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[189094_at] 6-phosphofructo-2-kinase

Description K02B2.1 /REP_DB=WormBase Gene ID /WP=CE04687 /GB=AAK39245.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=6-phosphofructo-2-kinase
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 96      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 97      6grp_total > 86      4grp_total > 61      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 558
1 2 3 4 range
A 826.1
(3)
P 		809.6
(2.8)
P 		766.5
(2.2)
P 		376.1
(-3)
P 		450
B 911.8
(3)
P 		866.5
(2.4)
P 		772.6
(1.3)
P 		422.8
(-3)
P 		489
C 934.2
(3)
P 		548.7
(-3)
P 		869.4
(2)
P 		602.8
(-2.2)
P 		386
avr 890.7
(3) 		741.6
(0.9) 		802.8
(1.8) 		467.2
(-3) 		424

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 986
1 2 3 4 5 6 range
A 638.7
(-3)
P 		887
(-1.3)
P 		888.3
(-1.3)
P 		1171.1
(0.6)
P 		996.5
(-0.6)
P 		1533.5
(3)
P 		895
B 547.1
(-3)
P 		815.6
(-0.7)
P 		743.4
(-1.3)
P 		1067.1
(1.5)
P 		1058.8
(1.4)
P 		1240.8
(3)
P 		694
C 925.5
(-1.4)
P 		1000.8
(-0.5)
P 		996.3
(-0.6)
P 		791
(-3)
P 		1146.1
(1.2)
P 		1297.4
(3)
P 		506
avr 703.8
(-3) 		901.1
(-1.2) 		876
(-1.4) 		1009.7
(-0.2) 		1067.1
(0.3) 		1357.2
(3) 		653
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 254
1 2 3 4 range
A 300.8
(3)
P 		175.7
(-0.1)
P 		200.4
(0.5)
P 		55.1
(-3)
P 		246
B 290.8
(3)
P 		214
(0.7)
P 		179
(-0.3)
P 		90.1
(-3)
P 		201
C 309.2
(3)
P 		157.8
(-1.4)
P 		303
(2.8)
P 		101.6
(-3)
P 		208
avr 300.3
(3) 		182.5
(-0.2) 		227.5
(1) 		82.3
(-3) 		218

