.2
(0.5) 		10.3
(3) 		5.3
(-3) 		7
(-1) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 10
(3)
A 		6.4
(0.3)
A 		2.1
(-3)
A 		5
(-0.8)
A 		4.7
(-1)
A 		3.2
(-2.2)
A 		8
B 4.7
(-1.8)
A 		10.1
(1.1)
A 		13.7
(3)
A 		4.8
(-1.7)
A 		2.4
(-3)
A 		8.8
(0.4)
A 		11
C 25
(3)
A 		9.7
(-1.3)
A 		3.5
(-3)
A 		12.2
(-0.6)
A 		4.8
(-2.6)
A 		7.5
(-1.9)
A 		22
avr 13.2
(3) 		8.7
(0.1) 		6.4
(-1.4) 		7.3
(-0.8) 		4
(-3) 		6.5
(-1.4) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189098_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12947
Sequence Type Exemplar sequence
Representative Public ID K05D4.4 NCBI
Target Description K05D4.4 /REP_DB=WormBase Gene ID /WP=CE16230 /TR=O45659 /GB=CAB07250.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074675(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074675 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33D1) (cyp-33D1) mRNA, complete cds. 11/11 None
SNAP00000023574 ENSEMBL cdna:SNAP chromosome:CEL140:V:16176410:16178017:-1 11/11 None
GENEFINDER00000023578 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16176410:16178403:-1 11/11 None
K05D4.4 ENSEMBL cdna:known chromosome:CEL140:V:16176410:16178403:-1 gene:K05D4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16176409-16178403(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-33D1
Ensembl K05D4.4
Entrez Gene 187025 Entrez gene
SwissProt O45659 EMBL-EBI
WormBase CE16230 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246984_AT cytochrome P450 family protein at
CANINE:1601487_AT similar to cytochrome P450, family 2, subfamily J, polypeptide 2 cfa
CANINE_2:CFAAFFX.28812.1.S1_AT similar to cytochrome P450, family 2, subfamily J, polypeptide 2 cfa
DROSOPHILA_2:1628584_AT dm
DROSGENOME1:153050_AT dm
HG-U95AV2:500_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HC-G110:500_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HG-U95AV2:501_G_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HC-G110:501_G_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HG-U133_PLUS_2:205073_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HG-U133A:205073_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HG-FOCUS:205073_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HG-U133A_2:205073_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HU35KSUBD:RC_N53549_S_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
HUGENEFL:U37143_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
U133_X3P:G4503226_3P_AT cytochrome P450, family 2, subfamily J, polypeptide 2 hs
MOUSE430A_2:1417952_AT cytochrome P450, family 2, subfamily j, polypeptide 6 mm
MOE430A:1417952_AT cytochrome P450, family 2, subfamily j, polypeptide 6 mm
MOUSE430_2:1417952_AT cytochrome P450, family 2, subfamily j, polypeptide 6 mm
MU11KSUBA:U62295_S_AT cytochrome P450, family 2, subfamily j, polypeptide 6 mm
MG-U74AV2:92813_AT cytochrome P450, family 2, subfamily j, polypeptide 6 mm
MOE430B:1440691_AT cytochrome P450, family 2, subfamily j, polypeptide 6 mm
MOUSE430_2:1440691_AT cytochrome P450, family 2, subfamily j, polypeptide 6 mm
MU19KSUBA:TC25778_AT Cytochrome P450, family 2, subfamily j, polypeptide 6 (Cyp2j6), mRNA mm
RAE230A:1387296_AT cytochrome P450, family 2, subfamily J, polypeptide 4 rn
RAT230_2:1387296_AT cytochrome P450, family 2, subfamily J, polypeptide 4 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07250 Hypothetical protein K05D4.4 [Caenorhabditis elegans] ref|NP_507076.1| CYtochrome P450 family member (cyp-33D1) [Caenorhabditis elegans] 0.0
blast CAB04001 Hypothetical protein Y17D7A.4 [Caenorhabditis elegans] emb|CAA16294.2| Hypothetical protein Y17D7A.4 [Caenorhabditis elegans] ref|NP_507679.2| CYtochrome P450 family member (cyp-33D3) [Caenorhabditis elegans] 0.0
blast CAE63115 Hypothetical protein CBG07412 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
p450 CPA7_HUMAN Animalia; CYP2A7; hum; CPA7_HUMAN; Homo sapiens; P20853 1.0E-121
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.7E-104
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.7E-81
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507076.1 1 2-20
K05D4.4 1 2-20
GENEFINDER00000023578 1 2-20
Sequence
Target Sequence 
>C. ELEGANS:189098_AT
gtgttttgatctctggatggccggacttcagacaaccacagtcacccttacctggggatt
ctcattttatcttcataacgccgacgtacagctgaaaattcgagaagagctagacagagt
cattggtaatgatagacttatcaccactgctgataagaattgcttaccttatcttacggc
ttttatcaatgaaactcaacgttgcgcaaatataattcctttcaatttgctacacgtcgc
aactagggatactgttattgaaggataccccgtgaaaaaggggacaggcgtcattgcaca
aattgggacagtcatgtcggatgagcagatcttccccgacgcccattgcttcaatccggg
ccgcttcattgaaaacggcaagctgaaaaaagtcgacgaagtgatcccgttctcaattgg
aaaacgacaatgtttgggagaaggtcttgcgcgaatggagcttttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTTTTGATCTCTGGATGGCCGGA 610 485 888 Antisense
GGACTTCAGACAACCACAGTCACCC 81 525 910 Antisense
TCACCCTTACCTGGGGATTCTCATT 584 625 929 Antisense
ATCTTCATAACGCCGACGTACAGCT 408 29 956 Antisense
AATTGCTTACCTTATCTTACGGCTT 128 177 1045 Antisense
TTCCTTTCAATTTGCTACACGTCGC 636 699 1103 Antisense
AGGGGACAGGCGTCATTGCACAAAT 122 57 1166 Antisense
ATGTCGGATGAGCAGATCTTCCCCG 153 49 1201 Antisense
TCAATCCGGGCCGCTTCATTGAAAA 486 631 1238 Antisense
AGTCGACGAAGTGATCCCGTTCTCA 638 59 1278 Antisense
AGGTCTTGCGCGAATGGAGCTTTTC 606 57 1329 Antisense
Sequence Source Affymetrix Proprietary Database