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[189074_at] Synaptotagmin

Description F42G9.7 /REP_DB=WormBase Gene ID /WP=CE07234 /TR=Q20354 /GB=AAA91359.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Synaptotagmin
Cluster 6grp_total > 90      4grp_total > 14      6grp_cgc > 26      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 37.3
(3)
A 		26.7
(-0.3)
A 		17.9
(-3)
A 		22.2
(-1.7)
A 		19
B 40.6
(3)
A 		7.9
(-3)
A 		18.1
(-1.1)
A 		15
(-1.7)
A 		33
C 7.2
(-2.7)
A 		4.9
(-3)
A 		5.2
(-3)
A 		59.7
(3)
A 		55
avr 28.4
(1.8) 		13.2
(-3) 		13.7
(-2.8) 		32.3
(3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 12.4
(-0.6)
A 		9.5
(-3)
A 		11.1
(-1.7)
A 		16.9
(3)
A 		11.7
(-1.2)
A 		9.8
(-2.8)
A 		7
B 44.2
(2.5)
A 		11.1
(-2.3)
A 		6.6
(-3)
A 		26
(-0.1)
A 		9.5
(-2.6)
A 		47.3
(3)
A 		41
C 6.5
(-2.6)
A 		21.1
(-0.6)
A 		11.7
(-1.9)
A 		47.8
(3)
A 		3.8
(-3)
A 		23.5
(-0.3)
A 		44
avr 21
(0.5) 		13.9
(-1.5) 		9.8
(-2.6) 		30.2
(3) 		8.3
(-3) 		26.9
(2.1) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 13.6
(3)
A 		5.8
(-1.5)
A 		4.7
(-2.1)
A 		3.2
(-3)
A 		10
B 13
(3)
A 		2
(-3)
A 		4.2
(-1.8)
A 		3.2
(-2.3)
A 		11
C 2.4
(-2.3)
A 		1.4
(-3)
A 		1.8
(-2.7)
A 		10.1
(3)
A 		9
avr 9.7
(3) 		3.1
(-3) 		3.6
(-2.5) 		5.5
(-0.8) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 2.3
(-3)
A 		3.4
(0.3)
A 		2.4
(-2.7)
A 		4.3
(3)
A 		3.5
(0.6)
A 		2.4
(-2.7)
A 		2
B 15.4
(2.2)
A 		3.7
(-2.2)
A 		1.6
(-3)
A 		6.9
(-1)
A 		3.8
(-2.2)
A 		17.6
(3)
A 		16
C 2.4
(-2.1)
A 		6.4
(0.7)
A 		3
(-1.7)
A 		9.6
(3)
A 		1.2
(-3)
A 		7.9
(1.8)
A 		8
avr 6.7
(0.8) 		4.5
(-1.1) 		2.3
(-3) 		6.9
(0.9) 		2.8
(-2.6) 		9.3
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189074_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8130
Sequence Type Exemplar sequence
Representative Public ID F42G9.7 NCBI
Target Description F42G9.7 /REP_DB=WormBase Gene ID /WP=CE07234 /TR=Q20354 /GB=AAA91359.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Synaptotagmin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064860(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064860 NCBI Caenorhabditis elegans F42G9.7 (Synaptotagmin) mRNA, complete cds. 11/11 None
F42G9.7 ENSEMBL cdna:known chromosome:CEL140:III:786097:793681:-1 gene:F42G9.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000002851 2/11 Cross Hyb Matching Probes None
SNAP00000002841 2/11 Cross Hyb Matching Probes None
SNAP00000038500 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:786692-789647(-) 100.0 100.0
Public Domain and Genome References
Gene Title Synaptotagmin
Gene Symbol snt-2
Ensembl F42G9.7
Entrez Gene 185680 Entrez gene
WormBase CE37915 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8021 synaptic vesicle inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_497261 F42G9.7 [Caenorhabditis elegans] 0.0
blast AAZ91344 Synaptotagmin protein 2 [Caenorhabditis elegans] 1.0E-141
blast T16355 hypothetical protein F42G9.7 - Caenorhabditis elegans 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 1.4E-41
Pfam IPR000008 EMBL-EBI C2 domain 3.1E-37
Sequence
Target Sequence 
>C. ELEGANS:189074_AT
taagatgggtcaactgtccgtgccactggagtcaattgattttggaattactactgatat
cgaacgacctcttcagaagcccgagaaagacgatgagaaagaatgccgactcggtgatat
ttgcttctccactcggtaccgacctgcaaccggtacagtaacccttacgatcatggaggc
caggaatttgaaaaagatggatgttggtggttcttcagatccatacgtaaaaatctactt
acaccacggacgaaagctgttaagtaagaaaaagacgtcgagaaagtacaaaaccctgaa
tccctactacaatgagagtttccaattcaaaattgagccacatatgattgagaaagttca
tctgattgtgtccgtctgggattatgacaagatgagcaagaacgactttatcggagaggt
gacgttgggctccaagcatttgaatttaccgcagatcacccacgcgtgcagcgaacagtg
ggctgaaatgatgacgtcacggaggcctgtagttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAGATGGGTCAACTGTCCGTGCCA 374 637 216 Antisense
TATCGAACGACCTCTTCAGAAGCCC 584 647 273 Antisense
GACTCGGTGATATTTGCTTCTCCAC 276 375 323 Antisense
GACCTGCAACCGGTACAGTAACCCT 152 383 356 Antisense
GTTGGTGGTTCTTCAGATCCATACG 431 437 418 Antisense
ACCCTGAATCCCTACTACAATGAGA 264 91 508 Antisense
TTCATCTGATTGTGTCCGTCTGGGA 580 687 572 Antisense
AGGTGACGTTGGGCTCCAAGCATTT 50 55 632 Antisense
TTTGAATTTACCGCAGATCACCCAC 572 665 654 Antisense
CACCCACGCGTGCAGCGAACAGTGG 328 201 672 Antisense
ATGACGTCACGGAGGCCTGTAGTTC 359 45 706 Antisense
Sequence Source Affymetrix Proprietary Database