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[189078_at]

Description Y6D11A.1 /REP_DB=WormBase Gene ID /WP=CE26236 /TR=Q9N4P9 /GB=AAF60863.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=ribonuclease
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 10      4grp_highest_8_8 > 33      4grp_high_8_8 > 59      6grp_highest_nonorm > 24      6grp_total > 41      4grp_total > 60      6grp_cgc > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2567
1 2 3 4 range
A 3222.6
(2.7)
P 		3237
(2.8)
P 		3326.8
(3)
P 		1073.1
(-3)
P 		2254
B 3524.9
(3)
P 		2707.1
(0.9)
P 		3339.4
(2.5)
P 		1136.8
(-3)
P 		2388
C 3288.9
(3)
P 		3126
(2.6)
P 		3188.4
(2.7)
P 		958.1
(-3)
P 		2331
avr 3345.5
(3) 		3023.4
(2.2) 		3284.9
(2.8) 		1056
(-3) 		2290

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2342
1 2 3 4 5 6 range
A 3762.2
(1.8)
P 		2859.5
(-0.5)
P 		4245.1
(3)
P 		3763.5
(1.8)
P 		2950.7
(-0.3)
P 		1903.1
(-3)
P 		2342
B 3887.1
(3)
P 		3538.2
(0.8)
P 		3526.2
(0.7)
P 		3000.9
(-2.7)
P 		3297.9
(-0.8)
P 		2955.7
(-3)
P 		931
C 3151.4
(-0.4)
P 		3679.8
(1.6)
P 		3328.7
(0.3)
P 		4068.1
(3)
P 		2539.3
(-2.6)
P 		2426.8
(-3)
P 		1641
avr 3600.2
(2.5) 		3359.2
(1.4) 		3700
(3) 		3610.8
(2.6) 		2929.3
(-0.6) 		2428.5
(-3) 		1272
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1016
1 2 3 4 range
A 1173.3
(3)
P 		702.7
(0.2)
P 		869.7
(1.2)
P 		157.1
(-3)
P 		1016
B 1124.1
(3)
P 		668.6
(-0.1)
P 		773.8
(0.6)
P 		242.2
(-3)
P 		882
C 1088.7
(2.9)
P 		898.8
(1.7)
P 		1111.3
(3)
P 		161.6
(-3)
P 		950
avr 1128.7
(3) 		756.7
(0.6) 		918.3
(1.7) 		187
(-3) 		942