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 345
1 2 3 4 5 6 range
A 117.7
(-3)
P 		315.5
(1.6)
P 		193.5
(-1.2)
P 		297
(1.2)
P 		300.7
(1.3)
P 		373.3
(3)
P 		256
B 190.4
(-2.8)
P 		271.5
(-1.1)
P 		180.5
(-3)
P 		282.6
(-0.8)
P 		430.2
(2.3)
P 		462.7
(3)
P 		282
C 343
(1)
P 		305.4
(0.2)
P 		253.6
(-0.9)
P 		159.1
(-3)
P 		370.8
(1.6)
P 		434.3
(3)
P 		275
avr 217
(-2.8) 		297.5
(-0.5) 		209.2
(-3) 		246.2
(-2) 		367.2
(1.4) 		423.4
(3) 		214
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189094_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11080
Sequence Type Exemplar sequence
Representative Public ID K02B2.1 NCBI
Target Description K02B2.1 /REP_DB=WormBase Gene ID /WP=CE04687 /GB=AAK39245.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=6-phosphofructo-2-kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068492(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068492 NCBI Caenorhabditis elegans K02B2.1 (6-phosphofructo-2-kinase) mRNA, complete cds. 11/11 None
SNAP00000017500 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5941916:5943811:1 11/11 None
GENEFINDER00000017506 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5941916:5943811:1 11/11 None
K02B2.1 ENSEMBL cdna:known chromosome:CEL140:IV:5941910:5943976:1 gene:K02B2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5941921-5943817(+) 100.0 100.0
Public Domain and Genome References
Gene Title 6-phosphofructo-2-kinase
Ensembl K02B2.1
Entrez Gene 177363 Entrez gene
SwissProt Q21122 EMBL-EBI
WormBase CE30082 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256036_AT fructose-6-phosphate 2-kinase / fructose-2,6-bisphosphatase (F2KP) at
ATGENOME1:18004_AT fructose-6-phosphate 2-kinase / fructose-2,6-bisphosphatase (F2KP) at
CANINE:1584341_AT similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (6PF-2-K/Fru-2,6-P2ASE brain-type isozyme) (RB2K) cfa
CANINE_2:CFA.10187.1.A1_AT similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (6PF-2-K/Fru-2,6-P2ASE brain-type isozyme) (RB2K) cfa
CANINE_2:CFA.21468.1.S1_AT similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (6PF-2-K/Fru-2,6-P2ASE brain-type isozyme) (RB2K) cfa
CANINE_2:CFAAFFX.8751.1.S1_AT similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (6PF-2-K/Fru-2,6-P2ASE brain-type isozyme) (RB2K) cfa
CANINE_2:CFAAFFX.8751.1.S1_S_AT similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (6PF-2-K/Fru-2,6-P2ASE brain-type isozyme) (RB2K) cfa
DROSOPHILA_2:1633454_S_AT 6-phosphofructo-2-kinase dm
DROSGENOME1:152692_AT 6-phosphofructo-2-kinase dm
CHICKEN:GGA.10885.1.S1_S_AT similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; inducible 6-phosphofructo-2-kinase gga
CHICKEN:GGAAFFX.21904.1.S1_AT similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; inducible 6-phosphofructo-2-kinase gga
HG-U95AV2:39522_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HG-U95AV2:37110_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HG-FOCUS:202464_S_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HG-U133A_2:202464_S_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HG-U133A:202464_S_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HG-U133_PLUS_2:202464_S_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HU35KSUBA:D49817_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HUGENEFL:D49817_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HU35KSUBA:RC_AA282405_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
U133_X3P:G4758899_3P_A_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HG-U95AV2:37111_G_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
HG-U95B:56246_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 hs
MOUSE430A_2:1456676_A_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MOE430A:1456676_A_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MOUSE430_2:1456676_A_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MU11KSUBA:AA163244_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MU11KSUBB:MSA.32178.0_S_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MG-U74AV2:103577_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MG-U74AV2:160641_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MOE430A:1416432_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MOUSE430A_2:1416432_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MOUSE430_2:1416432_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 mm
MU19KSUBB:TC32668_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant 1, mRNA mm
RG-U34A:D87240_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 rn
RG-U34A:AB006710_S_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 rn
RAE230A:1369794_A_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 rn
RAT230_2:1369794_A_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 rn
RAE230B:1397082_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 rn
RAT230_2:1397082_AT 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 rn
YEAST_2:1774340_AT Fructose-2,6-bisphosphatase, required for glucose metabolism Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6003 fructose 2,6-bisphosphate metabolism inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK39245 Hypothetical protein K02B2.1 [Caenorhabditis elegans] ref|NP_500893.2| K02B2.1 [Caenorhabditis elegans] sp|Q21122|F26_CAEEL Putative 6PF-2-K/Fru-2,6-P2ASE [Includes: 6-phosphofructo-2-kinase ; Fructose-2,6-bisphosphatase ] 0.0
blast C88700 protein K02B2.1 [imported] - Caenorhabditis elegans 0.0
blast CAE64683 Hypothetical protein CBG09461 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec 6P21_YEAST (P40433) 6-phosphofructo-2-kinase 1 (EC 2.7.1.105) (Phosphofructokinase 2 I) (6PF-2-K 1) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003094 EMBL-EBI Fructose-2,6-bisphosphatase 1.0E-126
Pfam IPR001345 EMBL-EBI Phosphoglycerate/bisphosphoglycerate mutase 1.9E-95
Sequence
Target Sequence 
>C. ELEGANS:189094_AT
acttgacgaggcacggacaatccgaatataacgctatgggacgtctcggaggcgattccc
cacttacagaagacggtcaaaagtatgcatctgctctcgctgatttcttcgaagaagagg
aagtaccgggacttcgtgtttggtgttcacaaaaagtgagagctgctcaaactgctcaac
atttgaaaccagatttccatactgaatactggaaggcactcgatgaactggatgctggaa
tttgtgaaggattgacttacgaagatattctacagagatatccgaagcaagcagatgatc
gagcaactgataagtatcattatcggtatccaagtggtgaaagttatgaagatgtcgtta
gtcggttggagccggtgatcatggagctggaaagacaagcgaatgtccttgtggtttcac
atcaagcagttcttcgatgtgtactcgcatatttctatgatcgtccactttctgagttac
catacattgatattccacttcattctttggtcaaactgactccacgtgcctaccattgtg
attcgactat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTGACGAGGCACGGACAATCCGA 487 103 737 Antisense
TATGGGACGTCTCGGAGGCGATTCC 11 655 771 Antisense
GATTCCCCACTTACAGAAGACGGTC 234 431 790 Antisense
AAAAGTATGCATCTGCTCTCGCTGA 293 133 815 Antisense
GTGAGAGCTGCTCAAACTGCTCAAC 699 481 892 Antisense
AGATGTCGTTAGTCGGTTGGAGCCG 284 67 1086 Antisense
AAGCGAATGTCCTTGTGGTTTCACA 31 151 1133 Antisense
GCAGTTCTTCGATGTGTACTCGCAT 160 313 1162 Antisense
TGATCGTCCACTTTCTGAGTTACCA 599 565 1194 Antisense
GATATTCCACTTCATTCTTTGGTCA 6 427 1225 Antisense
GTGCCTACCATTGTGATTCGACTAT 16 479 1262 Antisense
Sequence Source Affymetrix Proprietary Database