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[189072_at] galactokinase

Description M01D7.4 /REP_DB=WormBase Gene ID /WP=CE26379 /TR=O01969 /GB=AAB58070.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=galactokinase
Cluster 6grp_high1_10_10 > 81      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_total > 83      4grp_total > 41      6grp_cgc > 87      6grp_high1_sub > 04      6grp_cgc_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 157.5
(-3)
P 		210.9
(0.5)
P 		248.4
(3)
P 		228.4
(1.7)
P 		91
B 132.5
(-3)
P 		206.1
(0.3)
P 		267
(3)
P 		151.6
(-2.1)
P 		135
C 120.5
(-3)
P 		259.4
(3)
P 		230.7
(1.8)
P 		169.4
(-0.9)
P 		139
avr 136.8
(-3) 		225.5
(1.8) 		248.7
(3) 		183.1
(-0.5) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 310
1 2 3 4 5 6 range
A 152.5
(-2.1)
P 		147.9
(-2.2)
P 		235.7
(-0.1)
P 		289
(1.2)
P 		363.6
(3)
P 		114
(-3)
P 		250
B 97.1
(-3)
A 		169.4
(-1.6)
P 		297.2
(0.9)
P 		347.4
(1.8)
P 		406.9
(3)
P 		210.1
(-0.8)
P 		310
C 169.3
(-2.5)
P 		201.9
(-1.3)
P 		155.8
(-3)
P 		319.5
(3)
P 		245.7
(0.3)
P 		239
(0)
P 		164
avr 139.6
(-3) 		173.1
(-2) 		229.6
(-0.3) 		318.6
(2.4) 		338.7
(3) 		187.7
(-1.6) 		199
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 57.3
(1.6)
P 		45.8
(-0.6)
P 		64.9
(3)
P 		33.4
(-3)
P 		32
B 42.3
(-1)
P 		50.9
(0.8)
P 		61.9
(3)
P 		32.3
(-3)
P 		30
C 39.9
(-1.7)
P 		74.6
(2.3)
P 		80.4
(3)
P 		28.6
(-3)
P 		52
avr 46.5
(-0.6) 		57.1
(1.1) 		69.1
(3) 		31.4
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 28.1
(-3)
P 		52.6
(-1.2)
P 		51.3
(-1.3)
P 		73.3
(0.3)
P 		109.7
(3)
P 		27.8
(-3)
P 		82
B 33.8
(-3)
A 		56.4
(-2)
P 		72.2
(-1.2)
P 		92
(-0.3)
P 		165.3
(3)
P 		78.3
(-1)
P 		132
C 62.7
(0.4)
P 		61.6
(0.3)
P 		39.7
(-3)
P 		64.3
(0.7)
P 		79.5
(2.9)
P 		80
(3)
P 		40
avr 41.5
(-3) 		56.9
(-1.8) 		54.4
(-2) 		76.5
(-0.3) 		118.2
(3) 		62
(-1.4) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189072_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.164
Sequence Type Exemplar sequence
Representative Public ID M01D7.4 NCBI
Target Description M01D7.4 /REP_DB=WormBase Gene ID /WP=CE26379 /TR=O01969 /GB=AAB58070.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=galactokinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058508(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058508 NCBI Caenorhabditis elegans M01D7.4 (galactokinase) mRNA, complete cds. 11/11 None
SNAP00000009590 ENSEMBL cdna:SNAP chromosome:CEL140:I:1845222:1850403:1 11/11 None
GENEFINDER00000009604 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1845222:1850403:1 11/11 None
M01D7.4 ENSEMBL cdna:known chromosome:CEL140:I:1845222:1850452:1 gene:M01D7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1845221-1850403(+) 97.38 100.0
Public Domain and Genome References
Gene Title galactokinase
Ensembl M01D7.4
Entrez Gene 171755 Entrez gene
SwissProt O01969 EMBL-EBI
WormBase CE36705 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258517_AT galactokinase (GAL1) at
CANINE:1600523_AT similar to galactokinase 2 isoform 2 cfa
CANINE:1590000_AT similar to galactokinase 2 isoform 2 cfa
CANINE_2:CFA.6718.1.A1_AT similar to galactokinase 2 isoform 2 cfa
CANINE_2:CFAAFFX.22935.1.S1_S_AT similar to galactokinase 2 isoform 2 cfa
DROSOPHILA_2:1641681_S_AT dm
DROSGENOME1:152351_AT dm
CHICKEN:GGAAFFX.11450.1.S1_AT galactokinase 2 gga
CHICKEN:GGAAFFX.8771.1.S1_S_AT galactokinase 2 gga
CHICKEN:GGAAFFX.8772.1.S1_S_AT galactokinase 2 gga
CHICKEN:GGAAFFX.11450.1.A1_AT galactokinase 2 gga
HG-U133_PLUS_2:216411_S_AT galactokinase 2 hs
HG-U133A_2:216411_S_AT galactokinase 2 hs
HG-U133A:216411_S_AT galactokinase 2 hs
