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[189091_at] phospholipase

Description C03H5.4 /REP_DB=WormBase Gene ID /WP=CE07897 /TR=O16654 /GB=AAB66132.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=phospholipase
Cluster 6grp_total > 99      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 2.1
(-3)
A 		3.6
(-2.9)
A 		3
(-2.9)
A 		77.4
(3)
A 		75
B 4.1
(-2.6)
A 		4.6
(-2.5)
A 		1.8
(-3)
A 		35.5
(3)
A 		34
C 7.4
(-2.6)
A 		9.1
(-2.5)
A 		1.8
(-3)
A 		88
(3)
A 		86
avr 4.5
(-2.8) 		5.8
(-2.7) 		2.2
(-3) 		67
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 5.7
(-2.7)
A 		1.4
(-3)
A 		20.4
(-1.7)
A 		8.8
(-2.5)
A 		82.6
(2.5)
M 		90.1
(3)
P 		89
B 5.4
(-3)
A 		4.8
(-3)
A 		15.3
(-2.5)
A 		33.7
(-1.5)
A 		122.2
(3)
P 		94.6
(1.6)
P 		117
C 2.6
(-2.9)
A 		3.7
(-2.9)
A 		1.5
(-3)
A 		7.1
(-2.7)
A 		85.9
(0.8)
P 		133.1
(3)
P 		132
avr 4.6
(-2.9) 		3.3
(-3) 		12.4
(-2.5) 		16.5
(-2.2) 		96.9
(2.5) 		105.9
(3) 		103
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 0.8
(-3)
A 		0.8
(-3)
A 		0.8
(-3)
A 		11.3
(3)
A 		11
B 1.3
(-2.3)
A 		1.1
(-2.4)
A 		0.4
(-3)
A 		7.6
(3)
A 		7
C 2.5
(-2.2)
A 		2.6
(-2.2)
A 		0.6
(-3)
A 		14.8
(3)
A 		14
avr 1.5
(-2.5) 		1.5
(-2.5) 		0.6
(-3) 		11.2
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 1.1
(-2.9)
A 		0.5
(-3)
A 		4.4
(-2)
A 		2.2
(-2.6)
A 		24.9
(3)
M 		21.9
(2.3)
P 		24
B 1.9
(-3)
A 		1.6
(-3)
A 		3.7
(-2.7)
A 		8.9
(-2.1)
A 		49.6
(3)
P 		35.3
(1.2)
P 		48
C 1
(-2.9)
A 		1.1
(-2.9)
A 		0.4
(-3)
A 		1.4
(-2.9)
A 		27.8
(0.7)
P 		44.6
(3)
P 		44
avr 1.3
(-3) 		1.1
(-3) 		2.8
(-2.7) 		4.2
(-2.4) 		34.1
(3) 		33.9
(3) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189091_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5568
Sequence Type Exemplar sequence
Representative Public ID C03H5.4 NCBI
Target Description C03H5.4 /REP_DB=WormBase Gene ID /WP=CE07897 /TR=O16654 /GB=AAB66132.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=phospholipase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061318(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061318 NCBI Caenorhabditis elegans C03H5.4 (phospholipase) mRNA, complete cds. 11/11 None
GENEFINDER00000020974 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:375249:380412:1 11/11 None
C03H5.4 ENSEMBL cdna:known chromosome:CEL140:II:375249:375834:1 gene:C03H5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:375248-375834(+) 100.0 100.0
Public Domain and Genome References
Gene Title phospholipase
Ensembl C03H5.4
Entrez Gene 182181 Entrez gene
SwissProt O16654 EMBL-EBI
WormBase CE07897 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
16042 lipid catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4623 phospholipase A2 activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66132 Hypothetical protein C03H5.4 [Caenorhabditis elegans] ref|NP_493719.1| C03H5.4 [Caenorhabditis elegans] 3.0E-73
blast AAB66132 Hypothetical protein C03H5.4 [Caenorhabditis elegans] ref|NP_493719.1| C03H5.4 [Caenorhabditis elegans] 8.0E-71
blast CAE68100 Hypothetical protein CBG13740 [Caenorhabditis briggsae] 4.0E-22
blast CAE59379 Hypothetical protein CBG02732 [Caenorhabditis briggsae] 2.0E-14
blast CAA90098 Hypothetical protein C07E3.9 [Caenorhabditis elegans] ref|NP_496228.1| C07E3.9 [Caenorhabditis elegans] 1.0E-13
blast CAE59379 Hypothetical protein CBG02732 [Caenorhabditis briggsae] 2.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR001211 EMBL-EBI Phospholipase A2 2.3E-19
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000020974 5 273-295,307-329,349-371,392-414,455-477
Sequence
Target Sequence 
>C. ELEGANS:189091_AT
ggggtttttgctgccagaaactctgaatacttttgtactcgaaatccaatttatgacacg
ttgtatgacgcaccatgatgcttggatttacaacggctacgggtgttattgtggaattgg
aggtagtggagacattttagatgatattgacgaatgctgtgctaaccatgacaaatgcta
tgaagaccttgatctcaaaaaaacatgctggacaactccatttgaatactttccaattta
ctcctggaaatgtcaaaatcaaactattcactgtgatacactaagtgaggcatctgatca
tccagtcttagctcacgagtgcagtaatcaattatgtgaatgtgatcgaaaactcgtgga
gtgttgggcaaagtacccggagccaacaggacagctaaagtgcccacatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGGTTTTTGCTGCCAGAAACTCTG 216 491 36 Antisense
GACACGTTGTATGACGCACCATGAT 205 367 90 Antisense
GATGCTTGGATTTACAACGGCTACG 694 407 112 Antisense
ACGGCTACGGGTGTTATTGTGGAAT 65 95 128 Antisense
GACGAATGCTGTGCTAACCATGACA 348 379 184 Antisense
GACAACTCCATTTGAATACTTTCCA 413 365 246 Antisense
GAATACTTTCCAATTTACTCCTGGA 215 327 259 Antisense
GTGAGGCATCTGATCATCCAGTCTT 560 483 320 Antisense
CATCCAGTCTTAGCTCACGAGTGCA 524 211 334 Antisense
AAAGTACCCGGAGCCAACAGGACAG 13 1 405 Antisense
ACAGGACAGCTAAAGTGCCCACATC 670 107 421 Antisense
Sequence Source Affymetrix Proprietary Database