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[189086_s_at]

Description W04D2.1A /REP_DB=WormBase Gene ID /WP=CE06539 /TR=Q23158 /GB=CAA99941.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=alpha-actinin
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 42      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 24      6grp_total > 30      4grp_total > 60      6grp_highest_sub > 02_03      6grp_cgc > 73      6grp_high1_sub > 02_03      6grp_cgc_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2219
1 2 3 4 range
A 1993.5
(1.8)
P 		2423.8
(3)
P 		2296.7
(2.6)
P 		300.3
(-3)
P 		2124
B 1907.9
(2.1)
P 		2200.4
(3)
P 		2181
(2.9)
P 		255.8
(-3)
P 		1945
C 1764.8
(1.3)
P 		2296.5
(2.7)
P 		2388.2
(3)
P 		204.8
(-3)
P 		2183
avr 1888.7
(1.8) 		2306.9
(3) 		2288.6
(2.9) 		253.6
(-3) 		2053

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2905
1 2 3 4 5 6 range
A 3014.6
(2)
P 		2753.8
(1.1)
P 		3330.9
(3)
P 		2384.6
(-0.1)
P 		3244.6
(2.7)
P 		1513.7
(-3)
P 		1817
B 2840.9
(-1.4)
P 		3880.9
(1.5)
P 		4418.9
(3)
P 		3799.5
(1.3)
P 		2824.2
(-1.5)
P 		2287.8
(-3)
P 		2131
C 2069.4
(-2.5)
P 		4195.6
(3)
P 		2992.3
(-0.1)
P 		3967
(2.4)
P 		2067
(-2.5)
P 		1864.8
(-3)
P 		2331
avr 2641.6
(-0.4) 		3610.1
(3) 		3580.7
(2.9) 		3383.7
(2.2) 		2711.9
(-0.1) 		1888.8
(-3) 		1721
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 798
1 2 3 4 range
A 725.8
(3)
P 		526.1
(1.2)
P 		600.4
(1.9)
P 		44
(-3)
P 		682
B 608.4
(3)
P 		543.5
(2.3)
P 		505.4
(1.9)
P 		54.5
(-3)
P 		554
C 584.2
(1.1)
P 		660.3
(1.7)
P 		832.4
(3)
P 		34.5
(-3)
P 		798
avr 639.5
(2.9) 		576.6
(2.3) 		646.1
(3) 		44.3
(-3) 		602

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 923
1 2 3 4 5 6 range
A 555.7
(-1.2)
P 		979.4
(3)
P 		725.4
(0.5)
P 		604.8
(-0.7)
P 		979.1
(3)
P 		368.5
(-3)
P 		611
B 988.5
(-1.1)
P 		1291.8
(3)
P 		1073
(0)
P 		1006.4
(-0.9)
P 		1147.4
(1)
P 		853.1
(-3)
P 		439
C 767
(-1.7)
P 		1280.2
(3)
P 		761.6
(-1.7)
P 		797.8
(-1.4)
P 		668.8
(-2.6)
P 		624.2
(-3)
P 		656
avr 770.4
(-1.4) 		1183.8
(3) 		853.3
(-0.5) 		803
(-1) 		931.8
(0.3) 		615.3
(-3) 		569
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189086_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14336
Sequence Type Exemplar sequence
Representative Public ID W04D2.1A NCBI
Target Description W04D2.1A /REP_DB=WormBase Gene ID /WP=CE06539 /TR=Q23158 /GB=CAA99941.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=alpha-actinin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073726(11), NM_073727(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073726 NCBI Caenorhabditis elegans AcTiniN family member (atn-1) (atn-1) mRNA, complete cds. 11/11 None
NM_073727 NCBI Caenorhabditis elegans AcTiniN family member (atn-1) (atn-1) mRNA, complete cds. 11/11 None
SNAP00000021794 ENSEMBL cdna:SNAP chromosome:CEL140:V:12483704:12486635:1 11/11 None
GENEFINDER00000021800 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12483704:12486635:1 11/11 None
W04D2.1a.2 ENSEMBL cdna:known chromosome:CEL140:V:12480999:12487216:1 gene:W04D2.1 11/11 None
W04D2.1a.1 ENSEMBL cdna:known chromosome:CEL140:V:12481001:12487216:1 gene:W04D2.1 11/11 None
W04D2.1b ENSEMBL cdna:known chromosome:CEL140:V:12481004:12486635:1 gene:W04D2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12481003-12486635(+) 100.0 100.0
Public Domain and Genome References
Ensembl W04D2.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA99941 Hypothetical protein W04D2.1a [Caenorhabditis elegans] ref|NP_506127.1| AcTiniN family member (atn-1) [Caenorhabditis elegans] 0.0
blast AAB20685 alpha-actinin=actin-binding protein [Caenorhabditis elegans=nematode, Peptide Partial, 910 aa] 0.0
blast CAA99944 Hypothetical protein W04D2.1b [Caenorhabditis elegans] ref|NP_506128.1| AcTiniN family member (atn-1) [Caenorhabditis elegans] 0.0
blast CAE75496 Hypothetical protein CBG23504 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.8E-30
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 3.2E-38
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.8E-8
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.1E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 7.5E-9
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.6E-39
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.8E-8
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.1E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 7.5E-9
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.8E-8
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.1E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 7.5E-9
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.0E-4
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.1E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 7.5E-9
Sequence
Target Sequence 
>C. ELEGANS:189086_S_AT
aatggaatcgcttcgtgtcggatgggaaactctccttaccagtatcaacaaaacgattaa
tgaattcgaaaatcaagtattgctccgtgacagtaaaggaatcagtgaagaacaaatcgc
tgagttccgtgcgtcattcagtcattttgacaaggaacgtgctggtttggaccctgaaca
acttagatcttgcctcatttccgttggctatacgatcagaccaggacgtgaaggcgatgc
ggaacttcatcgtgttttggcacacgttgatccaaaccggatgggacgtgtaccatttga
ggcattcctcgatttcatgaccaaagaaaactcggatcaagatacggttgaacagatgat
tgattcattcagaatacttgcatctggaaagacattcataaccgctgatgaattggaacg
agaattaccacgcgatcaagcagcttactgtatggcacgaatggctccatcaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGGAATCGCTTCGTGTCGGATGG 506 161 2235 Antisense
GTCGGATGGGAAACTCTCCTTACCA 352 471 2251 Antisense
AAATCGCTGAGTTCCGTGCGTCATT 106 119 2348 Antisense
CGTGCTGGTTTGGACCCTGAACAAC 477 245 2392 Antisense
TCTTGCCTCATTTCCGTTGGCTATA 104 617 2422 Antisense
GCGGAACTTCATCGTGTTTTGGCAC 167 293 2473 Antisense
TGGCACACGTTGATCCAAACCGGAT 203 545 2492 Antisense
GACGTGTACCATTTGAGGCATTCCT 287 377 2519 Antisense
GCATTCCTCGATTTCATGACCAAAG 535 307 2536 Antisense
ATTACCACGCGATCAAGCAGCTTAC 437 11 2658 Antisense
GTATGGCACGAATGGCTCCATCAAG 479 455 2684 Antisense
Sequence Source Affymetrix Proprietary Database