HU35KSUBA:M84443_S_AT galactokinase 2 hs
HG-U133A:205219_S_AT galactokinase 2 hs
HG-U133_PLUS_2:205219_S_AT galactokinase 2 hs
HG-FOCUS:205219_S_AT galactokinase 2 hs
HG-U133A_2:205219_S_AT galactokinase 2 hs
U133_X3P:G4503896_3P_S_AT galactokinase 2 hs
U133_X3P:HS.287584.1.A1_3P_A_AT galactokinase 2 hs
HG-U95AV2:37825_AT galactokinase 2 hs
HG-U95D:87903_AT galactokinase 2 hs
U133_X3P:HS2.380391.2.S1_3P_S_AT Galactokinase 2 hs
HG-U133_PLUS_2:1557429_S_AT Galactokinase 2 hs
HG-U95D:69597_AT Galactokinase 2 hs
MU11KSUBB:MSA.4244.0_S_AT galactokinase 2 mm
MG-U74AV2:160121_AT galactokinase 2 mm
MOE430A:1455798_AT galactokinase 2 mm
MOUSE430A_2:1455798_AT galactokinase 2 mm
MOUSE430_2:1455798_AT galactokinase 2 mm
MOUSE430_2:1438642_AT Galactokinase 2, mRNA (cDNA clone MGC:100208 IMAGE:5690056) mm
MOE430A:1438642_AT Galactokinase 2, mRNA (cDNA clone MGC:100208 IMAGE:5690056) mm
MOUSE430A_2:1438642_AT Galactokinase 2, mRNA (cDNA clone MGC:100208 IMAGE:5690056) mm
MU11KSUBB:MSA.39248.0_S_AT Galactokinase 2, mRNA (cDNA clone MGC:100208 IMAGE:5690056) mm
MU19KSUBB:TC26264_AT Galactokinase 2, mRNA (cDNA clone MGC:100208 IMAGE:5690056) mm
MU19KSUBB:TC32231_AT Galactokinase 2, mRNA (cDNA clone MGC:100208 IMAGE:5690056) mm
MU19KSUBC:TC35541_AT Galactokinase 2, mRNA (cDNA clone MGC:100208 IMAGE:5690056) mm
MU19KSUBC:TC35541_G_AT Galactokinase 2, mRNA (cDNA clone MGC:100208 IMAGE:5690056) mm
YEAST_2:1778677_AT Transcriptional regulator involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p and Gal4p to relieve inhibition by Gal80p; binds galactose and ATP but does not have galactokinase activity Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6012 galactose metabolism inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
16310 phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4335 galactokinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
16301 kinase activity inferred from electronic annotation QuickGO AmiGO
16773 phosphotransferase activity, alcohol group as acceptor inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK84629 Hypothetical protein M01D7.4 [Caenorhabditis elegans] ref|NP_490909.2| M01D7.4 [Caenorhabditis elegans] 0.0
blast T15285 hypothetical protein M01D7.4 - Caenorhabditis elegans 0.0
blast CAE74470 Hypothetical protein CBG22217 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006204 EMBL-EBI GHMP kinase 3.6E-37
Pfam IPR006204 EMBL-EBI GHMP kinase 5.4E-35
Sequence
Target Sequence 
>C. ELEGANS:189072_AT
gaataagggtgccacgtcacattataacgaaagggttattgaaggacggattgttgcaca
aattttcaaacaaaaattcaatatcacaagtggctcatttcgcctcaaagaaattcaaac
cttaagcggaaaatcgttcaaggaaattttgaaaattgtcgaggagctaccggatgaagt
caataaagagcaagttattgatctaattggtgccgataaattggaagaatgtcttacaga
gaatacaagatcattccaaaaattcaaactccgcccccgcgctcgtcacgtgttcagtga
ggcctatcgtgtggaacaattcgaatcggcgtgcgcccagaaaaacattcaagaaatggg
acgtctaatgaacgagagtcatcgcagttgtgccattgactacgagtgcagttgtcggga
gcttgacgagatttgtcggctctatttggaccatggagctctcggagcacggcttaccgg
agccggttggggaggatgtgccgtcgtgttgatggccgctgatgacgtggatcgggtggt
tgccgagttgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATAAGGGTGCCACGTCACATTAT 624 283 744 Antisense
ATCACAAGTGGCTCATTTCGCCTCA 340 27 826 Antisense
GTGTTCAGTGAGGCCTATCGTGTGG 545 485 1033 Antisense
AATTCGAATCGGCGTGCGCCCAGAA 647 177 1061 Antisense
GAGAGTCATCGCAGTTGTGCCATTG 438 395 1117 Antisense
GAGTGCAGTTGTCGGGAGCTTGACG 328 401 1147 Antisense
GACGAGATTTGTCGGCTCTATTTGG 378 377 1168 Antisense
CTATTTGGACCATGGAGCTCTCGGA 96 223 1185 Antisense
CGGAGCCGGTTGGGGAGGATGTGCC 664 239 1221 Antisense
AGGATGTGCCGTCGTGTTGATGGCC 384 57 1236 Antisense
TGGATCGGGTGGTTGCCGAGTTGCC 285 549 1271 Antisense
Sequence Source Affymetrix Proprietary Database