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 889
1 2 3 4 5 6 range
A 693.5
(-0.5)
P 		1017
(3)
P 		924.5
(2)
P 		954.5
(2.3)
P 		890.4
(1.6)
P 		463.3
(-3)
P 		554
B 1352.5
(3)
P 		1177.7
(1.1)
P 		856.3
(-2.3)
P 		794.8
(-3)
P 		1339.9
(2.9)
P 		1102.2
(0.3)
P 		558
C 1168.1
(3)
P 		1122.8
(2.2)
P 		847.2
(-2.4)
P 		818.1
(-2.9)
P 		821.7
(-2.8)
P 		812.4
(-3)
P 		356
avr 1071.4
(2.3) 		1105.8
(3) 		876
(-1.4) 		855.8
(-1.8) 		1017.3
(1.3) 		792.6
(-3) 		313
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189078_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8162
Sequence Type Exemplar sequence
Representative Public ID Y6D11A.1 NCBI
Target Description Y6D11A.1 /REP_DB=WormBase Gene ID /WP=CE26236 /TR=Q9N4P9 /GB=AAF60863.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=ribonuclease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171077(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171077 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-286) (tag-286) mRNA, complete cds. 11/11 None
SNAP00000018128 ENSEMBL cdna:SNAP chromosome:CEL140:III:862298:865250:1 11/11 None
GENEFINDER00000018131 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:862298:865250:1 11/11 None
Y6D11A.2 ENSEMBL cdna:known chromosome:CEL140:III:862294:865350:1 gene:Y6D11A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:860189-865250(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-286
Ensembl Y6D11A.2
Entrez Gene 175247 Entrez gene
SwissProt Q8WTM6 EMBL-EBI
WormBase CE30060 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264498_AT actin-related protein 2/3 complex 34kDa subunit family / arp2/3 complex 34kDa subunit family at
CANINE_2:CFAAFFX.22531.1.S1_AT similar to actin related protein 2/3 complex subunit 2 cfa
DROSOPHILA_2:1623402_AT dm
DROSGENOME1:154503_AT dm
CHICKEN:GGAAFFX.23567.1.S1_AT similar to actin related protein 2/3 complex subunit 2; ARP2/3 protein complex subunit 34; actin related protein 2/3 complex, subunit 2 (34 kD) gga
HG-U95AV2:1718_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HC-G110:1718_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U95B:44310_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133_PLUS_2:207988_S_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133A:207988_S_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133A_2:207988_S_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133_PLUS_2:208679_S_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133A_2:208679_S_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133A:208679_S_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HU35KSUBD:RC_N29963_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HUGENEFL:U50523_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
U133_X3P:G12751114_3P_A_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
U133_X3P:HS.252280.1.A1_3P_S_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133A_2:213513_X_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133_PLUS_2:213513_X_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
HG-U133A:213513_X_AT actin related protein 2/3 complex, subunit 2, 34kDa hs
MG-U74AV2:93288_AT actin related protein 2/3 complex, subunit 2 mm
MOE430A:1437148_AT actin related protein 2/3 complex, subunit 2 mm
MOUSE430A_2:1437148_AT actin related protein 2/3 complex, subunit 2 mm
MOUSE430_2:1437148_AT actin related protein 2/3 complex, subunit 2 mm
MG-U74CV2:134216_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MG-U74CV2:137570_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MOE430B:1442295_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MOUSE430_2:1442295_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU19KSUBA:TC15156_R_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU19KSUBA:TC19253_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU19KSUBA:TC19253_G_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU19KSUBA:TC19254_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU19KSUBA:TC19254_G_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU19KSUBA:TC24917_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU19KSUBC:TC41917_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU19KSUBC:TC41917_G_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
MU11KSUBB:W30625_AT Actin related protein 2/3 complex, subunit 2, mRNA (cDNA clone IMAGE:30714846) mm
RG-U34B:RC_AI070323_AT actin related protein 2/3 complex, subunit 2 (predicted) rn
RG-U34C:RC_AI175467_AT actin related protein 2/3 complex, subunit 2 (predicted) rn
RAE230A:1375137_AT actin related protein 2/3 complex, subunit 2 (predicted) rn
RAT230_2:1375137_AT actin related protein 2/3 complex, subunit 2 (predicted) rn
RAE230A:1371511_AT actin related protein 2/3 complex, subunit 2 (predicted) rn
RAT230_2:1371511_AT actin related protein 2/3 complex, subunit 2 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
30833 regulation of actin filament polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5856 cytoskeleton inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAL32261 Hypothetical protein Y6D11A.2 [Caenorhabditis elegans] ref|NP_741088.1| Temporarily Assigned Gene name family member (tag-286) [Caenorhabditis elegans] sp|Q8WTM6|ARPC2_CAEEL Probable actin-related protein 2/3 complex subunit 2 (ARP2/3 complex 34 kDa subunit) (p34-ARC) 1.0E-171
blast CAE56478 Hypothetical protein CBG24192 [Caenorhabditis briggsae] 1.0E-158
blast AAI05137 MGC126898 protein [Bos taurus] ref|NP_001029885.1| hypothetical protein LOC540838 [Bos taurus] pdb|1U2V|D Chain D, Crystal Structure Of Arp23 COMPLEX WITH BOUND ADP AND Calcium pdb|1TYQ|D Chain D, Crystal Structure Of Arp23 COMPLEX WITH BOUND ATP AND Calcium pdb|1K8K|D Chain D, Crystal Structure Of Arp23 COMPLEX 1.0E-119
Protein Domains
Database ID Description E-Value
Pfam IPR007188 EMBL-EBI Arp2/3 complex, 34kDa subunit p34-Arc 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189078_AT
gaatacaatttggccgatcttccggcagatacaacggatttggtgcaggcagcttctgct
ttgaagagaaattgttttgcttcagtctttgaaaagtatttcgaattccaagaggccggc
caggaggggcataagagagctgttattaattatagagatgatgagacaatgtacattgaa
gcgaaagcggatcgtgtgaccgtaatcttcagtacagttttcaaggacgctgacgatgtg
ataatcggaaaagtgttccttcaagaattccgtgaaggccgaaaagccagtcaaactgct
ccagcagtactctactcactcggtgaaccacctttggagcttaaagatcttccagaagcc
cgtgttggagacaatgttggatacattacatttgtgttgttcccacgtcacacaaataag
aagacaaaagacaatacgattgatttgattcattcgttccgtgactatcttcattatcat
atcaaatgttccaaagtctaccttcacaccagaatgcgtgccaagaccactgatttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATACAATTTGGCCGATCTTCCGG 321 327 1057 Antisense
GGTGCAGGCAGCTTCTGCTTTGAAG 622 499 1098 Antisense
ATTTCGAATTCCAAGAGGCCGGCCA 12 15 1154 Antisense
GCGGATCGTGTGACCGTAATCTTCA 497 293 1243 Antisense
GTACTCTACTCACTCGGTGAACCAC 502 459 1363 Antisense
GATCTTCCAGAAGCCCGTGTTGGAG 531 415 1402 Antisense
TACATTTGTGTTGTTCCCACGTCAC 513 643 1443 Antisense
GATTCATTCGTTCCGTGACTATCTT 212 431 1503 Antisense
AATGTTCCAAAGTCTACCTTCACAC 441 163 1541 Antisense
TACCTTCACACCAGAATGCGTGCCA 138 649 1555 Antisense
ATGCGTGCCAAGACCACTGATTTCT 68 41 1570 Antisense
Sequence Source Affymetrix Proprietary Database